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    Method for assessing proliferation inhibiting effect of inhibitor, and method for determining sensitivity of tumor cell to inhibitor
    1.
    发明申请
    Method for assessing proliferation inhibiting effect of inhibitor, and method for determining sensitivity of tumor cell to inhibitor 审中-公开
    评估抑制剂增殖抑制作用的方法，以及确定肿瘤细胞对抑制剂敏感性的方法

    公开(公告)号：US20080090254A1

    公开(公告)日：2008-04-17

    申请号：US11905027

    申请日：2007-09-27

    申请人： Michitaka Notoya ,  Jun Sato ,  Tomoko Ohyama ,  Tomokazu Yoshida ,  Hideki Ishihara

    发明人： Michitaka Notoya ,  Jun Sato ,  Tomoko Ohyama ,  Tomokazu Yoshida ,  Hideki Ishihara

    IPC分类号： G01N33/53

    CPC分类号： C12Q1/485 ,  G01N2333/9121 ,  G01N2500/00

    摘要： A method for assessing the proliferation inhibiting effect of a receptor tyrosine kinase inhibitor is described. In the method, a cytoplasm is separated from a tumor cell to prepare a sample containing various receptor tyrosine kinases. The prepared sample is treated with the inhibitor. The receptor tyrosine kinases in the treated sample, and a substrate for at least two kinds of receptor tyrosine kinases are contacted. The phosphorylated substrate is detected, and the activity value of the receptor tyrosine kinases is measured based on the detection result. The proliferation inhibiting effect is assessed based on the resulting activity value.

    摘要翻译： 描述了用于评估受体酪氨酸激酶抑制剂的增殖抑制作用的方法。 在该方法中，从肿瘤细胞分离细胞质以制备含有各种受体酪氨酸激酶的样品。 制备的样品用抑制剂处理。 处理的样品中的受体酪氨酸激酶和至少两种受体酪氨酸激酶的底物接触。 检测磷酸化底物，并根据检测结果测定受体酪氨酸激酶的活性值。 基于得到的活性值评估增殖抑制作用。

    Tyrosine kinse substrate
    2.
    发明申请
    Tyrosine kinse substrate 审中-公开
    酪氨酸激酶底物

    公开(公告)号：US20080003630A1

    公开(公告)日：2008-01-03

    申请号：US11819236

    申请日：2007-06-26

    申请人： Michitaka Notoya ,  Tomoko Ohyama ,  Tomokazu Yoshida ,  Hideki Ishihara

    发明人： Michitaka Notoya ,  Tomoko Ohyama ,  Tomokazu Yoshida ,  Hideki Ishihara

    IPC分类号： C12Q1/48 ,  C07K14/00

    CPC分类号： C07K14/71 ,  C07K1/13 ,  C07K2319/00 ,  C12Q1/485

    摘要： A tyrosine kinase substrate is described. The tyrosine kinase substrate comprises a fusion protein which comprises a protein for labeling and a specific peptide fused with the protein for labeling. The specific peptide has an amino acid sequence including a glutamic acid residue and a tyrosine residue. The tyrosine kinase substrate is capable of being phosphorylated by a plurality of kinds of tyrosine kinases.

    摘要翻译： 描述了酪氨酸激酶底物。 酪氨酸激酶底物包含融合蛋白，其包含用于标记的蛋白质和与用于标记的蛋白质融合的特异性肽。 特定肽具有包含谷氨酸残基和酪氨酸残基的氨基酸序列。 酪氨酸激酶底物能够被多种酪氨酸激酶磷酸化。

    Method for measuring kinase activity
    3.
    发明申请
    Method for measuring kinase activity 审中-公开
    测定激酶活性的方法

    公开(公告)号：US20070238141A1

    公开(公告)日：2007-10-11

    申请号：US11729901

    申请日：2007-03-30

    申请人： Michitaka Notoya ,  Tomoko Ohyama ,  Shigeyuki Tamura ,  Tomokazu Yoshida ,  Hideki Ishihara

    发明人： Michitaka Notoya ,  Tomoko Ohyama ,  Shigeyuki Tamura ,  Tomokazu Yoshida ,  Hideki Ishihara

    IPC分类号： C12Q1/48 ,  G01N33/53

    CPC分类号： C12Q1/485 ,  G01N33/573

    摘要： A method for measuring the activity of a receptor kinase present in a cell membrane of a cell is described. The method comprises steps of preparing a sample containing the receptor kinase by separating a cytoplasm from the cell, contacting the receptor kinase in the sample with a substrate to phosphorylate the substrate with the activity of the receptor kinase, binding a labeling substance to the phosphorylated substrate, and measuring the activity of the receptor kinase based on a result of detection of the labeling substance bound to the phosphorylated substrate.

    摘要翻译： 描述了测定存在于细胞的细胞膜中的受体激酶的活性的方法。 该方法包括以下步骤：通过从细胞分离细胞质来制备含有受体激酶的样品，使样品中的受体激酶与底物接触，以使受体激酶活性磷酸化底物，将标记物质结合到磷酸化底物 ，并且基于与磷酸化底物结合的标记物质的检测结果测量受体激酶的活性。

    EFFECT PREDICTING APPARATUS, COMPUTER PROGRAM PRODUCT, AND METHOD FOR PREDICTING AN EFFECT
    6.
    发明申请
    EFFECT PREDICTING APPARATUS, COMPUTER PROGRAM PRODUCT, AND METHOD FOR PREDICTING AN EFFECT 审中-公开
    效果预测装置，计算机程序产品和预测效果的方法

    公开(公告)号：US20100250483A1

    公开(公告)日：2010-09-30

    申请号：US12569130

    申请日：2009-09-29

    申请人： Tomoko Ohyama ,  Masaki Shibayama ,  Hideki Ishihara ,  Tomokazu Yoshida ,  Keigo Gohda

    发明人： Tomoko Ohyama ,  Masaki Shibayama ,  Hideki Ishihara ,  Tomokazu Yoshida ,  Keigo Gohda

    IPC分类号： G06N5/02

    CPC分类号： G16C20/30 ,  G16B25/00

    摘要： An effect predicting apparatus for predicting an effect of anthracycline anticancer drugs, comprising: a display, a processor, and a memory, under control of said processor, including software instructions adapted to enable the processor to perform operations, comprising: acquiring a CDK parameter based on a first CDK parameter and a second CDK parameter; acquiring an expression level of glutathione; comparing the CDK parameter with a CDK threshold value, and the expression level of glutathione with a glutathione threshold value; predicting an effect of anthracycline anticancer drugs based on the result of the comparison; and displaying the result of the prediction. A computer program product and a method for predicting an effect are also disclosed.

    摘要翻译： 一种用于预测蒽环类抗癌药物的效果的效果预测装置，包括：在所述处理器的控制下的显示器，处理器和存储器，包括适于使处理器执行操作的软件指令，包括：基于CDK参数获取 第一个CDK参数和第二个CDK参数; 获得谷胱甘肽的表达水平; 将CDK参数与CDK阈值进行比较，以及谷胱甘肽阈值的谷胱甘肽的表达水平; 基于比较结果预测蒽环类抗癌药物的作用; 并显示预测结果。 还公开了一种用于预测效果的计算机程序产品和方法。

    METHOD FOR DETERMINING ACTIVITY OF CELL CYCLE REGULATORY FACTOR AND METHOD FOR DIAGNOSING CANCER USING THE SAME
    7.
    发明申请
    METHOD FOR DETERMINING ACTIVITY OF CELL CYCLE REGULATORY FACTOR AND METHOD FOR DIAGNOSING CANCER USING THE SAME 审中-公开
    用于确定细胞周期调节因子的活性的方法和使用其诊断癌症的方法

    公开(公告)号：US20070264624A1

    公开(公告)日：2007-11-15

    申请号：US11747077

    申请日：2007-05-10

    申请人： Hideki Ishihara ,  Tomokazu Yoshida ,  Masatoshi Yamasaki ,  Sachiyo Tada

    发明人： Hideki Ishihara ,  Tomokazu Yoshida ,  Masatoshi Yamasaki ,  Sachiyo Tada

    IPC分类号： C12Q1/00

    CPC分类号： C07K16/40 ,  C12N9/1205 ,  C12Q1/48 ,  C12Q1/485 ,  G01N33/57407 ,  G01N33/57484 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/9121

    摘要： A method for determining the activity of a cell cycle regulatory factor comprising the steps of preparing a sample for measuring a cyclin-dependent kinase/cyclin complex from living cells; reacting adenosine 5′-O-(3-thiotriphosphate) (ATP-γ S) with a substrate for the cyclin-dependent kinase in presence of the sample in order to introduce a monothiophosphate group into a serine or threonine residue of the substrate; labeling the substrate by coupling a labeling fluorophore or a labeling enzyme with a sulfur atom of the introduced monothiophosphate group; measuring the amount of fluorescence from the labeling fluorophore labeling the substrate, or reacting the labeling enzyme labeling the substrate with a substance which generates an optically detectable product by reaction with the labeling enzyme and optically measuring the amount of the generated product; and calculating the activity of the cyclin-dependent kinase from the measured amount of fluorescence or the measured amount of the generated product with reference to a pre-produced reference curve.

    摘要翻译： 一种确定细胞周期调控因子活性的方法，包括以下步骤：制备用于从活细胞测量细胞周期蛋白依赖性激酶/细胞周期蛋白复合物的样品; 在样品存在下使腺苷5'-O-（3-硫代三磷酸）（ATP-γS）与用于细胞周期蛋白依赖性激酶的底物反应，以将一硫代磷酸酯基团引入底物的丝氨酸或苏氨酸残基中; 通过将标记的荧光团或标记酶与引入的一硫代磷酸酯基团的硫原子偶联来标记底物; 测量来自标记荧光团的荧光标记基质的荧光量，或使标记基质的标记酶与通过与标记酶反应产生可光学检测的产物并光学测量产生的产物的量的物质反应; 并且根据测量的荧光量或参考预先产生的参考曲线测量产生的产物的量计算细胞周期蛋白依赖性激酶的活性。

    Method for determining activity of cell cycle regulatory factor and method for diagnosing cancer using the same
    8.
    发明授权
    Method for determining activity of cell cycle regulatory factor and method for diagnosing cancer using the same 失效
    用于确定细胞周期调控因子活性的方法和使用其诊断癌症的方法

    公开(公告)号：US07338774B2

    公开(公告)日：2008-03-04

    申请号：US10074041

    申请日：2002-02-14

    申请人： Hideki Ishihara ,  Tomokazu Yoshida ,  Masatoshi Yamasaki ,  Sachiyo Tada

    发明人： Hideki Ishihara ,  Tomokazu Yoshida ,  Masatoshi Yamasaki ,  Sachiyo Tada

    IPC分类号： C12Q1/48

    CPC分类号： C07K16/40 ,  C12N9/1205 ,  C12Q1/48 ,  C12Q1/485 ,  G01N33/57407 ,  G01N33/57484 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/9121

    摘要： A method for determining the activity of a cell cycle regulatory factor comprising the steps of: preparing a sample for measuring a cyclin-dependent kinase/cyclin complex from living cells; reacting adenosine 5′-O-(3-thiotriphosphate) (ATP-γS) with a substrate for the cyclin-dependent kinase in presence of the sample in order to introduce a monothiophosphate group into a serine or threonine residue of the substrate; labeling the substrate by coupling a labeling fluorophore or a labeling enzyme with a sulfur atom of the introduced monothiophosphate group; measuring the amount of fluorescence from the labeling fluorophore labeling the substrate, or reacting the labeling enzyme labeling the substrate with a substance which generates an optically detectable product by reaction with the labeling enzyme and optically measuring the amount of the generated product; and calculating the activity of the cyclin-dependent kinase from the measured amount of fluorescence or the measured amount of the generated product with reference to a pre-produced reference curve.

    摘要翻译： 一种确定细胞周期调控因子活性的方法，包括以下步骤：制备用于从活细胞测量细胞周期蛋白依赖性激酶/细胞周期蛋白复合物的样品; 在样品存在下使腺苷5'-O-（3-硫代三磷酸酯）（ATP-γS）与用于细胞周期蛋白依赖性激酶的底物反应，以将一硫代磷酸酯基团引入底物的丝氨酸或苏氨酸残基中; 通过将标记的荧光团或标记酶与引入的一硫代磷酸酯基团的硫原子偶联来标记底物; 测量来自标记荧光团的荧光标记基质的荧光量，或使标记基质的标记酶与通过与标记酶反应产生可光学检测的产物并光学测量产生的产物的量的物质反应; 并且根据测量的荧光量或参考预先产生的参考曲线测量产生的产物的量计算细胞周期蛋白依赖性激酶的活性。

    Camera and photographic lens
    9.
    发明授权
    Camera and photographic lens 有权
    相机和摄影镜头

    公开(公告)号：US08774617B2

    公开(公告)日：2014-07-08

    申请号：US13586069

    申请日：2012-08-15

    申请人： Tomokazu Yoshida

    发明人： Tomokazu Yoshida

    IPC分类号： G03B13/22

    CPC分类号： G03B7/095

    摘要： A camera is provided which is adapted to receive a photographic lens having a diaphragm. The camera includes a detection unit configured to execute electric charge accumulation for detecting a defocus amount when the diaphragm is in a state having a first aperture value, and a controller configured to, when causing the diaphragm to perform an aperture reducing operation from the first aperture value to a second aperture value, cause the diaphragm to start the aperture reducing operation without waiting for completion of the electric charge accumulation for detecting the defocus amount by the detection unit.

    摘要翻译： 提供了适于接收具有隔膜的摄影镜头的照相机。 摄像机包括：检测单元，被配置为执行电荷累积，用于当所述隔膜处于具有第一孔径值的状态时检测散焦量;以及控制器，被配置为当使所述隔膜从所述第一孔径执行孔径减小操作时 值到第二光圈值，使得光阑开始光圈减小操作，而不用等待完成由检测单元检测散焦量的电荷累积。