公开(公告)号：US12129468B2

公开(公告)日：2024-10-29

申请号：US18427866

申请日：2024-01-31

Applicant: VedaBio, Inc.

Inventor： Anurup Ganguli ,  Ashish Pandey ,  Ariana Mostafa ,  Jacob Berger

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85

CPC classification number: C12N15/113 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/85 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods provide signal boost upon detection of target nucleic acids of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 16° C. or less, without amplification of the target nucleic acids yet allowing for massive multiplexing, high accuracy and minimal non-specific signal generation.

公开(公告)号：US11970730B2

公开(公告)日：2024-04-30

申请号：US18208272

申请日：2023-06-10

Applicant: VedaBio, Inc. ,  The Board of Trustees of the University of Illinois

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey ,  Rashid Bashir

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2320/10

Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising and a blocking nucleic acid molecule represented by Formula III in the 5′-to-3′ direction comprises: T-D-M-A-(B-L)J-C; wherein T is 17-135 nucleotides in length; D is 0-10 nucleotides in length; M is 1-25 nucleotides in length or is absent, wherein if M is absent then T-D and A-(B-L)J-C are separate nucleic acid strands; A is 0-15 nucleotides in length and comprises at least 50% sequence complementarity to D; B is 4-12 nucleotides in length and comprises at least 50% sequence complementarity to T; L is 3-25 nucleotides in length; J is an integer between 1 and 10; C is 4-15 nucleotides in length; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.

公开(公告)号：US11859182B2

公开(公告)日：2024-01-02

申请号：US18204329

申请日：2023-05-31

Applicant: VedaBio, Inc.

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey

IPC: C12N15/113 ,  C12N15/11

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/322

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.

公开(公告)号：US12247205B2

公开(公告)日：2025-03-11

申请号：US18805534

申请日：2024-08-15

Applicant: VedaBio, Inc.

Inventor： Ashish Pandey ,  Ariana Mostafa ,  Jacob Berger ,  Anurup Ganguli

IPC: C12N15/113 ,  C12N15/11

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.

公开(公告)号：US20240026425A1

公开(公告)日：2024-01-25

申请号：US18372098

申请日：2023-09-24

Applicant: VedaBio, Inc. ,  The Board of Trustees of the University of Illinois

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey ,  Rashid Bashir

IPC: C12Q1/6823 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682 ,  C12N9/22

CPC classification number: C12Q1/6823 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682 ,  C12N9/22 ,  C12N2320/10 ,  C12N2310/3231 ,  C12N2310/321 ,  C12N2310/315 ,  C12N2310/20

Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising and a blocking nucleic acid molecule represented by Formula IV, wherein Formula IV in the 5′-to-3′ direction comprises: T-D-M-A-Lp-C; wherein T is 17-31 nucleotides in length; D is 0-15 nucleotides in length; M is 1-25 nucleotides in length; A is 0-15 nucleotides in length and comprises a sequence complementary to D; and L is 3-25 nucleotides in length; p is 0 or 1; C is 4-15 nucleotides in length and comprises a sequence complementary to T; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.

公开(公告)号：US20240218428A1

公开(公告)日：2024-07-04

申请号：US18586486

申请日：2024-02-25

Applicant: VedaBio, Inc. ,  The Board of Trustees of the University of Illinois

Inventor： Ashish Pandey ,  Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2320/10

Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising a blocking nucleic acid molecule represented by Formula IV, wherein Formula IV in the 5′-to-3′ direction comprises: T-D-M-A-Lp-C; wherein T is 17-31 nucleotides in length; D is 0-15 nucleotides in length; M is 1-25 nucleotides in length; A is 0-15 nucleotides in length and comprises a sequence complementary to D; and L is 3-25 nucleotides in length; p is 0 or 1; C is 4-15 nucleotides in length and comprises a sequence complementary to T; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.

公开(公告)号：US11820983B2

公开(公告)日：2023-11-21

申请号：US18076262

申请日：2022-12-06

Applicant: VedaBio, Inc.

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey

IPC: C12N15/113 ,  C12N15/11

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/322

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.

公开(公告)号：US20240218364A1

公开(公告)日：2024-07-04

申请号：US18427866

申请日：2024-01-31

Applicant: VedaBio, Inc.

Inventor： Anurup Ganguli ,  Ashish Pandey ,  Ariana Mostafa ,  Jacob Berger

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85

CPC classification number: C12N15/113 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/85 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods provide signal boost upon detection of target nucleic acids of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 16° ° C. or less, without amplification of the target nucleic acids yet allowing for massive multiplexing, high accuracy and minimal non-specific signal generation.

公开(公告)号：US11987839B2

公开(公告)日：2024-05-21

申请号：US18372098

申请日：2023-09-24

Applicant: VedaBio, Inc. ,  The Board of Trustees of the University of Illinois

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2320/10

Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising a blocking nucleic acid molecule represented by Formula IV, wherein Formula IV in the 5′-to-3′ direction comprises: T-D-M-A-Lp-C; wherein T is 17-31 nucleotides in length; D is 0-15 nucleotides in length; M is 1-25 nucleotides in length; A is 0-15 nucleotides in length and comprises a sequence complementary to D; and L is 3-25 nucleotides in length; p is 0 or 1; C is 4-15 nucleotides in length and comprises a sequence complementary to T; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.

公开(公告)号：US11946052B1

公开(公告)日：2024-04-02

申请号：US18518914

申请日：2023-11-24

Applicant: VedaBio, Inc.

Inventor： Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey ,  Anurup Ganguli

IPC: C12N15/113 ,  C12N15/11

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/322

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.