公开(公告)号：US12072274B2

公开(公告)日：2024-08-27

申请号：US17276717

申请日：2019-09-16

申请人： University of Southampton ,  The University of Western Australia

发明人： Hywel Morgan ,  Daniel Spencer ,  Timothy John Jay Inglis ,  Teagan Paton ,  Kieran Mulroney

IPC分类号： G01N15/12 ,  C12Q1/18 ,  G01N15/02 ,  G01N15/0227 ,  G01N15/10 ,  G01N15/1031

CPC分类号： G01N15/12 ,  C12Q1/18 ,  G01N15/0227 ,  G01N15/1023 ,  G01N15/1031 ,  G01N2015/0294 ,  G01N2015/1006 ,  G01N2015/1029 ,  G01N2015/103

摘要： A method of antimicrobial susceptibility testing comprises: preparing samples of microorganisms suspended in an electrolyte, comprising a first sample of the microorganisms unexposed to antimicrobial agents and a second sample of the microorganisms exposed to an antimicrobial agent; passing the first sample through an impedance flow cytometer to obtain a first impedance signal representing one or more components of impedance values of the unexposed microorganisms; passing the second sample through the impedance flow cytometer to obtain a second impedance signal representing one or more components of the impedance values of the exposed microorganisms; comparing the first impedance signal and the second impedance signal; and determining a susceptibility of the microorganisms to the antimicrobial agent based on any differences between the first impedance signal and second impedance signal.

公开(公告)号：US20210292807A1

公开(公告)日：2021-09-23

申请号：US16500710

申请日：2018-04-06

申请人： The University of Western Australia

发明人： Timothy John Jay Inglis ,  Kieran Mulroney ,  Jarrad Hall

IPC分类号： C12Q1/18 ,  G01N15/14

摘要： The present invention relates to methods for the qualitative and quantitative determination of the susceptibility of microbial organisms such as bacteria to antimicrobial agents such as antibiotics. For example, the present invention provides a method of determining the susceptibility of a unicellular or multicellular microorganism to an antimicrobial agent by acoustic flow cytometry. The method comprising exposing cells of the microorganism to at least one antimicrobial agent, wherein said cells of the microorganism are in and/or derived from a sample obtained from a subject having or suspected of having an infection with said microorganism; measuring effect of said at least one antimicrobial agent on cellular morphology of said cells of the microorganism by acoustic flow cytometry; and determining susceptibility of the microorganism to the at least one antimicrobial agent from the acoustic flow cytometry data output.