公开(公告)号：US20250002977A1

公开(公告)日：2025-01-02

申请号：US18765750

申请日：2024-07-08

Applicant: VedaBio, Inc.

Inventor： Phillip Belgrader

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/64

Abstract: The present disclosure relates to multiplex assay methods used to detect target nucleic acids of interest from several to many sources in a sample without amplification of the target nucleic acids of interest. The methods involve partitioning of first ribonucleoprotein complexes to detect short sequences from several to many loci scattered throughout a single source genome, and the addition of signal boosting second ribonucleoprotein complexes and reporter moieties.

公开(公告)号：US12258619B2

公开(公告)日：2025-03-25

申请号：US18765750

申请日：2024-07-08

Applicant: VedaBio, Inc.

Inventor： Phillip Belgrader

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/64

Abstract: The present disclosure relates to multiplex assay methods used to detect target nucleic acids of interest from several to many sources in a sample without amplification of the target nucleic acids of interest. The methods involve partitioning of first ribonucleoprotein complexes to detect short sequences from several to many loci scattered throughout a single source genome, and the addition of signal boosting second ribonucleoprotein complexes and reporter moieties.

公开(公告)号：US20240287623A1

公开(公告)日：2024-08-29

申请号：US18586420

申请日：2024-02-23

Applicant: VedaBio, Inc.

Inventor： Don Masquelier ,  Phillip Belgrader ,  Anurup Ganguli

IPC: C12Q1/689 ,  C12Q1/6823 ,  C12Q1/6827

CPC classification number: C12Q1/689 ,  C12Q1/6823 ,  C12Q1/6827 ,  C12Q2600/156

Abstract: The present disclosure relates to multiplex assay methods and systems used to detect several to many to a massively multiplexed number of target nucleic acids of interest in a sample without amplification of the target nucleic acids of interest. The method employs microfluidic droplet systems where each droplet is a “mini-reactor.” In some embodiments, a “bulk format” configuration is used, in other embodiments, a “sequential format” configuration is used.

公开(公告)号：US12060602B2

公开(公告)日：2024-08-13

申请号：US18406159

申请日：2024-01-07

Applicant: VedaBio, Inc.

Inventor： Phillip Belgrader

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/64

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/111 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/6486 ,  C12N2310/20

Abstract: The present disclosure relates to multiplex assay methods used to detect target nucleic acids of interest from several to many sources in a sample without amplification of the target nucleic acids of interest. The methods involve partitioning of first ribonucleoprotein complexes to detect short sequences from several to many loci scattered throughout a single source genome, and the addition of signal boosting second ribonucleoprotein complexes and reporter moieties.

公开(公告)号：US20240240238A1

公开(公告)日：2024-07-18

申请号：US18406159

申请日：2024-01-07

Applicant: VedaBio, Inc.

Inventor： Phillip Belgrader

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/64

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/111 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/6486 ,  C12N2310/20

Abstract: The present disclosure relates to multiplex assay methods used to detect target nucleic acids of interest from several to many sources in a sample without amplification of the target nucleic acids of interest. The methods involve partitioning of first ribonucleoprotein complexes to detect short sequences from several to many loci scattered throughout a single source genome, and the addition of signal boosting second ribonucleoprotein complexes and reporter moieties.