-
公开(公告)号：US20230265524A1
公开(公告)日：2023-08-24
申请号：US18008053
申请日：2021-06-03
Applicant: UNIVERSITY HEALTH NETWORK
Inventor： Daniel DINIZ DE CARVALHO , Parinaz MEHDIPOUR , Sajid MARHON
IPC: C12Q1/6886 , A61K31/706 , A61P35/00
CPC classification number: C12Q1/6886 , A61K31/706 , A61P35/00 , C12Q2600/106 , C12Q2600/158 , C12Q2600/154
Abstract: There is described herein a method of assessing a subject's responsiveness to cancer therapy, comprising: providing a sample from the subject comprising cancers cells or suspected cancer cells; measuring or estimating the expression levels of inverted repeats (IR) Alus in the cells; and determining that the subject would be responsive to 5 cancer therapy if the subject cells exhibit expression levels of inverted repeats (IR) Alus with reference to expression levels in control samples.
-
公开(公告)号：US20230227821A1
公开(公告)日：2023-07-20
申请号：US17916981
申请日：2021-04-07
Applicant: UNIVERSITY HEALTH NETWORK
Inventor： Daniel DINIZ DE CARVALHO , Housheng HE , Ilias ETTAYEBI , Fraser SOARES , Helen LOO YAU
IPC: C12N15/113 , C12N5/0783 , A61K35/17 , A61P35/00
CPC classification number: C12N15/113 , C12N5/0636 , A61K35/17 , A61P35/00 , C12N2310/20
Abstract: Methods of increasing T cell effector function in a T cell population are provided that involve inhibiting one or more genetic subunits of the SAGA (Spt-Ada-Gcn5-acetyltransferase) gene regulation complex in the T cell population. Also provided are methods of using such T cell populations in the treatment of cancer patients.
-
公开(公告)号：US20250006375A1
公开(公告)日：2025-01-02
申请号：US18820166
申请日：2024-08-29
Applicant: University Health Network
Abstract: This is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
-
Patent Agency Ranking
公开(公告)号：US20230203473A1
公开(公告)日：2023-06-29
申请号：US18061273
申请日：2022-12-02
Applicant: University Health Network , Sinai Health System
Inventor： Daniel DINIZ DE CARVALHO , Shu Yi SHEN , Rajat SINGHANIA , Scott Victor BRATMAN , Ankur RAVINARAYANA CHAKRAVARTHY
IPC: C12N15/10 , C12Q1/6806 , C12Q1/6804 , G01N33/53
CPC classification number: C12N15/1003 , C12N15/1093 , C12N15/10 , C12Q1/6806 , C12Q1/6804 , G01N33/5308 , C12Q1/6886
Abstract: There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
-
公开(公告)号：US20220251665A1
公开(公告)日：2022-08-11
申请号：US17668314
申请日：2022-02-09
Applicant: UNIVERSITY HEALTH NETWORK , SINAI HEALTH SYSTEM
Inventor： Daniel DINIZ DE CARVALHO , Scott Victor BRATMAN , Rajat SINGHANIA , Ankur RAVINARAYANA CHAKRAVARTHY , Shu Yi SHEN
IPC: C12Q1/6886 , C12Q1/6827
Abstract: There is described herein a method of detecting the presence of DNA from cancer cells in a subject comprising: providing a sample of cell-free DNA from a subject; subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated, then optionally denaturing the sample; capturing cell-free methylated DNA using a binder selective for methylated polynucleotides; sequencing the captured cell-free methylated DNA; comparing the sequences of the captured cell-free methylated DNA to control cell-free methylated DNAs sequences from healthy and cancerous individuals and from individuals with distinct cancer types and subtypes; identifying the presence of DNA from cancer cells if there is a statistically significant similarity between one or more sequences of the captured cell-free methylated DNA and cell-free methylated DNAs sequences from cancerous individuals.
-
公开(公告)号：US20230287384A1
公开(公告)日：2023-09-14
申请号：US18059370
申请日：2022-11-28
Applicant: University Health Network , Sinai Health System
Inventor： Daniel DINIZ DE CARVALHO , Shu Yi SHEN , Rajat SINGHANIA
IPC: C12N15/10 , C12Q1/6806 , C12Q1/6804 , G01N33/53
CPC classification number: C12N15/1003 , C12N15/10 , C12N15/1093 , C12Q1/6804 , C12Q1/6806 , G01N33/5308 , C12Q1/6886
Abstract: There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
-
公开(公告)号：US20230212690A1
公开(公告)日：2023-07-06
申请号：US18067661
申请日：2022-12-16
Applicant: UNIVERSITY HEALTH NETWORK
IPC: C12Q1/6886 , G16B30/00
CPC classification number: C12Q1/6886 , G16B30/00 , C12Q2600/154 , C12Q2600/156
Abstract: In an aspect, there is provided a method of detecting the presence of ctDNA from cancer cells in a subject comprising: (a) providing a sample of cell-free DNA from a subject; (b) subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; (c) optionally adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated, then further optionally denaturing the sample; (d) capturing cell-free methylated DNA using a binder selective for methylated polynucleotides; (e) sequencing the captured cell-free methylated DNA; (f) comparing the sequences of the captured cell-free methylated DNA to control cell-free methylated DNAs sequences from healthy and cancerous individuals; (g) identifying the presence of DNA from cancer cells if there is a statistically significant similarity between one or more sequences of the captured cell-free methylated DNA and cell-free methylated DNAs sequences from cancerous individuals; wherein in at least one of the capturing step, the comparing step or the identifying step, the subject cell-free methylated DNA is limited to a sub-population according to a fragment length metric.
-
公开(公告)号：US20230024827A1
公开(公告)日：2023-01-26
申请号：US17736570
申请日：2022-05-04
Applicant: UNIVERSITY HEALTH NETWORK , VAN ANDEL RESEARCH INSTITUTE
Inventor： Samantha L. WILSON , Shu Yi SHEN , Daniel DINIZ DE CARVALHO , Michael M. HOFFMAN , Timothy J. TRICHE
IPC: C12Q1/6858
Abstract: There is described herein, a method of capturing and analyzing cell-free methylated DNA in a sample. The method involves subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA. A predetermined amount of control synthetic DNA fragments are added to the sample. The control synthetic DNA fragments each have a known nucleic acid sequence that does not align to a target genome sequence, and at least some of the control synthetic DNA fragments are methylated. The sample is denatured, and cell-free methylated DNA and the control synthetic DNA fragments are captured using a binder selective for methylated polynucleotides. The captured DNA is amplified and sequenced.
-
-
-
-
-
-
-
Search Results
8
records
(took 0.017 seconds)
