公开(公告)号：US11098337B2

公开(公告)日：2021-08-24

申请号：US14322827

申请日：2014-07-02

申请人： VIB VZW ,  UNIVERSITEIT GENT

发明人： Nico L. M. Callewaert ,  Karen De Pourcq ,  Steven Geysens ,  Leander Meuris

IPC分类号： C12N5/00 ,  C12N9/24 ,  C12P21/00 ,  A61K38/00

摘要： The disclosure relates to the field of glyco-engineering, more specifically, to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular, without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells are glyco-engineered yeast cells in which, additionally, at least one exogenous enzyme needed for complex glycosylation is present, e.g., allowing easier separation of differentially glycosylated glycoproteins.

公开(公告)号：US11293012B2

公开(公告)日：2022-04-05

申请号：US15741025

申请日：2016-07-08

申请人： VIB VZW ,  Universiteit Gent

发明人： Nico Callewaert ,  Leander Meuris ,  Francis Santens

IPC分类号： C12N9/24 ,  C12P21/00 ,  C12N9/90 ,  C07K14/505 ,  C07K14/535 ,  C07K14/005 ,  C07K14/715

摘要： The present application relates to the field of glyco-engineering and, more specifically, to eukaryotic cells wherein both an endoglucosaminidase is present and made deficient in UDP-galactose 4-epimerase (GalE). Typically, a glycoprotein is also present in the cells. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular, without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production.

公开(公告)号：US20180187177A1

公开(公告)日：2018-07-05

申请号：US15741025

申请日：2016-07-08

申请人： VIB VZW ,  Universiteit Gent

发明人： Nico Callewaert ,  Leander Meuris ,  Francis Santens

IPC分类号： C12N9/24 ,  C12N9/90 ,  C12P21/00 ,  C07K14/535 ,  C07K14/505 ,  C07K14/715 ,  C07K14/005

CPC分类号： C12N9/2402 ,  C07K14/005 ,  C07K14/505 ,  C07K14/535 ,  C07K14/7151 ,  C07K2319/02 ,  C07K2319/21 ,  C07K2319/30 ,  C12N9/90 ,  C12N2760/00051 ,  C12P21/005 ,  C12Y204/99001 ,  C12Y302/01096 ,  C12Y501/03002

摘要： The present application relates to the field of glyco-engineering and, more specifically, to eukaryotic cells wherein both an endoglucosaminidase is present and made deficient in UDP-galactose 4-epimerase (GalE). Typically, a glycoprotein is also present in the cells. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular, without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production.