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公开(公告)号：US09157917B2
公开(公告)日：2015-10-13
申请号：US13883067
申请日：2011-11-04
申请人： Lukas Wick , Urs Spitz , Christophe Weymuth , Günter Schabert , Thomas Mayer , Alexander Bayer
发明人： Lukas Wick , Urs Spitz , Christophe Weymuth , Günter Schabert , Thomas Mayer , Alexander Bayer
IPC分类号： C07D221/18 , C09B67/08 , G01N33/58 , C09B57/00 , C09B67/44 , C12Q1/04 , C12Q1/10 , C12Q1/34 , C12Q1/44
CPC分类号： G01N33/583 , C09B57/00 , C09B67/0083 , C12Q1/04 , C12Q1/10 , C12Q1/34 , C12Q1/44 , G01N2333/938
摘要： A novel type of dye systems comprises a selection of 10H-indolo[1,2-a]indole compounds (henceforth abbreviated as IO compounds) and (5H,7H)-indolo[1,2-a]quinoline compounds (henceforth abbreviated as IQ compounds) showing a solvatochromic effect and exhibiting strong fluorescence in a variety of materials such as polypropylene, polyethylene, oils, various solvents, emulsions. Also disclosed are various methods how the IO/IQ compounds can be administered, especially how they can be produced and administered in situ from a precursor, responding to external stimuli such as enzyme activity, temperature and so forth. The response of a precursor to external stimuli can also be used to determine the presence or absence of such stimuli.
摘要翻译： 一种新型染料体系包括10H-吲哚并[1,2-a]吲哚化合物（以下简称为IO化合物）和（5H，7H） - 吲哚并[1,2-a]喹啉化合物（以下简称为 IQ化合物），显示出溶剂化色素效应，并在各种材料如聚丙烯，聚乙烯，油，各种溶剂，乳液中表现出强烈的荧光。 还公开了如何施用IO / IQ化合物的各种方法，特别是如何从前体产生和原位施用，对外部刺激如酶活性，温度等进行响应。 前体对外部刺激的反应也可以用于确定这种刺激的存在或不存在。
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公开(公告)号：US20120058503A1
公开(公告)日：2012-03-08
申请号：US13266707
申请日：2010-05-06
申请人： Urs Spitz , Lukas Wick , Alexander Bayer , Christophe Weymuth , Günter Schabert
发明人： Urs Spitz , Lukas Wick , Alexander Bayer , Christophe Weymuth , Günter Schabert
CPC分类号： C07D209/36 , C07D209/70 , C07D487/04
摘要： A novel indicator platform comprises a plurality of 1H-lndol-3-yl indicator compounds that are capable of converting to a signalophore compound in response to an external stimulus. In one class of indicator compounds, the resulting signalophores are 2-benzylideneindoline compounds that are formed by an intermolecular Aldol-type process; in a further class of indicator compounds, the resulting signalophores are 10H-indolo[1,2-a]indole compounds that are formed by an intramolecular Aldol-type process. The indicators can be used in a wide array of applications relating, for example, to biological systems or optical data storage.
摘要翻译： 一种新颖的指示剂平台包含能够响应外部刺激转化成信号载体化合物的多个1H-吲哚-3-基指示剂化合物。 在一类指示剂化合物中，所得到的信号聚合物是通过分子间Aldol型方法形成的2-亚苄基二氢吲哚化合物; 在另一类指示剂化合物中，所得到的信号聚合物是通过分子内Aldol型方法形成的10H-吲哚并[1,2-a]吲哚化合物。 这些指标可用于涉及例如生物系统或光学数据存储的广泛应用。
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公开(公告)号：US08940909B2
公开(公告)日：2015-01-27
申请号：US13266707
申请日：2010-05-06
申请人： Urs Spitz , Lukas Wick , Alexander Bayer , Christophe Weymuth , Günter Schabert
发明人： Urs Spitz , Lukas Wick , Alexander Bayer , Christophe Weymuth , Günter Schabert
IPC分类号： C07D209/70 , C07D209/36 , C07D487/04
CPC分类号： C07D209/36 , C07D209/70 , C07D487/04
摘要： A novel indicator platform comprises a plurality of 1H-lndol-3-yl indicator compounds that are capable of converting to a signalophore compound in response to an external stimulus. In one class of indicator compounds, the resulting signalophores are 2-benzylideneindoline compounds that are formed by an intermolecular Aldol-type process; in a further class of indicator compounds, the resulting signalophores are 10H-indolo[1,2-a]indole compounds that are formed by an intramolecular Aldol-type process. The indicators can be used in a wide array of applications relating, for example, to biological systems or optical data storage.
摘要翻译： 一种新颖的指示剂平台包含能够响应外部刺激转化成信号载体化合物的多个1H-吲哚-3-基指示剂化合物。 在一类指示剂化合物中，所得到的信号聚合物是通过分子间Aldol型方法形成的2-亚苄基二氢吲哚化合物; 在另一类指示剂化合物中，所得到的信号聚合物是通过分子内Aldol型方法形成的10H-吲哚并[1,2-a]吲哚化合物。 这些指标可用于涉及例如生物系统或光学数据存储的广泛应用。
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公开(公告)号：US20140377783A1
公开(公告)日：2014-12-25
申请号：US13883067
申请日：2011-11-04
申请人： Lukas Wick , Urs Spitz , Christophe Weymuth , Günter Schabert , Thomas Mayer , Alexander Bayer
发明人： Lukas Wick , Urs Spitz , Christophe Weymuth , Günter Schabert , Thomas Mayer , Alexander Bayer
CPC分类号： G01N33/583 , C09B57/00 , C09B67/0083 , C12Q1/04 , C12Q1/10 , C12Q1/34 , C12Q1/44 , G01N2333/938
摘要： A novel type of dye systems comprises a selection of 10H-indolo[1,2-a]indole compounds (henceforth abbreviated as IO compounds) and (5H,7H)-indolo[1,2-a]quinoline compounds (henceforth abbreviated as IQ compounds) showing a solvatochromic effect and exhibiting strong fluorescence in a variety of materials such as polypropylene, polyethylene, oils, various solvents, emulsions. Also disclosed are various methods how the IO/IQ compounds can be administered, especially how they can be produced and administered in situ from a precursor, responding to external stimuli such as enzyme activity, temperature and so forth. The response of a precursor to external stimuli can also be used to determine the presence or absence of such stimuli.
摘要翻译： 一种新型染料体系包括10H-吲哚并[1,2-a]吲哚化合物（以下简称为IO化合物）和（5H，7H） - 吲哚并[1,2-a]喹啉化合物（以下简称为 IQ化合物），显示出溶剂化色素效应，并在各种材料如聚丙烯，聚乙烯，油，各种溶剂，乳液中表现出强烈的荧光。 还公开了如何施用IO / IQ化合物的各种方法，特别是如何从前体产生和原位施用，对外部刺激如酶活性，温度等进行响应。 前体对外部刺激的反应也可以用于确定这种刺激的存在或不存在。
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公开(公告)号：US20140212905A1
公开(公告)日：2014-07-31
申请号：US14123861
申请日：2012-06-08
申请人： Urs Spitz , Lukas Wick , Günter Schabert
发明人： Urs Spitz , Lukas Wick , Günter Schabert
IPC分类号： C12Q1/34
摘要： A method for detection of beta-D-glucuronidase activity in a sample that comprises the steps of (i) providing a glucuronic acid ester compound of the general formula (I) wherein R1 is a C1-4 alkyl group, OR2 is a dye moiety, which is liberated after cleavage of the glycosidic bond; (ii) contacting said glucuronic acid ester compound with a material of said sample exhibiting hydrolytic activity towards glucuronic acid esters, thereby removing R1 and thus forming a sample containing an indicator compound suitable for the detection and/or measurement of beta-D-glucuronidase activity, and (iii) using said indicator to perform an assay requiring detection or measurement of beta-D-glucuronidase activity.
摘要翻译： 一种检测样品中β-D-葡糖醛酸糖苷酶活性的方法，包括以下步骤：（i）提供通式（I）的葡萄糖醛酸酯化合物，其中R 1是C 1-4烷基，OR 2是染料部分 ，其在糖苷键断裂后释放; （ii）使所述葡糖醛酸酯化合物与所述样品对葡萄糖醛酸酯显示出水解活性的物质接触，从而除去R1，从而形成含有适于检测和/或测量β-D-葡糖醛酸糖苷酶活性的指示剂化合物的样品 ，和（iii）使用所述指示剂进行需要检测或测量β-D-葡糖苷酸酶活性的测定。
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公开(公告)号：US09127303B2
公开(公告)日：2015-09-08
申请号：US14123861
申请日：2012-06-08
申请人： Urs Spitz , Lukas Wick , Gunter Schabert
发明人： Urs Spitz , Lukas Wick , Gunter Schabert
摘要： A method for detection of beta-D-glucuronidase activity in a sample that comprises the steps of (i) providing a glucuronic acid ester compound of the general formula (I) wherein R1 is a C1-4 alkyl group, OR2 is a dye moiety, which is liberated after cleavage of the glycosidic bond; (ii) contacting said glucuronic acid ester compound with a material of said sample exhibiting hydrolytic activity towards glucuronic acid esters, thereby removing R1 and thus forming a sample containing an indicator compound suitable for the detection and/or measurement of beta-D-glucuronidase activity, and (iii) using said indicator to perform an assay requiring detection or measurement of beta-D-glucuronidase activity.
摘要翻译： 一种检测样品中β-D-葡糖醛酸糖苷酶活性的方法，包括以下步骤：（i）提供通式（I）的葡萄糖醛酸酯化合物，其中R 1是C 1-4烷基，OR 2是染料部分 ，其在糖苷键断裂后释放; （ii）使所述葡糖醛酸酯化合物与所述样品对葡萄糖醛酸酯显示出水解活性的物质接触，从而除去R1，从而形成含有适于检测和/或测量β-D-葡糖醛酸糖苷酶活性的指示剂化合物的样品 ，和（iii）使用所述指示剂进行需要检测或测量β-D-葡糖苷酸酶活性的测定。
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公开(公告)号：US20100291591A1
公开(公告)日：2010-11-18
申请号：US12740929
申请日：2008-11-04
申请人： Lukas Wick , Guenter Schabert , Urs Spitz
发明人： Lukas Wick , Guenter Schabert , Urs Spitz
IPC分类号： G01N33/573
CPC分类号： C12Q1/045
摘要： Plating media contains enzyme substrates (ES) used to detect enzyme activity associated with a target organism and that include a binding motif (BM) that serves to bind detectable enzymes (DE) (also called marker enzymes in this disclosure). A signalogenic substructure (SG) produces detectable signals and an enzyme labile group (ELG) is labile to the action of a marker enzyme and it typically includes the binding motif. The enzyme labile group links to the signalogenic substructure via a labile bond (LB) which is cleaved by the action of the marker enzyme separating the signalogenic substructure and the enzyme labile group. In some embodiments a linker is inserted between the signalogenic substructure and the enzyme labile group. Presence of the enzyme and thus the organism is detected by presence of a fluorogenic, precipitate that can be detected in single colonies.
摘要翻译： 电镀培养基包含用于检测与靶生物相关的酶活性的酶底物（ES），其包括用于结合可检测酶（DE）（本公开中也称为标记酶）的结合基序（BM）。 信号发生子结构（SG）产生可检测的信号，酶不稳定基团（ELG）对标记酶的作用是不稳定的，并且其典型地包括结合基序。 酶不稳定基团通过不稳定键（LB）连接到信号产生亚结构，其通过分离信号基因亚基和酶不稳定基团的标记酶的作用而被切割。 在一些实施方案中，将接头插入在信号产生亚结构和酶不稳定基团之间。 通过存在可以在单个菌落中检测到的荧光沉淀物来检测酶的存在，从而检测生物体。
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公开(公告)号：US06849236B2
公开(公告)日：2005-02-01
申请号：US09972160
申请日：2001-10-05
申请人： Urs Spitz , Albert Aichert , Willi Wälte
发明人： Urs Spitz , Albert Aichert , Willi Wälte
CPC分类号： G01N35/025 , G01N2035/0405 , Y10T436/11 , Y10T436/113332
摘要： An analyzer system has a base housing (1) and a sample tray (4) with at least two holding accommodations (5) for containers (32) of samples that are to be analyzed. The sample tray (4) is movably supported on the base housing. A drive mechanism (2) inside the base housing advances the tray (4) so that the containers (32) move in steps along a prescribed track to an analyzer module (16), where the samples are subjected to an analysis. A lid-opening device (50) is arranged along the prescribed track at a place that the sample containers (32) reach before they arrive at the analyzer module (16). The lid-opening device (50) serves to open lids (32′) of a predetermined shape and thickness (H) by which the sample containers (32) may be covered. The lids (32′) are preferably made of a non-magnetic material containing a magnet-anchor element.
摘要翻译： 分析仪系统具有底座外壳（1）和样品托盘（4），其具有用于要分析的样品的容器（32）的至少两个保持架（5）。 样品托盘（4）可移动地支撑在基座壳体上。 底座内部的驱动机构（2）使托盘（4）移动，使得容器（32）沿着规定的轨迹逐步移动到分析器模块（16），在分析器模块（16）处对样品进行分析。 在样品容器（32）到达分析器模块（16）之前到达的位置，沿着规定轨道布置开盖装置（50）。 盖打开装置（50）用于打开预定形状和厚度（H）的盖子（32'），通过该盖子可以覆盖样品容器（32）。 盖（32'）优选地由含有磁体 - 锚定元件的非磁性材料制成。
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