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    Antibiotic rubradirin and method of making same
    5.
    发明授权
    Antibiotic rubradirin and method of making same 失效
    抗生素rubradirin及其制备方法

    公开(公告)号：US3335057A

    公开(公告)日：1967-08-08

    申请号：US29265763

    申请日：1963-07-03

    申请人： UPJOHN CO

    发明人： JOHNSON LE ROY E ,  SMITH ROBERT M ,  MEYER CURTIS E ,  OWEN STANLEY P

    IPC分类号： C07G11/00 ,  C12P1/06 ,  C12P17/18

    CPC分类号： A61K31/70 ,  C07G11/00 ,  C12P1/06 ,  C12P17/188 ,  C12R1/465 ,  Y10S435/886

    摘要： An antibiotic rubradirin gives a red colour in acid solution and a green colour in alkaline solution, it is effective against gram positive and negative bacteria; it is soluble in ethyl acetate, 1-butanol, methyl ethyl ketone, methylene chloride, methanol and benzene and relatively insoluble in water; it has the following elemental analysis: C=57.31%, H=4.69%, N=5.25% and O=31.36%; molecular weight 1026\sB12; ultra-violet absorption spectrum (Fig. 2) showing maxima at 264 mm , a = 28.48; 337 mm , a = 34.73 and 515 mm , a = 1.58; and infra-red absorption spectrum as in Fig. 1. Rubradirin is produced by cultivating Streptomyces achromogenes var. rubradiris NRRL 3061 (N.C.I.B. 9516) in an aqueous nutrient medium containing an assimiliable source of carbon and nitrogen under aerobic conditions. The temperature is 18 DEG to 40 DEG C., pH 6 to 8 and duration of cultivation 2 to 10 days. Rubradirin may be recovered from the clarified broth by (a) extraction or (b) adsorption. Extraction from a broth of pH 3.2, by means of a solvent such as methylene chloride, may be followed by precipitation by a non-solvent such as Skellysolve B. Adsorption may be on strongly basic anion exchange resins, e.g. Amberlite IRS-400 or on silicic acid, alumina or "Florisil" (Registered Trade Mark), followed by elution with a solvent. Further purification may be by counter-current distribution, e.g. in the solvent system Skellysolve B, acetone and water (5 : 5 : 1). Rubradirin forms salts with organic and inorganic bases, e.g. alkali metal, alkaline earth metal and amine bases.

    摘要翻译： 抗生素rubradirin在酸性溶液中呈现红色，在碱性溶液中呈绿色，对革兰氏阳性菌和阴性菌有效; 它溶于乙酸乙酯，1-丁醇，甲基乙基酮，二氯甲烷，甲醇和苯，相对不溶于水; 它具有以下元素分析：C = 57.31％，H = 4.69％，N = 5.25％，O = 31.36％。 分子量1026 \ sB12; 紫外吸收光谱（图2）显示最大值为264 mm，a = 28.48; 337 mm，a = 34.73和515 mm，a = 1.58; 和红外吸收光谱如图1所示。 鲁布列宁是通过培养链霉菌（Streptomyces achromogenes var。 Rubradiris NRRL 3061（N.C.I.B.9516）在含氧营养培养基中，在需氧条件下含有可同化的碳源和氮源。 温度为18〜40℃，pH6〜8，培养时间为2〜10天。 可以通过（a）提取或（b）吸附从澄清的肉汤中回收鲁布丁灵。 通过溶剂如二氯甲烷从pH3.2的肉汤中提取，之后可以通过非溶剂如Skellysolve B沉淀。吸附可以在强碱性阴离子交换树脂上，例如， Amberlite IRS-400或硅酸，氧化铝或“Florisil”（注册商标），然后用溶剂洗脱。 进一步的纯化可以是逆流分布，例如 在溶剂体系Skellysolve B，丙酮和水（5：5：1）中。 Rubradirin与有机和无机碱形成盐，例如 碱金属，碱土金属和胺基。

    Antibiotic bluensin and method of production
    7.
    发明授权
    Antibiotic bluensin and method of production 失效
    抗生素蓝蛋白和生产方法

    公开(公告)号：US3880827A

    公开(公告)日：1975-04-29

    申请号：US12973961

    申请日：1961-08-07

    申请人： UPJOHN CO

    发明人： EBLE THOMAS E ,  JOHNSON LE ROY E

    IPC分类号： C07G11/00 ,  C12P19/54 ,  C07G3/00 ,  C07C129/18

    CPC分类号： C12P19/54 ,  C07G11/00 ,  Y10S435/886

    摘要： This invention relates to a novel composition of matter and to a process for the production thereof. More particularly, this invention relates to a new compound, bluensin, and to a process for the production thereof and its use as antibacterial agent.

    摘要翻译： 本发明涉及一种新型的物质组合物及其生产方法。 更具体地说，本发明涉及新化合物，蓝青霉素及其生产方法及其作为抗菌剂的用途。