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1.发明申请公开(公告)号：US20080069826A1
公开(公告)日：2008-03-20
申请号：US11869267
申请日：2007-10-09
申请人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
发明人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
CPC分类号： C07K16/18 , A61K38/00 , A61K2039/505 , A61K2039/53 , C07K14/47 , C07K14/4702 , C12N2799/021
摘要： A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.
摘要翻译： 使用鸡ard平滑肌细胞培养系统，差异显示法和减法杂交法分离参与维持平滑肌分化的cDNA片段。 使用获得的cDNA序列作为查询，检索Helix研究所的cDNA序列（日本专利申请2000-118776），从而得到新的基因“C-NT2RP3001495”。 由该基因编码的蛋白质具有参与N末端结构域中的蛋白质相互作用的两个WW结构域。 证据表明，该蛋白质与其他蛋白质结合，从而调节细胞内信号转导，基因表达等，从而参与维持平滑肌细胞的分化。 该蛋白质和调节其表达的化合物在开发用于维持平滑肌细胞分化异常的各种疾病的药物中显着有用。
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2.发明授权公开(公告)号：US08110662B2
公开(公告)日：2012-02-07
申请号：US11869267
申请日：2007-10-09
申请人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
发明人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
CPC分类号： C07K16/18 , A61K38/00 , A61K2039/505 , A61K2039/53 , C07K14/47 , C07K14/4702 , C12N2799/021
摘要： A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.
摘要翻译： 使用鸡ard平滑肌细胞培养系统，差异显示法和减法杂交法分离参与维持平滑肌分化的cDNA片段。 使用获得的cDNA序列作为查询，检索Helix研究所的cDNA序列（日本专利申请2000-118776），从而得到新的基因“C-NT2RP3001495”。 由该基因编码的蛋白质具有参与N末端结构域中的蛋白质相互作用的两个WW结构域。 证据表明，该蛋白质与其他蛋白质结合，从而调节细胞内信号转导，基因表达等，从而参与维持平滑肌细胞的分化。 该蛋白质和调节其表达的化合物在开发用于维持平滑肌细胞分化异常的各种疾病的药物中显着有用。
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3.发明授权公开(公告)号：US06908748B2
公开(公告)日：2005-06-21
申请号：US10058518
申请日：2002-01-28
申请人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
发明人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
CPC分类号： C07K16/18 , A61K38/00 , A61K2039/505 , A61K2039/53 , C07K14/47 , C07K14/4702 , C12N2799/021
摘要： A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.
摘要翻译： 使用鸡ard平滑肌细胞培养系统，差异显示法和减法杂交法分离参与维持平滑肌分化的cDNA片段。 使用获得的cDNA序列作为查询，检索Helix研究所的cDNA序列（日本专利申请2000-118776），从而得到新的基因“C-NT2RP3001495”。 由该基因编码的蛋白质具有参与N末端结构域中的蛋白质相互作用的两个WW结构域。 证据表明，该蛋白质与其他蛋白质结合，从而调节细胞内信号转导，基因表达等，从而参与维持平滑肌细胞的分化。 该蛋白质和调节其表达的化合物在开发用于维持平滑肌细胞分化异常的各种疾病的药物中显着有用。
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4.发明授权公开(公告)号：US07279558B2
公开(公告)日：2007-10-09
申请号：US11051410
申请日：2005-02-04
申请人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
发明人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
CPC分类号： C07K16/18 , A61K38/00 , A61K2039/505 , A61K2039/53 , C07K14/47 , C07K14/4702 , C12N2799/021
摘要： A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.
摘要翻译： 使用鸡ard平滑肌细胞培养系统，差异显示法和减法杂交法分离参与维持平滑肌分化的cDNA片段。 使用获得的cDNA序列作为查询，检索Helix研究所的cDNA序列（日本专利申请2000-118776），从而得到新的基因“C-NT2RP3001495”。 由该基因编码的蛋白质具有参与N末端结构域中的蛋白质相互作用的两个WW结构域。 证据表明，该蛋白质与其他蛋白质结合，从而调节细胞内信号转导，基因表达等，从而参与维持平滑肌细胞的分化。 该蛋白质和调节其表达的化合物在开发用于维持平滑肌细胞分化异常的各种疾病的药物中显着有用。
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5.发明申请公开(公告)号：US20050164934A1
公开(公告)日：2005-07-28
申请号：US11051410
申请日：2005-02-04
申请人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
发明人： Toshio Ota , Takao Isogai , Tetsuo Nishikawa , Koji Hayashi , Kaoru Otsuka , Jun-Ichi Yamamoto , Shizuko Ishii , Tomoyasu Sugiyama , Ai Wakamatsu , Keiichi Nagai , Tetsuji Otsuki , Shin-Ichi Funahashi , Shoji Miyata , Kenji Sobue , Kenichiro Hayashi
IPC分类号： A61K38/00 , C07K14/47 , C12Q1/68 , C07K14/705 , C07K16/28
CPC分类号： C07K16/18 , A61K38/00 , A61K2039/505 , A61K2039/53 , C07K14/47 , C07K14/4702 , C12N2799/021
摘要： A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.
摘要翻译： 使用鸡ard平滑肌细胞培养系统，差异显示法和减法杂交法分离参与维持平滑肌分化的cDNA片段。 使用获得的cDNA序列作为查询，检索Helix研究所的cDNA序列（日本专利申请2000-118776），从而得到新的基因“C-NT2RP3001495”。 由该基因编码的蛋白质具有参与N末端结构域中的蛋白质相互作用的两个WW结构域。 证据表明，该蛋白质与其他蛋白质结合，从而调节细胞内信号转导，基因表达等，从而参与维持平滑肌细胞的分化。 该蛋白质和调节其表达的化合物在开发用于维持平滑肌细胞分化异常的各种疾病的药物中显着有用。
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公开(公告)号：US07829083B2
公开(公告)日：2010-11-09
申请号：US12013346
申请日：2008-01-11
IPC分类号： A61K39/395 , C12N5/10 , C12N5/12 , G01N33/53
CPC分类号： C07K14/705
摘要： A novel gene apparently encoding a transmembrane glycoprotein has been successfully isolated by constructing a cDNA library of 4 kb or above in size from mRNA expressed in human adult brain and analyzing the structures of the cDNAs contained within said library by the shotgun method. The novel gene shows brain-specific expression and the protein encoded by said gene has a typical PDZ binding motif.
摘要翻译： 通过构建人大脑中表达的mRNA的大小为4kb或以上的cDNA文库，并通过霰弹枪法分析所述文库中所含的cDNA的结构，已经成功地分离了显然编码跨膜糖蛋白的新基因。 该新基因显示脑特异性表达，由该基因编码的蛋白具有典型的PDZ结合基序。
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公开(公告)号：US07001728B1
公开(公告)日：2006-02-21
申请号：US09502698
申请日：2000-02-11
申请人： Shin-Ichi Funahashi , Shoji Miyata
发明人： Shin-Ichi Funahashi , Shoji Miyata
IPC分类号： G01N33/53 , C07K14/705
CPC分类号： C07K16/18 , A61K38/00 , C07K14/4718
摘要： While analyzing changes in gene expression by TNFα in umbilical vascular endothelial cells, a gene showing enhanced expression due to stimulation with TNFα was isolated. After screening with this gene as a probe, a gene encoding a protein was isolated. Analysis of the protein encoded by this isolated gene revealed that this novel protein has never been reported and has a PDZ domain in its molecule that plays an important role in protein—protein interactions.
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公开(公告)号：US06852836B1
公开(公告)日：2005-02-08
申请号：US09831846
申请日：1999-11-18
IPC分类号： C07K14/705 , C12N1/21 , C12N15/12 , C12P21/08 , C07K14/47
CPC分类号： C07K14/705
摘要： A novel gene apparently encoding a transmembrane glycoprotein has been successfully isolated by constructing a cDNA library of 4 kb or above in size from mRNA expressed in human adult brain and analyzing the structures of cDNAs contained within said library by the shotgun method. The novel gene shows brain-specific expression and the protein encoded by said gene has a typical PDZ protein binding motif.
摘要翻译： 通过构建人大脑中表达的mRNA的大小为4kb或以上的cDNA文库，并通过霰弹枪法分析所述文库中所含的cDNA的结构，已经成功地分离了显然编码跨膜糖蛋白的新基因。 该新基因显示脑特异性表达，由该基因编码的蛋白具有典型的PDZ蛋白结合基序。
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公开(公告)号：US20090258022A1
公开(公告)日：2009-10-15
申请号：US12013346
申请日：2008-01-11
IPC分类号： A61K39/395 , C07K16/18 , C12N5/06 , G01N33/566 , A61P37/02
CPC分类号： C07K14/705
摘要： A novel gene apparently encoding a transmembrane glycoprotein has been successfully isolated by constructing a cDNA library of 4 kb or above in size from mRNA expressed in human adult brain and analyzing the structures of the cDNAs contained within said library by the shotgun method. The novel gene shows brain-specific expression and the protein encoded by said gene has a typical PDZ binding motif.
摘要翻译： 通过构建人大脑中表达的mRNA的大小为4kb或以上的cDNA文库，并通过霰弹枪法分析所述文库中所含的cDNA的结构，已经成功地分离了显然编码跨膜糖蛋白的新基因。 该新基因显示脑特异性表达，由该基因编码的蛋白具有典型的PDZ结合基序。
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公开(公告)号：US20060141517A1
公开(公告)日：2006-06-29
申请号：US11311454
申请日：2005-12-19
申请人： Shin-Ichi Funahashi , Shoji Miyata
发明人： Shin-Ichi Funahashi , Shoji Miyata
IPC分类号： C07K14/705 , C12Q1/68 , C07H21/04 , C12P21/06
CPC分类号： C07K16/18 , A61K38/00 , C07K14/4718
摘要： While analyzing changes in gene expression by TNFα in human umbilical vascular endothelial cells, a gene showing enhanced expression due to stimulation with TNFα was isolated. After screening with this gene as a probe, a gene encoding a protein was isolated. Analysis of the protein encoded by this isolated gene revealed that this novel protein has never been reported and has a PDZ domain in its molecule that plays an important role in protein-protein interactions.
摘要翻译： 在分析人脐血管内皮细胞中TNFα的基因表达变化的同时，分离出由TNFα刺激引起的表达增强的基因。 用该基因筛选后，分离出编码蛋白质的基因。 由该分离基因编码的蛋白质的分析显示，这种新型蛋白质从未被报道，并且在其分子中具有在蛋白质 - 蛋白质相互作用中起重要作用的PDZ结构域。
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