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    APTAMER-BASED LATERAL FLOW ASSAY AND ASSOCIATED METHODS
    1.
    发明申请
    APTAMER-BASED LATERAL FLOW ASSAY AND ASSOCIATED METHODS 有权
    基于APTAMER的侧流测定及相关方法

    公开(公告)号：US20140011193A1

    公开(公告)日：2014-01-09

    申请号：US13894161

    申请日：2013-05-14

    申请人： University of Utah Research Foundation

    发明人： Jennifer M. Heemstra

    IPC分类号： G01N33/53

    CPC分类号： G01N33/5308 ,  C12Q1/6816 ,  G01N33/558 ,  G01N33/946 ,  C12Q2525/205

    摘要： Methods, assays, and products for the detection of analytes in a sample are provided. In one aspect, for example, a device for detecting an analyte in a sample can include a fluid transfer membrane further including a sample input region operable to receive a liquid sample, a reagent region including a first split aptamer segment, a second split aptamer segment, and a detection marker, where the first and second split aptamers are operable to ligate in the presence of the analyte. The detection marker is operable to bind to the second split aptamer. The device can further include a test region having an immobilized binding reagent operable to bind to the first split aptamer segment such that the detection marker is held in the test region when the first split aptamer segment is ligated to the second split aptamer segment due to the analyte being present in the sample.

    摘要翻译： 提供了用于检测样品中分析物的方法，测定和产品。 在一个方面，例如，用于检测样品中的分析物的装置可以包括流体转移膜，其还包括可操作以接收液体样品的样品输入区域，包括第一裂解适体区段的试剂区域，第二裂解适体区段 和检测标记，其中第一和第二裂解适体可操作以在分析物的存在下连接。 检测标记可操作以结合第二分裂适体。 该装置可以进一步包括具有固定的结合试剂的测试区域，其可操作地结合第一分裂适体区段，使得当第一分裂适体区段连接到第二分裂适体区段时，由于检测区域 分析物存在于样品中。

    Small molecule-dependent split aptamer ligation
    4.
    发明授权
    Small molecule-dependent split aptamer ligation 有权
    小分子依赖性分裂适体连接

    公开(公告)号：US09279805B2

    公开(公告)日：2016-03-08

    申请号：US14288240

    申请日：2014-05-27

    申请人： University of Utah Research Foundation

    发明人： Jennifer M. Heemstra

    IPC分类号： C12N15/115 ,  G01N33/53 ,  G01N33/94 ,  G01N33/74

    CPC分类号： G01N33/9486 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/10 ,  G01N33/53 ,  G01N33/5308 ,  G01N33/743 ,  G01N33/94 ,  G01N33/946 ,  G01N2400/00

    摘要： Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.

    摘要翻译： 提供了用于检测小分子的方法，测定和产物。 在一个方面，例如，检测样品中的小分子的方法可以包括将具有与其偶联的第一反应性基团的DNA裂解适体的第一半一起反应，具有第二反应性基团的DNA裂解适体的第二半 其中DNA裂解适体对小分子是选择性的，以及含有小分子的样品。 上半部和第二半部分与小分子结合，第一反应基团和第二反应基团反应形成上半部和下半部分的适体连接产物。 该方法还可以包括测定适体连接产物以检测样品中的小分子存在。

    Multi-sequence capture system
    6.
    发明授权
    Multi-sequence capture system 有权

    公开(公告)号：US10465242B2

    公开(公告)日：2019-11-05

    申请号：US15650832

    申请日：2017-07-14

    申请人： University of Utah Research Foundation

    发明人： John Phillips ,  William M. Hanson ,  Jennifer M. Heemstra

    IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6816

    摘要： Systems, devices, and methods for capturing single source-specific biological material from a multi-source aggregate of biological material are disclosed and discussed. A capture system is generated using reversible chain-blocking to make capture substrates having substrate-linked populations of capture molecules specific for molecules of interest. Incubating such capture substrates in the presence of only a single source of biological material facilitates the association of molecules of interest from the same source. Capture substrate-specific barcode sequences coupled to the capture molecules allow multisource aggregate processing and subsequent grouping to retain the source-specific information following downstream processing.

    MULTI-SEQUENCE CAPTURE SYSTEM
    7.
    发明申请
    MULTI-SEQUENCE CAPTURE SYSTEM 审中-公开

    公开(公告)号：US20180057874A1

    公开(公告)日：2018-03-01

    申请号：US15650832

    申请日：2017-07-14

    申请人： University of Utah Research Foundation

    发明人： John Phillips ,  William M. Hanson ,  Jennifer M. Heemstra

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6876 ,  C12Q2531/113 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/519

    摘要： Systems, devices, and methods for capturing single source-specific biological material from a multi-source aggregate of biological material are disclosed and discussed. A capture system is generated using reversible chain-blocking to make capture substrates having substrate-linked populations of capture molecules specific for molecules of interest. Incubating such capture substrates in the presence of only a single source of biological material facilitates the association of molecules of interest from the same source. Capture substrate-specific barcode sequences coupled to the capture molecules allow multisource aggregate processing and subsequent grouping to retain the source-specific information following downstream processing.

    SMALL MOLECULE-DEPENDENT SPLIT APTAMER LIGATION
    10.
    发明申请
    SMALL MOLECULE-DEPENDENT SPLIT APTAMER LIGATION 有权
    小分子依赖分离APTAMER LIGATION

    公开(公告)号：US20150072352A1

    公开(公告)日：2015-03-12

    申请号：US14288240

    申请日：2014-05-27

    申请人： University of Utah Research Foundation

    发明人： Jennifer M. Heemstra

    IPC分类号： G01N33/53

    CPC分类号： G01N33/9486 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/10 ,  G01N33/53 ,  G01N33/5308 ,  G01N33/743 ,  G01N33/94 ,  G01N33/946 ,  G01N2400/00

    摘要： Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.

    摘要翻译： 提供了用于检测小分子的方法，测定和产物。 在一个方面，例如，检测样品中的小分子的方法可以包括将具有与其偶联的第一反应性基团的DNA裂解适体的第一半一起反应，具有第二反应性基团的DNA裂解适体的第二半 其中DNA裂解适体对小分子是选择性的，以及含有小分子的样品。 上半部和第二半部分与小分子结合，第一反应基团和第二反应基团反应形成上半部和下半部分的适体连接产物。 该方法还可以包括测定适体连接产物以检测样品中的小分子存在。