公开(公告)号：US20250002977A1

公开(公告)日：2025-01-02

申请号：US18765750

申请日：2024-07-08

Applicant: VedaBio, Inc.

Inventor： Phillip Belgrader

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/64

Abstract: The present disclosure relates to multiplex assay methods used to detect target nucleic acids of interest from several to many sources in a sample without amplification of the target nucleic acids of interest. The methods involve partitioning of first ribonucleoprotein complexes to detect short sequences from several to many loci scattered throughout a single source genome, and the addition of signal boosting second ribonucleoprotein complexes and reporter moieties.

公开(公告)号：US12129468B2

公开(公告)日：2024-10-29

申请号：US18427866

申请日：2024-01-31

Applicant: VedaBio, Inc.

Inventor： Anurup Ganguli ,  Ashish Pandey ,  Ariana Mostafa ,  Jacob Berger

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85

CPC classification number: C12N15/113 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/85 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods provide signal boost upon detection of target nucleic acids of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 16° C. or less, without amplification of the target nucleic acids yet allowing for massive multiplexing, high accuracy and minimal non-specific signal generation.

公开(公告)号：US20240344044A1

公开(公告)日：2024-10-17

申请号：US18650024

申请日：2024-04-29

Applicant: VedaBio, Inc.

Inventor： Joshua Miller ,  Andrew Garst ,  Robert Plasschaert ,  Swetha Murali ,  Anurup Ganguli

IPC: C12N9/22

CPC classification number: C12N9/22 ,  C12N2310/122 ,  C12N2310/20

Abstract: The present disclosure relates to compositions of matter and methods used to activate effector nucleic acids and effector targets in vivo via a CRISPR-based cascade system. The compositions and methods achieve non-specific delivery of cascade system components to cells yet the cascade system works in a cell-specific manner.

公开(公告)号：US20240247304A1

公开(公告)日：2024-07-25

申请号：US18612868

申请日：2024-03-21

Applicant: VedaBio, Inc.

Inventor： Swetha Murali ,  Andrew Garst ,  Anurup Ganguli ,  Ketki Sawant ,  Ravi Vijayendran

IPC: C12Q1/682 ,  C12N9/22

CPC classification number: C12Q1/682 ,  C12N9/22 ,  C12N2310/20

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more non-nucleic acid targets of interest in a sample. The compositions and methods provide signal boost upon detection of non-nucleic acid targets of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 25° C. or less, allow for massive multiplexing, high accuracy, minimal non-specific signal generation, and are easily reprogrammable.

公开(公告)号：US11970730B2

公开(公告)日：2024-04-30

申请号：US18208272

申请日：2023-06-10

Applicant: VedaBio, Inc. ,  The Board of Trustees of the University of Illinois

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey ,  Rashid Bashir

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2320/10

Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising and a blocking nucleic acid molecule represented by Formula III in the 5′-to-3′ direction comprises: T-D-M-A-(B-L)J-C; wherein T is 17-135 nucleotides in length; D is 0-10 nucleotides in length; M is 1-25 nucleotides in length or is absent, wherein if M is absent then T-D and A-(B-L)J-C are separate nucleic acid strands; A is 0-15 nucleotides in length and comprises at least 50% sequence complementarity to D; B is 4-12 nucleotides in length and comprises at least 50% sequence complementarity to T; L is 3-25 nucleotides in length; J is an integer between 1 and 10; C is 4-15 nucleotides in length; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.

公开(公告)号：US11859182B2

公开(公告)日：2024-01-02

申请号：US18204329

申请日：2023-05-31

Applicant: VedaBio, Inc.

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey

IPC: C12N15/113 ,  C12N15/11

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/322

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods allow one to control reaction kinetics of the cascade assay by two orders of magnitude via molecular design of one of the reaction components; further, varying molecular design also allows for quantification of target nucleic acids of interest over a large range of concentrations or discriminating between extremely low copy numbers of target nucleic acids of interest.

公开(公告)号：US12091690B2

公开(公告)日：2024-09-17

申请号：US18402680

申请日：2024-01-02

Applicant: VedaBio, Inc.

Inventor： Andrew Garst ,  Anurup Ganguli ,  Ashish Pandey

IPC: C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6823

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6823 ,  C12N2310/20 ,  G01N2333/922

Abstract: The present disclosure relates to variant engineered nucleic acid-guided nucleases that may be used in CRISPR-based cascade assay systems to detect one or more target nucleic acids in a sample. The variant nucleases comprise an activity such that double-stranded DNA substrates do not bind to or are not cleaved by variant LbCas12a nuclease, or bind to or are cleaved very slowly by the variant nuclease, however single-stranded DNA substrates can bind and are cleaved by the variant nuclease, and wherein the variant nuclease exhibits both cis- and trans-cleavage activity.

公开(公告)号：US20240218364A1

公开(公告)日：2024-07-04

申请号：US18427866

申请日：2024-01-31

Applicant: VedaBio, Inc.

Inventor： Anurup Ganguli ,  Ashish Pandey ,  Ariana Mostafa ,  Jacob Berger

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85

CPC classification number: C12N15/113 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/85 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods provide signal boost upon detection of target nucleic acids of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 16° ° C. or less, without amplification of the target nucleic acids yet allowing for massive multiplexing, high accuracy and minimal non-specific signal generation.

公开(公告)号：US11987839B2

公开(公告)日：2024-05-21

申请号：US18372098

申请日：2023-09-24

Applicant: VedaBio, Inc. ,  The Board of Trustees of the University of Illinois

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2320/10

Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising a blocking nucleic acid molecule represented by Formula IV, wherein Formula IV in the 5′-to-3′ direction comprises: T-D-M-A-Lp-C; wherein T is 17-31 nucleotides in length; D is 0-15 nucleotides in length; M is 1-25 nucleotides in length; A is 0-15 nucleotides in length and comprises a sequence complementary to D; and L is 3-25 nucleotides in length; p is 0 or 1; C is 4-15 nucleotides in length and comprises a sequence complementary to T; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.

公开(公告)号：US11965205B1

公开(公告)日：2024-04-23

申请号：US18377294

申请日：2023-10-05

Applicant: VedaBio, Inc.

Inventor： Swetha Murali ,  Anurup Ganguli ,  Ketki Sawant ,  Ravi Vijayendran

IPC: C12Q1/682 ,  C12N9/22

CPC classification number: C12Q1/682 ,  C12N9/22 ,  C12N2310/20

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more non-nucleic acid targets of interest in a sample. The compositions and methods provide signal boost upon detection of non-nucleic acid targets of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 25° C. or less, allow for massive multiplexing, high accuracy, minimal non-specific signal generation, and are easily reprogrammable.