公开(公告)号：US20050164280A1

公开(公告)日：2005-07-28

申请号：US11079947

申请日：2005-03-15

申请人： Toshio Kitamura ,  Sumiyo Morita

发明人： Toshio Kitamura ,  Sumiyo Morita

IPC分类号： A61K38/00 ,  C07K14/475 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/12 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/705 ,  C07K16/28 ,  G01N33/53 ,  G01N33/567

CPC分类号： C07K14/475 ,  A61K38/00

摘要： A gene encoding a novel protein that is homologous to Drosophila TSG was isolated from a cDNA library derived from the AGM region of mouse embryos by using an originally developed cloning method specific to a gene encoding a membrane secretory protein. This gene is useful in developing drugs that regulate hematopoietic stem cell generation, immune and hematopoietic functions, etc.

公开(公告)号：US20090197317A1

公开(公告)日：2009-08-06

申请号：US11940927

申请日：2007-11-15

申请人： Toshio Kitamura ,  Sumiyo Morita

发明人： Toshio Kitamura ,  Sumiyo Morita

IPC分类号： C12N9/96 ,  C07K14/00 ,  C07K16/18

CPC分类号： C07K14/475 ,  A61K38/00

摘要： A gene encoding a novel protein that is homologous to Drosophila TSG was isolated from a cDNA library derived from the AGM region of mouse embryos by using an originally developed cloning method specific to a gene encoding a membrane secretory protein. This gene is useful in developing drugs that regulate hematopoietic stem cell generation, immune and hematopoietic functions, etc.

摘要翻译： 通过使用对编码膜分泌蛋白的基因特异的最初开发的克隆方法，从源自小鼠胚胎的AGM区域的cDNA文库中分离编码与果蝇TSG同源的新蛋白质的基因。 该基因可用于开发调节造血干细胞产生，免疫和造血功能等的药物。

公开(公告)号：US06995009B1

公开(公告)日：2006-02-07

申请号：US10009329

申请日：2000-06-01

申请人： Toshio Kitamura ,  Sumiyo Morita

发明人： Toshio Kitamura ,  Sumiyo Morita

IPC分类号： C12N15/00

CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N2740/10022 ,  C12N2740/10051 ,  C12N2740/10052

摘要： A virus-producing cell sustaining the ability to produce viruses at high titer is successfully constructed by expressing the virus structural gene under the regulation of EF1α promoter. In this virus-producing cell, the virus structural gene is ligated to a selection marker gene via IRES and domains other than the protein coding domain are eliminated from the DNA encoding virus structural proteins. Thus, reduction of the titer due to cell passages can be prevented and emergence of wild type viruses caused by unfavorable recombination of the virus genome can be inhibited.

摘要翻译： 通过在EF1alpha启动子的调节下表达病毒结构基因，成功构建了维持高滴度产生病毒能力的病毒产生细胞。 在该病毒产生细胞中，通过IRES将病毒结构基因连接到选择标记基因，并从编码病毒结构蛋白的DNA中除去蛋白质编码区以外的结构域。 因此，可以防止细胞传代引起的滴度的降低，并且可以抑制由病毒基因组的不利重组引起的野生型病毒的出现。

公开(公告)号：US06890735B2

公开(公告)日：2005-05-10

申请号：US10092925

申请日：2002-03-06

申请人： Toshio Kitamura ,  Sumiyo Morita

发明人： Toshio Kitamura ,  Sumiyo Morita

IPC分类号： A61K38/00 ,  C07K14/475 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/12 ,  C12P21/06 ,  C07H21/04 ,  C07K14/00 ,  C12N15/74 ,  C12N5/92

CPC分类号： C07K14/475 ,  A61K38/00

摘要： A gene encoding a novel protein that is homologous to Drosophila TSG was isolated from a cDNA library derived from the AGM region of mouse embryos by using an originally developed cloning method specific to a gene encoding a membrane secretory protein. This gene is useful in developing drugs that regulate hematopoietic stem cell generation, immune and hematopoietic functions, etc.

摘要翻译： 通过使用对编码膜分泌蛋白的基因特异的最初开发的克隆方法，从源自小鼠胚胎的AGM区域的cDNA文库中分离编码与果蝇TSG同源的新蛋白质的基因。 该基因可用于开发调节造血干细胞产生，免疫和造血功能等的药物。