公开(公告)号：US08849024B2

公开(公告)日：2014-09-30

申请号：US12566225

申请日：2009-09-24

申请人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Hideki Ito ,  Satoshi Mori ,  Sato Tsutomu ,  Satoshi Iyama

发明人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Hideki Ito ,  Satoshi Mori ,  Sato Tsutomu ,  Satoshi Iyama

IPC分类号： G06K9/00 ,  G06K9/40 ,  G06K9/46

CPC分类号： G06K9/0014 ,  G06K9/4652 ,  H04N1/6075

摘要： An image processing device can explicitly distinguish cells to be observed from cells other than those to be observed with a simple configuration. To this end, the image processing device includes a color information obtaining part obtaining at least hue from color information of each pixel of a color image, a detecting part detecting a mode value of the hue on a color space, a range setting part setting a predetermined range on the color space including the mode value of the hue detected by the detecting part as a target range, a changing part changing hue of a pixel included in the target range by virtually performing extension on the target range, and an information converting part converting color information of a pixel having hue not included in the target range into color information indicative of an achromatic color.

摘要翻译： 图像处理装置可以用简单的配置，将待观察的细胞与要观察的细胞明确区分开。 为此，图像处理装置包括从彩色图像的每个像素的颜色信息获得至少色调的颜色信息获取部分，检测颜色空间上的色调的模式值的检测部分， 颜色空间上的预定范围，包括由检测部件检测到的色调的模式值作为目标范围，通过在目标范围上虚拟地执行扩展来改变包括在目标范围内的像素的色调的改变部分，以及信息转换部分 将不包括在目标范围中的色调的像素的颜色信息转换成指示无彩色的颜色信息。

公开(公告)号：US20100074508A1

公开(公告)日：2010-03-25

申请号：US12566225

申请日：2009-09-24

申请人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Hideki Ito ,  Satoshi Mori ,  Tsutomu Sato ,  Satoshi Iyama

发明人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Hideki Ito ,  Satoshi Mori ,  Tsutomu Sato ,  Satoshi Iyama

IPC分类号： G06K9/00

CPC分类号： G06K9/0014 ,  G06K9/4652 ,  H04N1/6075

摘要： An image processing device can explicitly distinguish cells to be observed from cells other than those to be observed with a simple configuration. To this end, the image processing device includes a color information obtaining part obtaining at least hue from color information of each pixel of a color image, a detecting part detecting a mode value of the hue on a color space, a range setting part setting a predetermined range on the color space including the mode value of the hue detected by the detecting part as a target range, a changing part changing hue of a pixel included in the target range by virtually performing extension on the target range, and an information converting part converting color information of a pixel having hue not included in the target range into color information indicative of an achromatic color.

摘要翻译： 图像处理装置可以用简单的配置，将待观察的细胞与要观察的细胞明确区分开。 为此，图像处理装置包括从彩色图像的每个像素的颜色信息获得至少色调的颜色信息获取部分，检测颜色空间上的色调的模式值的检测部分， 颜色空间上的预定范围，包括由检测部件检测到的色调的模式值作为目标范围，通过在目标范围上虚拟地执行扩展来改变包括在目标范围内的像素的色调的改变部分，以及信息转换部分 将不包括在目标范围中的色调的像素的颜色信息转换成指示无彩色的颜色信息。

公开(公告)号：US20090141979A1

公开(公告)日：2009-06-04

申请号：US12339925

申请日：2008-12-19

申请人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Satoshi Mori ,  Naoki Watanabe ,  Hideki Ito

发明人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Satoshi Mori ,  Naoki Watanabe ,  Hideki Ito

IPC分类号： G06K9/00

CPC分类号： G06K9/00134

摘要： An inspection apparatus includes an imaging device and an image processing device. The imaging device photographs a specimen and outputs a color image of the specimen to the image processing device. The image processing device subjects the color image of the specimen to negative-positive reversal. After detecting a mode value of the hue the color image having been subjected to negative-positive reversal, the image processing device changes the hue of the color image in accordance with a difference between a boundary value of two predefined hues and the detected mode value. In accordance with the change of the hue, a plurality of target pixels different in the saturation is extracted and the saturation and the intensity of each pixel are changed, or the gradation of each pixel is converted so that the plurality of target pixels becomes most distant from one another in a color space.

摘要翻译： 检查装置包括成像装置和图像处理装置。 成像装置拍摄样本并将样本的彩色图像输出到图像处理装置。 图像处理装置使样本的彩色图像呈负反转。 在检测到彩色图像经受负反转后的色调的模式值之后，图像处理装置根据两个预定色调的边界值与检测到的模式值之间的差异来改变彩色图像的色调。 根据色调的变化，提取饱和度不同的多个目标像素，并且改变每个像素的饱和度和强度，或者转换每个像素的灰度，使得多个目标像素变得最远 在一个色彩空间中彼此之间。

公开(公告)号：US08406514B2

公开(公告)日：2013-03-26

申请号：US12339925

申请日：2008-12-19

申请人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Satoshi Mori ,  Naoki Watanabe ,  Hideki Ito

发明人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Satoshi Mori ,  Naoki Watanabe ,  Hideki Ito

IPC分类号： G06K9/00

CPC分类号： G06K9/00134

摘要： An inspection apparatus includes an imaging device and an image processing device. The imaging device photographs a specimen and outputs a color image of the specimen to the image processing device. The image processing device subjects the color image of the specimen to negative-positive reversal. After detecting a mode value of the hue the color image having been subjected to negative-positive reversal, the image processing device changes the hue of the color image in accordance with a difference between a boundary value of two predefined hues and the detected mode value. In accordance with the change of the hue, a plurality of target pixels different in the saturation is extracted and the saturation and the intensity of each pixel are changed, or the gradation of each pixel is converted so that the plurality of target pixels becomes most distant from one another in a color space.

摘要翻译： 检查装置包括成像装置和图像处理装置。 成像装置拍摄样本并将样本的彩色图像输出到图像处理装置。 图像处理装置使样本的彩色图像呈负反转。 在检测到彩色图像经受负反转后的色调的模式值之后，图像处理装置根据两个预定色调的边界值与检测到的模式值之间的差异来改变彩色图像的色调。 根据色调的变化，提取饱和度不同的多个目标像素，并且改变每个像素的饱和度和强度，或者转换每个像素的灰度，使得多个目标像素变得最远 在一个色彩空间中彼此之间。

公开(公告)号：US20090110273A1

公开(公告)日：2009-04-30

申请号：US12280520

申请日：2007-07-09

申请人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Satoshi Mori ,  Naoki Watanabe ,  Hideki Ito

发明人： Tomotaka Shinoda ,  Toshihiro Mitaka ,  Satoshi Mori ,  Naoki Watanabe ,  Hideki Ito

IPC分类号： G06K9/36 ,  G06K9/00

CPC分类号： H04N1/60 ,  G06T5/009 ,  G06T5/40 ,  G06T2207/10024 ,  G06T2207/30004 ,  H04N1/6075

摘要： An inspection apparatus includes an imaging device and an image processing device. The imaging device photographs a specimen and outputs a color image of the specimen to the image processing device. The image processing device subjects the color image of the specimen to negative-positive reversal. After detecting a mode value of the hue of the color image having been subjected to negative-positive reversal, the image processing device changes the hue of the color image in accordance with a difference between a boundary value of two predefined hues and the detected mode value. In accordance with the change of the hue, a plurality of target pixels different in the saturation is extracted and the saturation and the intensity of each pixel are changed, or the gradation of each pixel is converted so that the plurality of target pixels becomes most distant from one another in a color space.

摘要翻译： 检查装置包括成像装置和图像处理装置。 成像装置拍摄样本并将样本的彩色图像输出到图像处理装置。 图像处理装置使样本的彩色图像呈负反转。 在检测到经过负反转的彩色图像的色相的模式值之后，图像处理装置根据两个预定色调的边界值与检测到的模式值之间的差异来改变彩色图像的色调 。 根据色调的变化，提取饱和度不同的多个目标像素，并且改变每个像素的饱和度和强度，或者转换每个像素的灰度，使得多个目标像素变得最远 在一个色彩空间中彼此之间。

公开(公告)号：US20060128015A1

公开(公告)日：2006-06-15

申请号：US11337698

申请日：2006-01-23

申请人： Toshihiro Mitaka ,  Shinichi Sugimoto

发明人： Toshihiro Mitaka ,  Shinichi Sugimoto

IPC分类号： C12N5/08

CPC分类号： C12N5/067 ,  A61K35/12 ,  C12N2500/90 ,  C12N2503/02 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5067 ,  G01N33/5088

摘要： The present invention provides a process for preparing a transplantable liver tissue, cell colonies suitable for the process, and a process for preparing them. At least about 70 % of the cells in the small hepatocyte-rich colonies of the present invention are small hepatocytes. In particular, each colony comprises about 10 to 30 cells and the number of the small hepatocytes is at least about 70 % based on the total number of the cells in the colony. A process for preparing such small hepatocyte-rich colonies is also disclosed. The present invention also discloses a process for inducing the maturation of the small hepatocyte-rich colonies into a liver tissue and a method of estimating the effect of a drug, particularly the effect thereof connected with normal liver function in vitro, using the cell colonies enriched with the small hepatocytes which have been induced to be matured.

摘要翻译： 本发明提供了一种制备可移植肝组织的方法，适用于该方法的细胞集落及其制备方法。 本发明的富含小肝细胞的集落中至少约70％的细胞是小肝细胞。 特别地，每个菌落包含约10至30个细胞，并且小肝细胞的数目为至少约70％，基于菌落中的细胞总数。 还公开了制备这种小的富含肝细胞的菌落的方法。 本发明还公开了一种诱导小肝细胞富集菌落成肝组织的方法，以及使用富集的细胞集落估计药物效果的方法，特别是其与体外正常肝功能相关的作用的方法 与诱导成熟的小肝细胞。

公开(公告)号：US20070299537A1

公开(公告)日：2007-12-27

申请号：US10579294

申请日：2004-11-12

申请人： Ryo Sudo ,  Kazuo Tanishita ,  Mariko Ikeda ,  Toshihiro Mitaka

发明人： Ryo Sudo ,  Kazuo Tanishita ,  Mariko Ikeda ,  Toshihiro Mitaka

IPC分类号： A61F2/02 ,  A01N1/00 ,  C12N5/08

CPC分类号： C12N5/0671 ,  A61K35/12 ,  A61L27/3839 ,  A61L27/56 ,  C12N5/0062

摘要： Cultured cells flat-cultured on a permeable sheet is stacked on other flat-cultured cells together with the permeable sheet to construct a three-dimensional tissue. The three-dimensional tissue is transplanted into a living body. Alternatively, the three-dimensional tissue is stacked up in an artificial organ device to construct a stacked-up, three-dimensional bioartificial organ module. Colony form of the cultured cell can be controlled by using a microporous sheet as the permeable sheet and controlling positions of pores in the microporous sheet to design the form of the artificial organ.

摘要翻译： 在可渗透片上平铺培养的培养细胞与可渗透片一起堆叠在其他平面培养细胞上以构成三维组织。 将三维组织移植到生物体内。 或者，将三维组织堆叠在人造器官装置中，以构建堆叠的三维生物人造器官模块。 培养细胞的集落形式可以通过使用微孔片作为透过片并控制微孔片中孔的位置来设计人造器官的形状来控制。

公开(公告)号：US20070128720A1

公开(公告)日：2007-06-07

申请号：US11645941

申请日：2006-12-27

申请人： Toshihiro Mitaka ,  Junko Kon

发明人： Toshihiro Mitaka ,  Junko Kon

IPC分类号： C12N5/08 ,  C12N5/06

CPC分类号： G01N33/56966 ,  C12N5/067 ,  C12N2533/80

摘要： A method of culturing small hepatocytes is provided, which method comprises culturing the small hepatocytes in the presence of a carrier of which surface is attached by hyaluronic acid (hyaluronic acid-attached carrier) and/or in the presence of a carrier of which a major component is hyaluronic acid. Additionally, a method of culturing small hepatocytes is also provided, which method comprises culturing the small hepatocytes in the presence of a carrier of which surface is attached by hyaluronic acid and/or in the presence of a carrier of which a major component is hyaluronic acid, and separating cells which adhere to hyaluronic acid from cells which do not adhere to hyaluronic acid.

摘要翻译： 提供培养小肝细胞的方法，该方法包括在其表面附着有透明质酸（透明质酸附着载体）的载体和/或其主要载体的存在下培养小肝细胞 组分是透明质酸。 此外，还提供培养小肝细胞的方法，该方法包括在其表面通过透明质酸附着的载体和/或其主要成分为透明质酸的载体存在下培养小肝细胞 并且从不粘附于透明质酸的细胞中分离粘附于透明质酸的细胞。