公开(公告)号：US12129465B2

公开(公告)日：2024-10-29

申请号：US17563271

申请日：2021-12-28

Applicant: UNIVERSITY OF WASHINGTON ,  FRED HUTCHINSON CANCER RESEARCH CENTER

Inventor： Daniel Ellis ,  Neil King ,  Jesse Bloom ,  Tyler Starr ,  Allison Greaney

IPC: C07K14/165 ,  A61K39/215 ,  C12N15/11 ,  C12N15/64 ,  C12Q1/6811

CPC classification number: C12N15/11 ,  A61K39/215 ,  C07K14/165 ,  C12N15/64 ,  C12Q1/6811

Abstract: Provided herein are compositions and methods comprising mutated coronavirus “S” spike proteins or receptor binding domains thereof that have an increased expression level, yield and stability compared to its corresponding native or wild-type coronavirus spike protein under the same expression, culture or storage conditions. These mutated spike proteins can be used for generating a protein-based vaccine against one or more coronaviruses.

公开(公告)号：US20240165218A1

公开(公告)日：2024-05-23

申请号：US18359154

申请日：2023-07-26

Applicant: University of Washington

Inventor： Neil King ,  David Baker ,  Lance Stewart ,  Brooke Fiala ,  Daniel Ellis ,  Lauren Carter ,  Rashmi Ravichandran ,  George Ueda ,  Jorge Fallas ,  Una Natterman

IPC: A61K39/12 ,  C12N15/86

CPC classification number: A61K39/12 ,  C12N15/86 ,  B82Y5/00

Abstract: The present disclosure provides nanostructures and nanostructure-based vaccines. Some nanostructures of the present disclosure display antigens capable of eliciting immune responses to infectious agents such as bacteria, viruses, and pathogens. Some vaccines of the present disclosure are useful for preventing or decreasing the severity of infection with an infectious agent, including. for example and without limitation, lyme disease, pertussis, herpes virus, orthomyxovirus, paramyxovirus, pneumovirus, filovirus. flavivirus, reovirus, retrovirus, meningococcus, or malaria. The antigens may be attached to the core of the nanostructure either non-covalently or covalently, including as a fusion protein or by other means disclosed herein. Multimeric antigens may optionally be displayed along a symmetry axis of the nanostructure. Also provided are proteins and nucleic acid molecules encoding such proteins. vaccine compositions, and methods of administration.

公开(公告)号：US11485759B2

公开(公告)日：2022-11-01

申请号：US16427493

申请日：2019-05-31

Applicant: UNIVERSITY OF WASHINGTON

Inventor： David Baker ,  Jacob B. Bale ,  Neil P. King ,  William H. Sheffler ,  Daniel Ellis

IPC: C07K14/00

Abstract: Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.

公开(公告)号：US09630994B2

公开(公告)日：2017-04-25

申请号：US14930792

申请日：2015-11-03

Applicant: UNIVERSITY OF WASHINGTON

Inventor： David Baker ,  Jacob B. Bale ,  Neil P. King ,  William H. Sheffler ,  Daniel Ellis

IPC: A61K38/16 ,  C07K14/00

CPC classification number: C07K14/00

Abstract: Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.

公开(公告)号：US10351603B2

公开(公告)日：2019-07-16

申请号：US15490351

申请日：2017-04-18

Applicant: UNIVERSITY OF WASHINGTON

Inventor： David Baker ,  Jacob B. Bale ,  Neil P. King ,  William H. Sheffler ,  Daniel Ellis

IPC: C07K14/00

Abstract: Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.

公开(公告)号：US11771755B2

公开(公告)日：2023-10-03

申请号：US16971278

申请日：2019-02-28

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Neil King ,  David Baker ,  Lance Stewart ,  Brooke Fiala ,  Daniel Ellis ,  Lauren Carter ,  Rashmi Ravichandran ,  George Ueda ,  Jorge Fallas ,  Una Nattermann

IPC: A61K39/12 ,  C12N15/86 ,  B82Y5/00 ,  B82Y40/00

CPC classification number: A61K39/12 ,  C12N15/86 ,  B82Y5/00 ,  B82Y40/00 ,  C12N2710/16134 ,  C12N2710/16234 ,  C12N2760/00034

Abstract: Nanostructures and nanostructure-based vaccines that display antigens capable of eliciting immune responses to infectious agents such as bacteria, viruses, and pathogens are provided. Some vaccines are useful for preventing or decreasing the severity of infection with an infectious agent, including, for example and without limitation, lyme disease, pertussis, herpes virus, orthomyxovirus, paramyxovirus, pneumovirus, filovirus, flavivirus, reovirus, retrovirus, meningococcus, or malaria. The antigens may be attached to the core of the nanostructure either non-covalently or covalently, including as a fusion protein or by other means. Multimeric antigens may optionally be displayed along a symmetry axis of the nanostructure. Also provided are proteins and nucleic acid molecules encoding such proteins, vaccine compositions, and methods of administration.