公开(公告)号：US5871995A

公开(公告)日：1999-02-16

申请号：US070301

申请日：1991-05-24

申请人： Toshii Iida ,  Toshihiko Kaminuma ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto ,  Jiro Kishimoto ,  Ohji Ifuku ,  Ichiro Kato

发明人： Toshii Iida ,  Toshihiko Kaminuma ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto ,  Jiro Kishimoto ,  Ohji Ifuku ,  Ichiro Kato

IPC分类号： C07K1/107 ,  C12N9/02 ,  C12N15/53 ,  C12N15/60 ,  C12N9/48 ,  C12N9/14 ,  C12N9/78 ,  C12N9/80

CPC分类号： C12N9/0071 ,  C07K1/107 ,  C12Y114/17003 ,  Y10S435/814 ,  Y10S435/815

摘要： A purified enzyme-I is obtained that participates in C-terminal amidation by acting on a peptide C-terminal glycine adduct to form a peptide C-terminal .alpha.-hydroxyglycine adduct. The enzyme has an optimum pH of about 5 to 7, an optimum temperature of 25.degree. to 40.degree. C. and a molecular weight of about 25 kDa or about 36 kDa, and metal ions and ascorbic acid act as a cofactor. A purified enzyme-II is obtained that participates in C-terminal amidation by acting on a peptide C-terminal .alpha.-hydroxyglycine adduct to produce a C-terminal amidated compound. The enzyme has an optimum pH of about 5 to 6, an optimum temperature of 15.degree. to 35.degree. C. and a molecular weight of about 40 kDa or about 43 kDa. Enzyme-I does not act on the peptide C-terminal .alpha.-hydroxyglycine adduct and enzyme-II does not act on the peptide C-terminal glycine adduct. The enzymes may be purified from a biological material such as horse serum by affinity chromatography using a peptide C-terminal glycine adduct as a ligand. The enzymes may also be obtained from host cells transformed with a plasmid containing a cDNA coding for the enzymes. Assay of activity of the enzymes is carried out by measuring adduct (II) or the compound (III) that has been isolated such as by high performance liquid chromatography with the use of an acetonitrile-containing buffer.

摘要翻译： PCT No.PCT / JP90 / 01036 Sec。 371日期1991年5月24日 102（e）日期1991年5月24日PCT提交1990年4月12日PCT公布。 公开号WO91 / 02790 日期1991年3月7日获得通过作用于肽C-末端甘氨酸加合物以形成肽C-末端α-羟基甘氨酸加合物参与C-末端酰胺化的纯化的酶-I。 酶的最佳pH为约5至7，最适温度为25至40℃，分子量为约25kDa或约36kDa，金属离子和抗坏血酸用作辅因子。 获得了通过作用于肽C-末端α-羟基甘氨酸加合物以产生C末端酰胺化合物而参与C-末端酰胺化的纯化的酶-II。 酶的最佳pH为约5至6，最适温度为15-35℃，分子量为约40kDa或约43kDa。 酶-I不对肽C-端α-羟基甘氨酸加合物起作用，酶-II不对肽C-末端甘氨酸加合物起作用。 可以使用肽C-末端甘氨酸加合物作为配体通过亲和层析从生物材料如马血清中纯化酶。 酶也可以从用含有编码酶的cDNA的质粒转化的宿主细胞获得。 酶的活性测定是通过使用含乙腈的缓冲液测定加成物（II）或分离的化合物（III），例如通过高效液相色谱法进行的。

公开(公告)号：US6156555A

公开(公告)日：2000-12-05

申请号：US172120

申请日：1998-10-14

申请人： Toshii Iida ,  Toshihiko Kaminuma ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto ,  Jiro Kishimoto ,  Ohji Ifuku ,  Ichiro Kato

发明人： Toshii Iida ,  Toshihiko Kaminuma ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto ,  Jiro Kishimoto ,  Ohji Ifuku ,  Ichiro Kato

IPC分类号： C07K1/107 ,  C12N9/02 ,  C12N15/53 ,  C12N15/60 ,  C12N9/48 ,  C12N9/14 ,  C12N9/78 ,  C12N9/80 ,  C12N15/00

CPC分类号： C12N9/0071 ,  C07K1/107 ,  C12Y114/17003 ,  Y10S435/814 ,  Y10S435/815

摘要： A purified enzyme-I is obtained that participates in C-terminal amidation by acting on a peptide C-terminal glycine adduct to form a peptide C-terminal .alpha.-hydroxyglycine adduct. The enzyme has an optimum pH of about 5 to 7, an optimum temperature of 25 to 40.degree. C. and a molecular weight of about 25 kDa or about 36 kDa, and metal ions and ascorbic acid act as a cofactor. A purified enzyme-II is obtained that participates in C-terminal amidation by acting on the peptide C-terminal .alpha.-hydroxyglycine adduct to produce a C-terminal amidated compound. The enzyme has an optimum pH of about 5 to 6, an optimum temperature of 15 to 35.degree. C. and a molecular weight of about 40 kDa or about 43 kDa. Enzyme-I does not act on the peptide C-terminal .alpha.-hydroxyglycine adduct and enzyme-II does not act on the peptide C-terminal glycine adduct. The enzymes may be purified from a biological material such as horse serum by affinity chromatography using a peptide C-terminal glycine adduct as a ligand. The enzymes may also be obtained from host cells transformed with a plasmid containing a cDNA coding for the enzymes. Assay of activity of the enzymes is carried out by measuring the C-terminal .alpha.-hydroxyglycine adduct or the C-terminal amidated compound that has been isolated such as by high performance liquid chromatography with the use of an acetonitrile-containing buffer.

摘要翻译： 获得通过作用于肽C-末端甘氨酸加合物以形成肽C末端α-羟基甘氨酸加合物参与C-末端酰胺化的纯化的酶I. 酶的最佳pH为约5至7，最适温度为25至40℃，分子量为约25kDa或约36kDa，金属离子和抗坏血酸用作辅因子。 获得了通过作用于肽C-末端α-羟基甘氨酸加合物参与C-末端酰胺化产生C末端酰胺化合物的纯化的酶-II。 酶的最佳pH为约5至6，最适温度为15至35℃，分子量为约40kDa或约43kDa。 酶-I不对肽C-端α-羟基甘氨酸加合物起作用，酶-II不对肽C-末端甘氨酸加合物起作用。 可以使用肽C-末端甘氨酸加合物作为配体通过亲和层析从生物材料如马血清中纯化酶。 酶也可以从用含有编码酶的cDNA的质粒转化的宿主细胞获得。 酶的活性测定是通过使用含有乙腈的缓冲液，通过高分辨液相色谱法测定已分离的C末端α-羟基甘氨酸加成物或C末端酰胺化合物进行的。

公开(公告)号：US5354675A

公开(公告)日：1994-10-11

申请号：US459829

申请日：1990-01-29

申请人： Toshii Iida ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto

发明人： Toshii Iida ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto

IPC分类号： C07K1/107 ,  C12N9/02 ,  C12P21/00 ,  C12N9/00

CPC分类号： C12N9/0071 ,  C07K1/107 ,  C12Y114/17003

摘要： A C-terminal amidating enzyme composition is disclosed having the ability to amidate the C-terminus of peptides with a C-terminal glycine. The C-terminal amidating enzyme is characterized by having a stable pH range of 5 to 9, metal ions and L-ascorbic acid as cofactors, two active fractions at molecular weights of about 50,000 and about 100,000 as determined from gel filtration, isoelectric points of about 4.5 pH for the active fraction having a molecular weight of about 50,000 and about 6.7 pH for the active fraction having a molecular weight of about 100,000 according to isoelectric point chromatography, and is activated by addition of catalase.

摘要翻译： PCT No.PCT / JP89 / 00521 Sec。 371 1990年1月29日第 102（e）日期1990年1月29日PCT提交1989年5月25日PCT公布。 出版物WO89 / 12096 公开了C端氨酰化酶组合物，其具有用C-末端甘氨酸酰胺化肽​​的C末端的能力。 C末端酰胺化酶的特征在于具有5至9的稳定的pH范围，金属离子和L-抗坏血酸作为辅因子，分子量约50,000和约100,000的活性级分由凝胶过滤确定，等电点 根据等电点色谱法，对于分子量约为50,000的活性级分，对于分子量为约50,000的活性级分和约6.7pH的活性级分为大约4.5的pH，并且通过加入过氧化氢酶来活化。

公开(公告)号：US5612454A

公开(公告)日：1997-03-18

申请号：US776272

申请日：1991-11-29

申请人： Toshihiko Kaminuma ,  Toshii Iida ,  Masahiro Tajima

发明人： Toshihiko Kaminuma ,  Toshii Iida ,  Masahiro Tajima

IPC分类号： C07K1/16 ,  C07K1/36 ,  A61K38/00 ,  A61K38/02 ,  C07K1/00 ,  C07K5/00

CPC分类号： C07K1/16 ,  C07K1/36

摘要： An improved process for purifying a polypeptide using a packing material for reversed phase high performance liquid chromatography is provided. A process for purifying a polypeptide characterized in that an aqueous solution containing polypeptide obtained by pre-treating a polypeptide produced by a wide variety of cells to a predetermined state is adjusted to a specific pH value, to remove impurities, and is then treated with a packing material for reversed phase high performance liquid chromatography.

摘要翻译： PCT No.PCT / JP91 / 00421 Sec。 371日期：1991年11月29日 102（e）1991年11月29日PCT PCT 1991年3月29日PCT。 公开号WO91 / 15502 日期1991年10月17日提供了一种使用反相高效液相色谱法的填充材料纯化多肽的改进方法。 一种纯化多肽的方法，其特征在于，将通过将由多种细胞产生的多肽预处理至预定状态而获得的含有多肽的水溶液调节至特定的pH值以除去杂质，然后用 用于反相高效液相色谱的包装材料。

公开(公告)号：US08937090B2

公开(公告)日：2015-01-20

申请号：US11920053

申请日：2006-04-28

申请人： Mikiko Kaminuma ,  Masaru Suetsugu ,  Toshii Iida ,  Shinji Inomata

发明人： Mikiko Kaminuma ,  Masaru Suetsugu ,  Toshii Iida ,  Shinji Inomata

IPC分类号： A61K31/44 ,  A61K31/135 ,  A61K31/13 ,  A61Q19/00 ,  A61K8/02 ,  A61K8/44 ,  A61K8/49 ,  A61K31/136 ,  A61K31/137 ,  A61K31/131 ,  A61Q1/02 ,  A61Q1/12

CPC分类号： A61Q19/00 ,  A61K8/0212 ,  A61K8/44 ,  A61K8/445 ,  A61K8/447 ,  A61K8/4926 ,  A61K31/131 ,  A61K31/136 ,  A61K31/137 ,  A61Q1/02 ,  A61Q1/12

摘要： The invention provides a parakeratosis inhibitor, pore-shrinking agent, or rough skin preventing/ameliorating agent that has a function such as parakeratosis inhibition, pore shrinkage, or rough skin-inhibition/abatement, poses no safety problems such as sensory irritation, and is very safe, and further provides an external composition for skin to which a compound having the above-mentioned function has been added. The parakeratosis inhibitor, pore-shrinking agent, or rough skin preventing/ameliorating agent comprises one or more compounds selected from the group consisting of β-alanine derivatives and salts thereof. The external composition for skin comprises the one or more compounds selected from the group consisting of β-alanine derivatives and salts thereof as the above-mentioned parakeratosis inhibitor, pore-shrinking agent, or rough skin preventing/ameliorating agent.

摘要翻译： 本发明提供了具有角化不全抑制，孔收缩或粗糙皮肤抑制/消瘦等功能的角化不全抑制剂，孔收缩剂或粗糙皮肤防止/改善剂，不会引起感觉刺激等安全问题，而且 非常安全，并且还提供了已经添加了具有上述功能的化合物的皮肤的外用组合物。 角化不全抑制剂，孔收缩剂或粗糙皮肤防止/改善剂包含一种或多种选自β-丙氨酸衍生物及其盐的化合物。 用于皮肤的外用组合物包含一种或多种选自上述的角化不全抑制剂，毛孔收缩剂或粗糙皮肤防止/改善剂的组合物，其组合物包括β-丙氨酸衍生物及其盐。

公开(公告)号：US20090215893A1

公开(公告)日：2009-08-27

申请号：US11920848

申请日：2006-05-09

申请人： Mikiko Kaminuma ,  Masaru Suetsugu ,  Maki Kaneko ,  Toshii Iida ,  Shinji Inomata

发明人： Mikiko Kaminuma ,  Masaru Suetsugu ,  Maki Kaneko ,  Toshii Iida ,  Shinji Inomata

IPC分类号： A61K31/19

CPC分类号： A61K8/0212 ,  A61K8/44 ,  A61K8/46 ,  A61Q1/02 ,  A61Q19/00

摘要： The invention provides a parakeratosis inhibitor, pore-shrinking agent, or rough skin preventing/amaliorating agent that has a function such as parakeratosis inhibition, pore shrinkage, or rough skin-inhibition/abatement, poses no safety problems such as sensory irritation, and is very safe, and to further provide an external composition for skin to which a compound having the above-mentioned function has been added. The parakeratosis inhibitor agent, pore-shrinking agent, or rough skin preventing/amaliorating agent comprises one, two, or more compounds selected from the group consisting of α-amino acid derivatives and salts thereof. The external composition for skin comprises the one, two, or more compounds selected from the group consisting of α-amino acid derivatives and salts thereof as the above-mentioned parakeratosis inhibitor, pore-shrinking agent, or rough skin preventing/amaliorating agent.

摘要翻译： 本发明提供了具有角化不全抑制，孔收缩或粗糙皮肤抑制/消瘦等功能的角化不全抑制剂，孔收缩剂或粗糙皮肤防止/缓解剂，不会引起感觉刺激等安全性问题，而且 并且为了进一步提供添加了具有上述功能的化合物的皮肤的外用组合物。 角化不全抑制剂，孔收缩剂或粗糙皮肤防止/改善剂包含一种，两种或更多种选自α-氨基酸衍生物及其盐的化合物。 用于皮肤的外用组合物包含一种，两种或更多种选自上述角化不力抑制剂的α-氨基酸衍生物及其盐，孔收缩剂或粗糙皮肤防止/减量剂的化合物。

公开(公告)号：US20080234133A1

公开(公告)日：2008-09-25

申请号：US12011322

申请日：2008-01-25

申请人： Mineyuki Yokoyama ,  Shoko Yamaguchi ,  Toshii Iida ,  Kiyotaka Kojima ,  Koji Kobayashi ,  Osamu Tanaka

发明人： Mineyuki Yokoyama ,  Shoko Yamaguchi ,  Toshii Iida ,  Kiyotaka Kojima ,  Koji Kobayashi ,  Osamu Tanaka

IPC分类号： A01N37/06 ,  A01P21/00

CPC分类号： A01N37/42

摘要： An object of the present invention is to determine novel means of activating plants; more particularly, means of controlling plant growth, such as means of promoting growth, means of controlling dormancy, means of imparting tolerance against stress for plants (dryness, high or low temperatures, osmotic pressure, etc.), and means of preventing aging. The present inventors have found that the above object can be achieved by providing plant activators containing, as an active ingredient, C4-C24 ketol fatty acids, in particular, 9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid.

摘要翻译： 本发明的目的是确定活化植物的新方法; 更具体地，是控制植物生长的手段，例如促进生长的手段，控制休眠的手段，赋予植物胁迫耐受性的手段（干燥，高温或低温，渗透压等）以及防止老化的手段。 本发明人已经发现，上述目的可以通过提供含有作为有效成分的C 4 -C 24脂肪醇脂肪酸的植物活化剂来实现，特别是9 - 羟基-10-氧代-12（Z），15（Z） - 十八碳二烯酸。

公开(公告)号：US08586638B2

公开(公告)日：2013-11-19

申请号：US10580471

申请日：2004-11-22

申请人： Mikiko Kaminuma ,  Masaru Suetsugu ,  Toshii Iida ,  Shinji Inomata ,  Keiko Takada ,  Yuji Katsuta

发明人： Mikiko Kaminuma ,  Masaru Suetsugu ,  Toshii Iida ,  Shinji Inomata ,  Keiko Takada ,  Yuji Katsuta

IPC分类号： A61K47/12

CPC分类号： A61K8/0212 ,  A61K8/44 ,  A61K8/466 ,  A61Q1/02 ,  A61Q19/00

摘要： To provide a parakeratosis inhibitor, pore reducing agent and skin roughness preventing/ameliorating agent that exhibit capabilities of parakeratosis inhibition, pore reduction, skin roughness prevention/amelioration, etc., and further provide a skin preparation for external use having these capabilities. There are provide a parakeratosis inhibitor and a pore reducing agent each comprising at least one compound selected from the group consisting of a glycine derivative, an aminodicarboxylic acid derivative, an acylaminodicarboxylic acid derivative, a pyrrolidinecarboxylic acid derivative, a piperidinecarboxylic acid derivative, a hexamethyleneiminecarboxylic acid, a beta-alanine derivative and salts of these derivatives. Further, there are provided a parakeratosis inhibitor, a pore reducing agent and a skin roughness preventing/ameliorating agent each comprising at least one compound selected from the group consisting of specified glycine derivatives and salts thereof and specified aminosulfuric acid derivatives and salts thereof. Still further, there are provided skin preparations for external use comprising these compounds.

摘要翻译： 提供表现角化不全抑制，减孔，皮肤粗糙度防止/改善等的角化不全抑制剂，减孔剂和皮肤粗糙度防止/改善剂，进一步提供具有这些能力的外用皮肤制剂。 提供了角化不全抑制剂和孔还原剂，其各自包含至少一种选自甘氨酸衍生物，氨基二羧酸衍生物，酰氨基二羧酸衍生物，吡咯烷羧酸衍生物，哌啶羧酸衍生物，六亚甲基亚氨基羧酸 ，β-丙氨酸衍生物和这些衍生物的盐。 此外，提供了分别含有选自特定的甘氨酸衍生物及其盐的化合物和规定的氨基硫酸衍生物及其盐中的至少一种化合物的角化不全抑制剂，减孔剂和皮肤粗糙度防止改善剂。 此外，提供了包含这些化合物的外用皮肤制剂。

公开(公告)号：US20120053243A1

公开(公告)日：2012-03-01

申请号：US13290309

申请日：2011-11-07

申请人： Mikiko Kaminuma ,  Masaru Suetsugu ,  Toshii Iida ,  Shinji Inomata ,  Keiko Takada ,  Yuji Katsuta

发明人： Mikiko Kaminuma ,  Masaru Suetsugu ,  Toshii Iida ,  Shinji Inomata ,  Keiko Takada ,  Yuji Katsuta

IPC分类号： A61K31/197 ,  C07C233/83 ,  C07C235/52 ,  A61K8/44 ,  A61Q19/00 ,  C07C309/19 ,  A61K31/185 ,  A61K8/46 ,  A61P17/00 ,  C07C311/19 ,  C07C309/15

CPC分类号： A61K8/0212 ,  A61K8/44 ,  A61K8/466 ,  A61Q1/02 ,  A61Q19/00

摘要： To provide a parakeratosis inhibitor, pore reducing agent and skin roughness preventing/ameliorating agent that exhibit capabilities of parakeratosis inhibition, pore reduction, skin roughness prevention/amelioration, etc., and further provide a skin preparation for external use having these capabilities. There are provide a parakeratosis inhibitor and a pore reducing agent each comprising at least one compound selected from the group consisting of a glycine derivative, an aminodicarboxylic acid derivative, an acylaminodicarboxylic acid derivative, a pyrrolidinecarboxylic acid derivative, a piperidinecarboxylic acid derivative, a hexamethyleneiminecarboxylic acid, a beta-alanine derivative and salts of these derivatives. Further, there are provided a parakeratosis inhibitor, a pore reducing agent and a skin roughness preventing/ameliorating agent each comprising at least one compound selected from the group consisting of specified glycine derivatives and salts thereof and specified aminosulfuric acid derivatives and salts thereof. Still further, there are provided skin preparations for external use comprising these compounds.

摘要翻译： 提供表现角化不全抑制，减孔，皮肤粗糙度防止/改善等的角化不全抑制剂，减孔剂和皮肤粗糙度防止/改善剂，进一步提供具有这些能力的外用皮肤制剂。 提供了角化不全抑制剂和孔还原剂，其各自包含至少一种选自甘氨酸衍生物，氨基二羧酸衍生物，酰氨基二羧酸衍生物，吡咯烷羧酸衍生物，哌啶羧酸衍生物，六亚甲基亚氨基羧酸 ，β-丙氨酸衍生物和这些衍生物的盐。 此外，提供了分别含有选自特定的甘氨酸衍生物及其盐的化合物和规定的氨基硫酸衍生物及其盐中的至少一种化合物的角化不全抑制剂，减孔剂和皮肤粗糙度防止改善剂。 此外，提供了包含这些化合物的外用皮肤制剂。

公开(公告)号：US20110065794A1

公开(公告)日：2011-03-17

申请号：US12926351

申请日：2010-11-12

申请人： Makoto Tsunenaga ,  Haruhi Iwaki ,  Toshii Iida ,  Mikiko Kaminuma ,  Masaru Suetsugu ,  Keiko Takada ,  Shinji Inomata

发明人： Makoto Tsunenaga ,  Haruhi Iwaki ,  Toshii Iida ,  Mikiko Kaminuma ,  Masaru Suetsugu ,  Keiko Takada ,  Shinji Inomata

IPC分类号： A61K31/197 ,  A61Q19/08

CPC分类号： A61K8/44 ,  A61K8/445 ,  A61Q19/08

摘要： A wrinkle-preventing and -improving composition comprising one or more than one compound selected from the group consisting of α-amino acid derivatives represented by the following general formula (1) and salts thereof: wherein, R1 represents hydrogen atom, CH3 group or CH2OH group, R2 and R3 each independently represent hydrogen atom, alkyl group having 1 to 4 carbons, provided that R2 and R3 cannot be hydrogen atom at the same time, or R2 and R3 together with N atom to which they are bound may form a ring structure having a total carbon number of 4 to 6, in which case said ring structure may optionally contain an oxygen atom as a heteroatom, R4 represents hydrogen atom, alkyl group having 1 to 18 carbons, wherein when R1 and R4 are hydrogen atom, it cannot be that one of R2 and R3 is benzyloxycarbonyl group and the other is hydrogen atom.

摘要翻译： 一种防皱和改善组合物，其包含一种或多于一种选自下列通式（1）表示的α-氨基酸衍生物及其盐的化合物：其中，R 1表示氢原子，CH 3基或CH 2 OH 基团，R 2和R 3各自独立地表示氢原子，具有1至4个碳的烷基，条件是R 2和R 3不能同时为氢原子，或者R 2和R 3与它们所连接的N原子可以形成环 总碳数为4〜6的结构，在这种情况下，所述环结构可任选地含有氧原子作为杂原子，R4表示氢原子，具有1至18个碳原子的烷基，其中当R 1和R 4为氢原子时， 不能是R2和R3之一是苄氧基羰基，另一个是氢原子。