公开(公告)号：US20090098582A1

公开(公告)日：2009-04-16

申请号：US12249417

申请日：2008-10-10

申请人： Tomoko OYAMA ,  Michitaka Notoya ,  Tomokazu Yoshida ,  Hideki Ishihara

发明人： Tomoko OYAMA ,  Michitaka Notoya ,  Tomokazu Yoshida ,  Hideki Ishihara

IPC分类号： G01N33/53 ,  G06F19/00

CPC分类号： G01N33/57484 ,  G01N2333/91205 ,  G01N2800/54 ,  G16H50/20 ,  Y02A90/26

摘要： The invention provides a method for determining a risk of cancer relapse comprising a step of determining the risk of cancer relapse on the basis of a comprehensive activity value of a transmembrane tyrosine kinase of a tumor cell, as well as a computer program product for determining a risk of a cancer relapse.

摘要翻译： 本发明提供了一种确定癌症复发风险的方法，该方法包括基于肿瘤细胞的跨膜酪氨酸激酶的综合活性值确定癌症复发的风险的步骤，以及用于确定 癌症复发的风险。

公开(公告)号：US20080003630A1

公开(公告)日：2008-01-03

申请号：US11819236

申请日：2007-06-26

申请人： Michitaka Notoya ,  Tomoko Ohyama ,  Tomokazu Yoshida ,  Hideki Ishihara

发明人： Michitaka Notoya ,  Tomoko Ohyama ,  Tomokazu Yoshida ,  Hideki Ishihara

IPC分类号： C12Q1/48 ,  C07K14/00

CPC分类号： C07K14/71 ,  C07K1/13 ,  C07K2319/00 ,  C12Q1/485

摘要： A tyrosine kinase substrate is described. The tyrosine kinase substrate comprises a fusion protein which comprises a protein for labeling and a specific peptide fused with the protein for labeling. The specific peptide has an amino acid sequence including a glutamic acid residue and a tyrosine residue. The tyrosine kinase substrate is capable of being phosphorylated by a plurality of kinds of tyrosine kinases.

摘要翻译： 描述了酪氨酸激酶底物。 酪氨酸激酶底物包含融合蛋白，其包含用于标记的蛋白质和与用于标记的蛋白质融合的特异性肽。 特定肽具有包含谷氨酸残基和酪氨酸残基的氨基酸序列。 酪氨酸激酶底物能够被多种酪氨酸激酶磷酸化。

公开(公告)号：US20080090254A1

公开(公告)日：2008-04-17

申请号：US11905027

申请日：2007-09-27

申请人： Michitaka Notoya ,  Jun Sato ,  Tomoko Ohyama ,  Tomokazu Yoshida ,  Hideki Ishihara

发明人： Michitaka Notoya ,  Jun Sato ,  Tomoko Ohyama ,  Tomokazu Yoshida ,  Hideki Ishihara

IPC分类号： G01N33/53

CPC分类号： C12Q1/485 ,  G01N2333/9121 ,  G01N2500/00

摘要： A method for assessing the proliferation inhibiting effect of a receptor tyrosine kinase inhibitor is described. In the method, a cytoplasm is separated from a tumor cell to prepare a sample containing various receptor tyrosine kinases. The prepared sample is treated with the inhibitor. The receptor tyrosine kinases in the treated sample, and a substrate for at least two kinds of receptor tyrosine kinases are contacted. The phosphorylated substrate is detected, and the activity value of the receptor tyrosine kinases is measured based on the detection result. The proliferation inhibiting effect is assessed based on the resulting activity value.

摘要翻译： 描述了用于评估受体酪氨酸激酶抑制剂的增殖抑制作用的方法。 在该方法中，从肿瘤细胞分离细胞质以制备含有各种受体酪氨酸激酶的样品。 制备的样品用抑制剂处理。 处理的样品中的受体酪氨酸激酶和至少两种受体酪氨酸激酶的底物接触。 检测磷酸化底物，并根据检测结果测定受体酪氨酸激酶的活性值。 基于得到的活性值评估增殖抑制作用。

公开(公告)号：US20070238141A1

公开(公告)日：2007-10-11

申请号：US11729901

申请日：2007-03-30

申请人： Michitaka Notoya ,  Tomoko Ohyama ,  Shigeyuki Tamura ,  Tomokazu Yoshida ,  Hideki Ishihara

发明人： Michitaka Notoya ,  Tomoko Ohyama ,  Shigeyuki Tamura ,  Tomokazu Yoshida ,  Hideki Ishihara

IPC分类号： C12Q1/48 ,  G01N33/53

CPC分类号： C12Q1/485 ,  G01N33/573

摘要： A method for measuring the activity of a receptor kinase present in a cell membrane of a cell is described. The method comprises steps of preparing a sample containing the receptor kinase by separating a cytoplasm from the cell, contacting the receptor kinase in the sample with a substrate to phosphorylate the substrate with the activity of the receptor kinase, binding a labeling substance to the phosphorylated substrate, and measuring the activity of the receptor kinase based on a result of detection of the labeling substance bound to the phosphorylated substrate.

摘要翻译： 描述了测定存在于细胞的细胞膜中的受体激酶的活性的方法。 该方法包括以下步骤：通过从细胞分离细胞质来制备含有受体激酶的样品，使样品中的受体激酶与底物接触，以使受体激酶活性磷酸化底物，将标记物质结合到磷酸化底物 ，并且基于与磷酸化底物结合的标记物质的检测结果测量受体激酶的活性。

公开(公告)号：US20110027809A1

公开(公告)日：2011-02-03

申请号：US12934978

申请日：2009-03-23

申请人： Michitaka Notoya ,  Jun Sato ,  Hideki Ishihara

发明人： Michitaka Notoya ,  Jun Sato ,  Hideki Ishihara

IPC分类号： G01N33/574

CPC分类号： C12Q1/485 ,  G01N33/57492 ,  G01N2800/56

摘要： Disclosed is a method for evaluating the degree of malignancy of a tumor cell, which comprises: preparing a cell membrane fraction containing a receptor tyrosine kinase (RTK) from a sample containing the tumor cell; measuring the enzymatic activity of RTK in the cell membrane fraction in both the presence and absence of an RTK activity inhibitor; and comparing the results obtained in the presence of the RTK activity inhibitor with those obtained in the absence of the RTK activity inhibitor.

摘要翻译： 本发明公开了一种评价肿瘤细胞恶性程度的方法，包括：从含有肿瘤细胞的样品制备含有受体酪氨酸激酶（RTK）的细胞膜级分; 在存在和不存在RTK活性抑制剂的情况下测量细胞膜级分中RTK的酶活性; 并将RTK活性抑制剂存在下得到的结果与不存在RTK活性抑制剂时得到的结果进行比较。