公开(公告)号：US20060280751A1

公开(公告)日：2006-12-14

申请号：US10546293

申请日：2004-02-18

申请人： Toshio Murakami ,  Hirofumi Higuchi ,  Keiichi Makizumi ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Toshio Murakami ,  Hirofumi Higuchi ,  Keiichi Makizumi ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： A61K39/42 ,  C12Q1/70

CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K16/1063 ,  C07K2317/34

摘要： A method for enhancing efficacy of a monoclonal antibody preparation is provided wherein antigens from patients are tested for their reactivity with said antibody. In accordance with the method of the invention, an amino acid sequence of an expressed protein is deduced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in biopsy from patients and is compared with the previously determined amino acid sequence recognized by said monoclonal antibody preparation to thereby assess fitness of patients for administration of said monoclonal antibody preparation.

摘要翻译： 提供了增强单克隆抗体制剂功效的方法，其中测试来自患者的抗原与其抗体的反应性。 根据本发明的方法，通过从患者的活组织检查中分离和分析靶分子基因而确定的核苷酸序列推导出表达蛋白质的氨基酸序列，并将其与先前确定的所述 从而评估患者适用于所述单克隆抗体制剂的施用。

公开(公告)号：US08332159B2

公开(公告)日：2012-12-11

申请号：US10546293

申请日：2004-02-18

申请人： Toshio Murakami ,  Hirofumi Higuchi ,  Keiichi Makizumi ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Toshio Murakami ,  Hirofumi Higuchi ,  Keiichi Makizumi ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： G06F7/00

CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K16/1063 ,  C07K2317/34

摘要： A method of enhancing the efficacy of a monoclonal antibody preparation wherein antigens from patients are tested for their reactivity with the antibody. An amino acid sequence of an expressed protein is deuced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in a biopsy from a patient. This is compared with the previously determined amino acid sequence recognized by the monoclonal antibody preparation in order to assess the fitness of patients for administration of the monoclonal antibody preparation.

摘要翻译： 一种提高单克隆抗体制剂的功效的方法，其中测试来自患者的抗原与抗体的反应性。 通过从患者的活组织检查中分离和分析靶分子基因而确定的核苷酸序列，从表达的蛋白质的氨基酸序列中去除。 将其与由单克隆抗体制剂识别的先前确定的氨基酸序列进行比较，以评估患者施用单克隆抗体制剂的适合性。

公开(公告)号：US20070065906A1

公开(公告)日：2007-03-22

申请号：US10547802

申请日：2004-02-20

申请人： Satoshi Koyanagi ,  Keishin Sugawara ,  Yoshinobu Miyatsu ,  Toshio Murakami ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Satoshi Koyanagi ,  Keishin Sugawara ,  Yoshinobu Miyatsu ,  Toshio Murakami ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： C12P21/06 ,  C07H21/04 ,  C07K14/435 ,  C07K14/195 ,  C12N1/21 ,  C12N15/74

CPC分类号： C07K14/43531 ,  C12N15/70 ,  C12P21/02

摘要： A heterologous protein free from an inducer and a method for producing said protein are provided. A method for producing a heterologous protein which comprises the step of optionally culturing at low temperature recombinant E. coli cells expressing a heterologous protein under control of a promoter capable of inducing expression through temperature shift and then culturing at high temperature said recombinant E. coli cells in the absence of an inducer to thereby allow for expression of said heterologous protein, or the step of culturing at high temperature said recombinant E. coli cells to thereby simultaneously allow for both cell proliferation and expression of said heterologous protein, and the heterologous protein obtained by said method that is free from an inducer. Such heterologous protein may include a major mite allergen, a secretary macrophage toxin from Actinobacillus pleuropneumoniae and a surface protective antigen (SpaA) of Erysipelothrix rhusiopathiae.

摘要翻译： 提供了不含诱导物的异源蛋白质及其制备方法。 一种产生异源蛋白质的方法，其包括在低温下表达异源蛋白质的重组大肠杆菌细胞的步骤，所述重组大肠杆菌细胞在能够通过温度转移诱导表达并且然后在高温下培养的启动子控制下，所述重组大肠杆菌细胞 在不存在诱导剂从而允许所述异源蛋白质的表达的情况下，或在高温下培养所述重组大肠杆菌细胞从而同时允许所述异源蛋白质的细胞增殖和表达的步骤，获得的异源蛋白质 通过所述方法不含诱导剂。 这种异源蛋白可以包括主要的螨变应原，来自胸膜肺炎放线杆菌的秘密巨噬细胞毒素和Erysipelothrix rhusiopathiae的表面保护性抗原（SpaA）。

公开(公告)号：US07667009B2

公开(公告)日：2010-02-23

申请号：US10555883

申请日：2004-04-14

申请人： Satoshi Koyanagi ,  Kenjiro Kawatsu ,  Toshio Murakami ,  Yoshinobu Miyatsu ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Satoshi Koyanagi ,  Kenjiro Kawatsu ,  Toshio Murakami ,  Yoshinobu Miyatsu ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： A23J1/00

CPC分类号： C07K14/43531 ,  A61K38/00 ,  A61K39/00

摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.

摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。 一种用于纯化通过遗传重组技术获得的改性主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的修饰的主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。

公开(公告)号：US20080113424A1

公开(公告)日：2008-05-15

申请号：US10555883

申请日：2004-04-14

申请人： Satoshi Koyanagi ,  Kenjiro Kawatsu ,  Toshio Murakami ,  Yoshinobu Miyatsu ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Satoshi Koyanagi ,  Kenjiro Kawatsu ,  Toshio Murakami ,  Yoshinobu Miyatsu ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： C07K1/00 ,  C07K16/00

CPC分类号： C07K14/43531 ,  A61K38/00 ,  A61K39/00

摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.

摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。 一种用于纯化通过遗传重组技术获得的改良的主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的改性主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。

公开(公告)号：US08526035B2

公开(公告)日：2013-09-03

申请号：US11984205

申请日：2007-11-14

申请人： Toshihiro Maeda

发明人： Toshihiro Maeda

IPC分类号： G06F3/12

CPC分类号： G06F3/1247 ,  G06F3/1204 ,  G06F3/1229 ,  G06F3/1267 ,  G06F3/1288

摘要： A push-type pull printing system comprising a server and an image forming apparatus, the server sending, to the image forming apparatus, a print job including print data and a print condition instruction command for the print data, and the image forming apparatus executing a print process based on the print job. Here, the image forming apparatus comprises an input receiver operable to receive an input of a print condition, a converter operable to convert the inputted print condition, before being transmitted to the server, to an instruction command in a description language interpretable by the image forming apparatus, and a transmitter operable to transmit the converted instruction command to the server; and the server comprises a job transmitter operable to receive the converted instruction command from and send the print job to the image forming apparatus, the print job including the instruction command as the print condition instruction command.

摘要翻译： 一种包括服务器和图像形成装置的推拉式打印系统，所述服务器向所述图像形成装置发送包括所述打印数据的打印数据和打印条件指令命令的打印作业，并且所述图像形成装置执行 基于打印作业的打印过程。 这里，图像形成装置包括可操作以接收打印条件的输入的输入接收器，可操作以在被发送到服务器之前将输入的打印条件转换为可由图像形成解释的描述语言中的指令命令的转换器 装置和可操作以将转换的指令命令发送到服务器的发射机; 并且所述服务器包括作业发送器，其可操作以从所述图像形成装置接收所述转换的指令命令并向所述图像形成装置发送所述打印作业，所述打印作业包括作为所述打印条件指令命令的所述指令命令。

公开(公告)号：US20080104670A1

公开(公告)日：2008-05-01

申请号：US11808095

申请日：2007-06-06

申请人： Toshihiro Maeda

发明人： Toshihiro Maeda

IPC分类号： G06F15/16 ,  G06F3/12

CPC分类号： G06F3/1229 ,  G06F3/1214 ,  G06F3/1285

摘要： A PC has a data receiving portion to store data in the HDD, and a function ticket generating portion to store in the HDD a function ticket that defines the state of processing executed for the data. Each of a plurality of MFPs has a function ticket acquiring portion to acquire the function ticket, an object processing determining portion to determine executable object processing among processing defined by the function ticket, a data acquiring portion to acquire the data, an object processing executing portion to execute the object processing for the data, a data updating portion to update the data stored in the PC with data for which the object processing has been executed, and a function ticket updating portion to update the state of processing defined by the function ticket stored in the PC with an executed state.

摘要翻译： PC具有用于将数据存储在HDD中的数据接收部分，以及功能单产生部分，用于在HDD中存储定义为数据执行的处理状态的功能票据。 多个MFP中的每一个具有获取功能票的功能票获取部分，用于确定由功能票定义的处理之间的可执行对象处理的对象处理确定部，获取数据的数据获取部，对象处理执行部 执行数据的对象处理，数据更新部分，用于利用已经执行了对象处理的数据更新存储在PC中的数据的数据更新部分和更新由所存储的功能票定义的处理状态的功能票更新部分 在具有执行状态的PC中。

公开(公告)号：US20070177196A1

公开(公告)日：2007-08-02

申请号：US11600877

申请日：2006-11-17

申请人： Toshihiro Maeda

发明人： Toshihiro Maeda

IPC分类号： G06F3/12

CPC分类号： H04N1/00 ,  G03G15/502 ,  G03G15/5087 ,  G03G2215/00109 ,  G03G2215/00113 ,  H04N1/00413 ,  H04N1/00482 ,  H04N1/0097 ,  H04N2201/0039 ,  H04N2201/0094

摘要： In order to easily set a process condition with effective use of a function of each of a plurality of MFPs, an image processing system includes a plurality of MFPs connected to a network. Each of a plurality of MFPs includes a group function information storing unit to store, for each of a plurality of MFPs, function information which defines an individual process that does not allow processed data to be output to any MFP other than that MFP and a shared process that allows processed data to be output to any MFP other than that MFP, a setting screen display unit to read function information of all of a plurality of MFPs to display a setting screen that allows setting of a process condition for performing a process that can be performed in at least one of a plurality of MFPs, a process condition reception unit to receive the process condition, and an output unit to output the process condition to any one of a plurality of MFPs.

摘要翻译： 为了容易地设置具有多个MFP中的每一个的功能的处理条件，图像处理系统包括连接到网络的多个MFP。 多个MFP中的每一个包括组功能信息存储单元，用于为多个MFP中的每一个存储定义不允许处理的数据被输出到除了该MFP以外的任何MFP的单个进程的功能信息，以及共享 将处理后的数据输出到除了该MFP以外的任何MFP的处理，设置画面显示单元，读取多个MFP全部的功能信息，显示设定画面，该设定画面允许设定执行可以 在多个MFP中的至少一个MFP，用于接收处理条件的处理条件接收单元以及将处理条件输出到多个MFP中的任何一个的输出单元中执行。

公开(公告)号：US07952741B2

公开(公告)日：2011-05-31

申请号：US11808095

申请日：2007-06-06

申请人： Toshihiro Maeda

发明人： Toshihiro Maeda

IPC分类号： G06D3/12

CPC分类号： G06F3/1229 ,  G06F3/1214 ,  G06F3/1285

摘要： A PC has a data receiving portion to store data in the HDD, and a function ticket generating portion to store in the HDD a function ticket that defines the state of processing executed for the data. Each of a plurality of MFPs has a function ticket acquiring portion to acquire the function ticket, an object processing determining portion to determine executable object processing among processing defined by the function ticket, a data acquiring portion to acquire the data, an object processing executing portion to execute the object processing for the data, a data updating portion to update the data stored in the PC with data for which the object processing has been executed, and a function ticket updating portion to update the state of processing defined by the function ticket stored in the PC with an executed state.

摘要翻译： PC具有用于将数据存储在HDD中的数据接收部分，以及功能单产生部分，用于在HDD中存储定义为数据执行的处理状态的功能票据。 多个MFP中的每一个具有获取功能票的功能票获取部分，用于确定由功能票定义的处理之间的可执行对象处理的对象处理确定部，获取数据的数据获取部，对象处理执行部 执行数据的对象处理，数据更新部分，用于利用已经执行了对象处理的数据更新存储在PC中的数据的数据更新部分和更新由所存储的功能票定义的处理状态的功能票更新部分 在具有执行状态的PC中。

公开(公告)号：US20060087680A1

公开(公告)日：2006-04-27

申请号：US11011094

申请日：2004-12-15

申请人： Toshihiro Maeda

发明人： Toshihiro Maeda

IPC分类号： G06F15/00

CPC分类号： G06F3/1285 ,  G06F3/1204 ,  G06F3/1227

摘要： In a transmission of a print job to printers, print settings for printers are registered, and setting icons in correspondence to the print settings registered are displayed in a screen of a display device. When a file icon of a document file is detected to be drag-and-dropped onto a setting icon among the setting icons displayed in the screen. Then, a document file in correspondence to the drag-and-dropped file icon and a setting command converted from the print setting corresponded to the setting icon are transmitted as a print job to the specified printer in correspondence to the setting icon.

摘要翻译： 在将打印作业发送到打印机时，登记打印机的打印设置，并且在显示设备的屏幕中显示与登记的打印设置相对应的设置图标。 当在屏幕中显示的设置图标中检测到文档文件的文件图标被拖放到设置图标上时。 然后，对应于设置图标，将与拖放文件图标相对应的文档文件和从对应于设置图标的打印设置转换的设置命令作为打印作业发送到指定的打印机。