公开(公告)号：US20060280751A1

公开(公告)日：2006-12-14

申请号：US10546293

申请日：2004-02-18

申请人： Toshio Murakami ,  Hirofumi Higuchi ,  Keiichi Makizumi ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Toshio Murakami ,  Hirofumi Higuchi ,  Keiichi Makizumi ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： A61K39/42 ,  C12Q1/70

CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K16/1063 ,  C07K2317/34

摘要： A method for enhancing efficacy of a monoclonal antibody preparation is provided wherein antigens from patients are tested for their reactivity with said antibody. In accordance with the method of the invention, an amino acid sequence of an expressed protein is deduced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in biopsy from patients and is compared with the previously determined amino acid sequence recognized by said monoclonal antibody preparation to thereby assess fitness of patients for administration of said monoclonal antibody preparation.

摘要翻译： 提供了增强单克隆抗体制剂功效的方法，其中测试来自患者的抗原与其抗体的反应性。 根据本发明的方法，通过从患者的活组织检查中分离和分析靶分子基因而确定的核苷酸序列推导出表达蛋白质的氨基酸序列，并将其与先前确定的所述 从而评估患者适用于所述单克隆抗体制剂的施用。

公开(公告)号：US08332159B2

公开(公告)日：2012-12-11

申请号：US10546293

申请日：2004-02-18

申请人： Toshio Murakami ,  Hirofumi Higuchi ,  Keiichi Makizumi ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Toshio Murakami ,  Hirofumi Higuchi ,  Keiichi Makizumi ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： G06F7/00

CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K16/1063 ,  C07K2317/34

摘要： A method of enhancing the efficacy of a monoclonal antibody preparation wherein antigens from patients are tested for their reactivity with the antibody. An amino acid sequence of an expressed protein is deuced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in a biopsy from a patient. This is compared with the previously determined amino acid sequence recognized by the monoclonal antibody preparation in order to assess the fitness of patients for administration of the monoclonal antibody preparation.

摘要翻译： 一种提高单克隆抗体制剂的功效的方法，其中测试来自患者的抗原与抗体的反应性。 通过从患者的活组织检查中分离和分析靶分子基因而确定的核苷酸序列，从表达的蛋白质的氨基酸序列中去除。 将其与由单克隆抗体制剂识别的先前确定的氨基酸序列进行比较，以评估患者施用单克隆抗体制剂的适合性。

公开(公告)号：US20070065906A1

公开(公告)日：2007-03-22

申请号：US10547802

申请日：2004-02-20

申请人： Satoshi Koyanagi ,  Keishin Sugawara ,  Yoshinobu Miyatsu ,  Toshio Murakami ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Satoshi Koyanagi ,  Keishin Sugawara ,  Yoshinobu Miyatsu ,  Toshio Murakami ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： C12P21/06 ,  C07H21/04 ,  C07K14/435 ,  C07K14/195 ,  C12N1/21 ,  C12N15/74

CPC分类号： C07K14/43531 ,  C12N15/70 ,  C12P21/02

摘要： A heterologous protein free from an inducer and a method for producing said protein are provided. A method for producing a heterologous protein which comprises the step of optionally culturing at low temperature recombinant E. coli cells expressing a heterologous protein under control of a promoter capable of inducing expression through temperature shift and then culturing at high temperature said recombinant E. coli cells in the absence of an inducer to thereby allow for expression of said heterologous protein, or the step of culturing at high temperature said recombinant E. coli cells to thereby simultaneously allow for both cell proliferation and expression of said heterologous protein, and the heterologous protein obtained by said method that is free from an inducer. Such heterologous protein may include a major mite allergen, a secretary macrophage toxin from Actinobacillus pleuropneumoniae and a surface protective antigen (SpaA) of Erysipelothrix rhusiopathiae.

摘要翻译： 提供了不含诱导物的异源蛋白质及其制备方法。 一种产生异源蛋白质的方法，其包括在低温下表达异源蛋白质的重组大肠杆菌细胞的步骤，所述重组大肠杆菌细胞在能够通过温度转移诱导表达并且然后在高温下培养的启动子控制下，所述重组大肠杆菌细胞 在不存在诱导剂从而允许所述异源蛋白质的表达的情况下，或在高温下培养所述重组大肠杆菌细胞从而同时允许所述异源蛋白质的细胞增殖和表达的步骤，获得的异源蛋白质 通过所述方法不含诱导剂。 这种异源蛋白可以包括主要的螨变应原，来自胸膜肺炎放线杆菌的秘密巨噬细胞毒素和Erysipelothrix rhusiopathiae的表面保护性抗原（SpaA）。

公开(公告)号：US07667009B2

公开(公告)日：2010-02-23

申请号：US10555883

申请日：2004-04-14

申请人： Satoshi Koyanagi ,  Kenjiro Kawatsu ,  Toshio Murakami ,  Yoshinobu Miyatsu ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Satoshi Koyanagi ,  Kenjiro Kawatsu ,  Toshio Murakami ,  Yoshinobu Miyatsu ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： A23J1/00

CPC分类号： C07K14/43531 ,  A61K38/00 ,  A61K39/00

摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.

摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。 一种用于纯化通过遗传重组技术获得的改性主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的修饰的主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。

公开(公告)号：US20080113424A1

公开(公告)日：2008-05-15

申请号：US10555883

申请日：2004-04-14

申请人： Satoshi Koyanagi ,  Kenjiro Kawatsu ,  Toshio Murakami ,  Yoshinobu Miyatsu ,  Toshihiro Maeda ,  Hiroshi Mizokami

发明人： Satoshi Koyanagi ,  Kenjiro Kawatsu ,  Toshio Murakami ,  Yoshinobu Miyatsu ,  Toshihiro Maeda ,  Hiroshi Mizokami

IPC分类号： C07K1/00 ,  C07K16/00

CPC分类号： C07K14/43531 ,  A61K38/00 ,  A61K39/00

摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.

摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。 一种用于纯化通过遗传重组技术获得的改良的主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的改性主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。

公开(公告)号：US08932414B2

公开(公告)日：2015-01-13

申请号：US13634614

申请日：2011-03-22

申请人： Hideo Hata ,  Toshio Murakami ,  Yukihiro Utsumi

发明人： Hideo Hata ,  Toshio Murakami ,  Yukihiro Utsumi

IPC分类号： C22C38/02 ,  C22C38/04 ,  C22C38/06 ,  C21D8/02 ,  C22C38/12 ,  C22C38/14 ,  C22C38/16 ,  C21D8/04 ,  C21D1/20

CPC分类号： C21D8/0226 ,  C21D1/20 ,  C21D8/0447 ,  C21D8/0463 ,  C21D8/0473 ,  C21D2211/001 ,  C21D2211/002 ,  C21D2211/005 ,  C21D2211/008 ,  C22C38/002 ,  C22C38/005 ,  C22C38/02 ,  C22C38/04 ,  C22C38/06 ,  C22C38/08 ,  C22C38/12 ,  C22C38/14 ,  C22C38/16 ,  C22C38/38

摘要： Disclosed is a high-strength steel plate with excellent warm workability that has a component composition comprising, in mass %, 0.05 to 0.4% C, 0.5 to 3% Si+Al, 0.5 to 3% Mn, no more than 0.15% P (not including 0%), and no more than 0.02% S (including 0%), with the remainder comprising iron and impurities, and a composition that includes a total of 45 to 80% martensite and/or bainitic ferrite in terms of the area ratio relative to the entire composition, 5 to 40% polygonal ferrite in terms of the area ratio relative to the entire composition, and 5 to 20% retained austenite in terms of the area ratio relative to the entire composition, wherein the C concentration (CγR) within said residual austenite is in the range of 0.6 mass % to less than 1.0 mass %, and that furthermore may include bainite. In the high-strength steel plate, TRIP effects are achieved to the fullest extent in warm working, and increased ductility over prior steel plates is reliably achieved.

摘要翻译： 公开了一种具有优异的温加工性的高强度钢板，其具有以质量％计含有0.05〜0.4％的C，0.5〜3％的Si + Al，0.5〜3％的Mn，0.15％以下的P（ 不包括0％）和不超过0.02％的S（包括0％），剩余部分包括铁和杂质，以及包括总共45-80％的马氏体和/或贝氏体铁素体的组合物的面积 相对于整个组合物的比率，相对于组合物的面积比为5〜40％的多边形铁素体，相对于组合物的面积比为5〜20％的残留奥氏体，其中C浓度 ）在0.6质量％以下且小于1.0质量％的范围内，并且还可以包括贝氏体。 在高强度钢板中，TRIP效果在温暖加工中达到最大程度，并且可靠地实现了比现有钢板更高的延展性。

公开(公告)号：US20140271331A1

公开(公告)日：2014-09-18

申请号：US14238599

申请日：2012-08-15

申请人： Toshio Murakami ,  Elijah Kakiuchi ,  Hideo Hata ,  Naoki Mizuta ,  Tatsuya Asai

发明人： Toshio Murakami ,  Elijah Kakiuchi ,  Hideo Hata ,  Naoki Mizuta ,  Tatsuya Asai

IPC分类号： C22C38/38 ,  C22C38/14 ,  C22C38/12 ,  C22C38/00 ,  C22C38/06 ,  C22C38/04 ,  C22C38/02 ,  C22C38/16 ,  C22C38/08

CPC分类号： C22C38/38 ,  B21D22/208 ,  C21D8/0226 ,  C21D8/0236 ,  C21D9/46 ,  C21D2211/001 ,  C21D2211/002 ,  C21D2211/008 ,  C22C38/001 ,  C22C38/002 ,  C22C38/02 ,  C22C38/04 ,  C22C38/06 ,  C22C38/08 ,  C22C38/12 ,  C22C38/14 ,  C22C38/16

摘要： This high-strength steel plate has a component composition including, by mass %, C: 0.02-0.3%, Si: 1-3%, Mn: 1.8-3%, P: 0.1% or less, S: 0.01% or less, Al: 0.001-0.1%, N: 0.002-0.03%, the rest consisting of iron and impurities. Said steel plate has a microstructure including, in terms of area ratio relative to the entire microstructure, each of the following phases: bainitic ferrite: 50-85%; retained γ; 3% or greater; martensite+the aforementioned retained γ; 10-45%; and ferrite: 5-40%. The C concentration (CγR) in the aforementioned retained austenite is 0.3-1.2 mass %, part or all of the N in the aforementioned component composition is solid solution N, and the amount of said solid solution N is 30-100 ppm.

摘要翻译： 该高强度钢板的组成成分按质量％计含有C：0.02-0.3％，Si：1-3％，Mn：1.8-3％，P：0.1％以下，S：0.01％以下 ，Al：0.001-0.1％，N：0.002-0.03％，其余由铁和杂质组成。 所述钢板具有包括相对于整个微观结构的面积比的以下各相的微结构：贝氏体铁素体：50-85％; 保留γ; 3％以上; 马氏体+上述保留的γ; 10-45％; 铁素体：5-40％。 上述残留奥氏体中的C浓度（CγR）为0.3〜1.2质量％，上述成分组成中的N的一部分或全部为固溶N，所述固溶N的量为30〜100ppm。

公开(公告)号：US20140161659A1

公开(公告)日：2014-06-12

申请号：US14237286

申请日：2012-08-15

申请人： Toshio Murakami ,  Masao Kinefuchi ,  Hideo Hata

发明人： Toshio Murakami ,  Masao Kinefuchi ,  Hideo Hata

IPC分类号： C22C38/28 ,  C22C38/16 ,  C22C38/14 ,  C22C38/00 ,  C22C38/08 ,  C22C38/06 ,  C22C38/04 ,  C22C38/02 ,  C22C38/24 ,  C22C38/12

CPC分类号： C22C38/28 ,  C21D8/0226 ,  C21D9/46 ,  C21D2211/002 ,  C21D2211/004 ,  C21D2211/005 ,  C22C38/001 ,  C22C38/02 ,  C22C38/04 ,  C22C38/06 ,  C22C38/08 ,  C22C38/12 ,  C22C38/14 ,  C22C38/16 ,  C22C38/24

摘要： This high-strength hot-rolled steel plate contains specific amounts of C, Si, Mn, Al, V and also Ti and/or Nb so as to fulfill C−12(V/51+Ti/48+Nb/93)>0.03, and the rest consists of iron and unavoidable impurities. Ferrite is the main microstructure, the remaining microstructure is one or more selected from the group consisting of bainite, martensite and retained austenite, wherein the average particle diameter of precipitated carbides (the total content of V, Ti and Nb is 0.02% or greater) in the ferrite is less than 6 nm.

摘要翻译： 该高强度热轧钢板含有特定量的C，Si，Mn，Al，V以及Ti和/或Nb，以满足C-12（V / 51 + Ti / 48 + Nb / 93）> 0.03，其余由铁和不可避免的杂质组成。 铁素体是主要的微结构，剩余的微结构是选自贝氏体，马氏体和残余奥氏体中的一种或多种，​​其中沉淀碳化物的平均粒径（V，Ti和Nb的总含量为0.02％以上） 在铁素体中小于6nm。

公开(公告)号：US20130236350A1

公开(公告)日：2013-09-12

申请号：US13988382

申请日：2011-11-16

申请人： Elijah Kakiuchi ,  Toshio Murakami ,  Hideo Hata ,  Tatsuya Asai ,  Naoki Mizuta

发明人： Elijah Kakiuchi ,  Toshio Murakami ,  Hideo Hata ,  Tatsuya Asai ,  Naoki Mizuta

IPC分类号： C22C38/38 ,  C22C38/14 ,  C22C38/12 ,  B21D11/22 ,  C22C38/06 ,  C22C38/04 ,  C22C38/02 ,  C22C38/00 ,  C22C38/16 ,  C22C38/08

摘要： A high-strength steel sheet has a chemical composition including 0.05% to 0.3% of C, 1% to 3% of Si, 0.5% to 3% of Mn, 0% to 0.1% of P, 0.001% to 0.1% of Al, and 0.002% to 0.03% of N, in mass percent; further includes iron and impurities; and has a structure including 50% to 90% of bainitic ferrite, 5% to 20% of retained austenite (γR), a total of 10% to 50% of martensite and the retained austenite, and 0% to 40% of polygonal ferrite, in area percent based on the entire structure. The retained austenite has a carbon content (CγR) of 0.5% to 1.2% by mass, an average equivalent circle diameter of 0.2 to 2 μm, and an average aspect ratio (maximum diameter/minimum diameter) of less than 3.0. The high-strength steel sheet excels both in elongation and deep drawability while having a strength of 980 MPa or more.

摘要翻译： 高强度钢板具有C：0.05〜0.3％，Si：1〜3％，Mn：0.5〜3％，P：0〜0.1％，Al：0.001〜0.1％ ，以质量％计，N：0.002〜0.03％ 进一步包括铁和杂质; 并且具有包括50％至90％的贝氏体铁素体，5％至20％的残留奥氏体（γR），总共10％至5​​0％的马氏体和残余奥氏体以及0％至40％的多边形铁素体 ，占整个结构的面积百分比。 残留奥氏体的碳含量（CgammaR）为0.5〜1.2质量％，平均当量圆直径为0.2〜2μm，平均纵横比（最大直径/最小直径）小于3.0。 高强度钢板的强度为980MPa以上，伸长率和深冲性均优异。

公开(公告)号：US08343288B2

公开(公告)日：2013-01-01

申请号：US12919159

申请日：2009-03-06

申请人： Toshio Murakami ,  Akira Ibano

发明人： Toshio Murakami ,  Akira Ibano

IPC分类号： C22C38/44

CPC分类号： C22C38/04 ,  C21D1/25 ,  C21D8/0236 ,  C21D2211/004 ,  C21D2211/005 ,  C21D2211/008 ,  C22C38/06

摘要： Provided are the following cold-rolled steel sheets: 1) a cold-rolled steel sheet having higher stretch flangeability than conventional steels; 2) a cold-rolled steel sheet having a higher balance between elongation and stretch flangeability than conventional steels; and 3) a cold-rolled steel sheet heightened in all of yield stress, elongation, and stretch flangeability. The cold-rolled steel sheets are characterized by containing 0.03-0.30 mass % carbon, up to 3.0 mass % (including 0 mass %) silicon, 0.1-5.0 mass % manganese, up to 0.1 mass % phosphorus, less than 0.01 mass % sulfur, up to 0.01 mass % nitrogen, and 0.01-1.00 mass % aluminum and having a structure which comprises tempered martensite in an amount of 50% or more (including 100%) in terms of areal proportion and in which the remainder is ferrite. The steel sheets are further characterized in that at least one of the following structural factors has been regulated: the proportions of cementite particles and of the ferrite grains in the tempered martensite and the dislocation density in all structures.

摘要翻译： 提供以下冷轧钢板：1）具有比常规钢更高的拉伸凸缘性的冷轧钢板; 2）与常规钢相比，延伸率和拉伸凸缘性之间的平衡比较高的冷轧钢板; 和3）在所有屈服应力，伸长率和拉伸凸缘性方面提高的冷轧钢板。 冷轧钢板的特征在于，含有0.03-0.30质量％的碳，至多3.0质量％（包括0质量％）的硅，0.1-5.0质量％的锰，至多0.1质量％的磷，小于0.01质量％的硫 ，至多0.01质量％的氮和0.01〜1.00质量％的铝，并且具有以面积比例计为50％以上（包括100％）量的回火马氏体，其余为铁素体的结构。 钢板的特征还在于，以下结构因素中的至少一个已被调节：回火马氏体中的渗碳体颗粒和铁素体晶粒的比例以及所有结构中的位错密度。