Method of modifying isoelectric point of antibody via amino acid substitution in CDR
    2.
    发明授权
    Method of modifying isoelectric point of antibody via amino acid substitution in CDR 有权
    通过CDR中氨基酸取代修饰抗体等电点的方法

    公开(公告)号:US09096651B2

    公开(公告)日:2015-08-04

    申请号:US12679922

    申请日:2008-09-26

    IPC分类号: A61K39/395 C07K16/00

    摘要: Methods are described for modifying the isoelectric point of an antibody while retaining its antigen-binding activity, comprising modifying the charge of at least one exposable amino acid residue on the surface of the complementarity determining region (CDR). The disclosure also provides methods for purifying multispecific antibodies, comprising modifying isoelectric point, and methods for improving the plasma pharmacokinetics of antibodies with a modified isoelectric point. The disclosure further provides antibodies with a modified isoelectric point, pharmaceutical compositions comprising the antibodies as an active ingredient, and methods for producing the antibodies and compositions.

    摘要翻译: 描述了用于修饰抗体的等电点同时保留其抗原结合活性的方法,包括修饰互补决定区(CDR)表面上至少一个可暴露氨基酸残基的电荷。 本公开还提供了用于纯化多特异性抗体的方法,包括修饰等电点,以及用于改进具有修饰的等电点的抗体的血浆药代动力学的方法。 本公开进一步提供具有修饰的等电点的抗体,包含抗体作为活性成分的药物组合物,以及用于产生抗体和组合物的方法。

    Anti-glypican-3 antibody having improved kinetics in plasma
    3.
    发明授权
    Anti-glypican-3 antibody having improved kinetics in plasma 有权
    具有改善血浆动力学的抗磷脂酰肌醇蛋白聚糖-3抗体

    公开(公告)号:US08497355B2

    公开(公告)日:2013-07-30

    申请号:US12733933

    申请日:2008-09-26

    摘要: A method of modulating the plasma half-life of anti-glypican 3 antibody, a pharmaceutical composition comprising as an active ingredient the anti-glypican 3 antibody that has a plasma half-life that has been modulated, a method of preparing the anti-glypican 3 antibody and a pharmaceutical composition comprising the anti-glypican 3 antibody as an active ingredient are provided. Disclosed is a method of modulating the plasma half-life of anti-glypican 3 antibody by modifying an amino acid residue that is exposed on the surface of the anti-glypican 3 antibody; and anti-glypican 3 antibody that has a plasma half-life that has been modulated by amino acid residue modification, a pharmaceutical composition comprising as an active ingredient the anti-glypican 3 antibody, and a method of preparing the anti-glypican 3 antibody and producing a pharmaceutical composition comprising the anti-glypican 3 antibody as an active ingredient.

    摘要翻译: 调节抗磷脂酰肌醇蛋白聚糖3抗体的血浆半衰期的方法,一种药物组合物,其包含具有调节的血浆半衰期的抗磷脂酰肌醇蛋白聚糖3抗体作为活性成分,制备抗磷脂酰肌醇蛋白聚糖的方法 提供了包含抗磷脂酰肌醇蛋白聚糖3抗体作为活性成分的药物组合物。 公开了通过改变暴露于抗磷脂酰肌醇蛋白聚糖3抗体表面的氨基酸残基来调节抗磷脂酰肌醇蛋白聚糖3抗体的血浆半衰期的方法; 和具有通过氨基酸残基修饰调节的血浆半衰期的抗磷脂酰肌醇蛋白聚糖3抗体,包含作为活性成分的磷脂酰肌醇蛋白聚糖3抗体的药物组合物,以及制备抗磷脂酰肌醇蛋白聚糖3抗体的方法和 制备包含抗磷脂酰肌醇蛋白聚糖3抗体作为活性成分的药物组合物。

    ANTI-GLYPICAN-3 ANTIBODY HAVING IMPROVED KINETICS IN PLASMA
    6.
    发明申请
    ANTI-GLYPICAN-3 ANTIBODY HAVING IMPROVED KINETICS IN PLASMA 有权
    具有等离子体改善动力学的抗GLYPICAN-3抗体

    公开(公告)号:US20100239577A1

    公开(公告)日:2010-09-23

    申请号:US12733933

    申请日:2008-09-26

    摘要: A method of modulating the plasma half-life of anti-glypican 3 antibody, a pharmaceutical composition comprising as an active ingredient the anti-glypican 3 antibody that has a plasma half-life that has been modulated, a method of preparing the anti-glypican 3 antibody and a pharmaceutical composition comprising the anti-glypican 3 antibody as an active ingredient are provided. Disclosed is a method of modulating the plasma half-life of anti-glypican 3 antibody by modifying an amino acid residue that is exposed on the surface of the anti-glypican 3 antibody; and anti-glypican 3 antibody that has a plasma half-life that has been modulated by amino acid residue modification, a pharmaceutical composition comprising as an active ingredient the anti-glypican 3 antibody, and a method of preparing the anti-glypican 3 antibody and producing a pharmaceutical composition comprising the anti-glypican 3 antibody as an active ingredient.

    摘要翻译: 调节抗磷脂酰肌醇蛋白聚糖3抗体的血浆半衰期的方法,一种药物组合物,其包含具有调节的血浆半衰期的抗磷脂酰肌醇蛋白聚糖3抗体作为活性成分,制备抗磷脂酰肌醇蛋白聚糖的方法 提供了包含抗磷脂酰肌醇蛋白聚糖3抗体作为活性成分的药物组合物。 公开了通过改变暴露于抗磷脂酰肌醇蛋白聚糖3抗体表面的氨基酸残基来调节抗磷脂酰肌醇蛋白聚糖3抗体的血浆半衰期的方法; 和具有通过氨基酸残基修饰调节的血浆半衰期的抗磷脂酰肌醇蛋白聚糖3抗体,包含作为活性成分的磷脂酰肌醇蛋白聚糖3抗体的药物组合物,以及制备抗磷脂酰肌醇蛋白聚糖3抗体的方法和 制备包含抗磷脂酰肌醇蛋白聚糖3抗体作为活性成分的药物组合物。