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    TEMPERATURE-REGULATED CULTURE PLATES
    1.
    发明申请
    TEMPERATURE-REGULATED CULTURE PLATES 审中-公开
    温度调节文化板

    公开(公告)号：US20100009335A1

    公开(公告)日：2010-01-14

    申请号：US12278570

    申请日：2007-02-07

    申请人： Victor Joseph ,  Amjad Huda ,  Joseph F. Rogers

    发明人： Victor Joseph ,  Amjad Huda ,  Joseph F. Rogers

    IPC分类号： C12Q3/00 ,  C12M1/36

    CPC分类号： C12M23/48 ,  C12M23/12 ,  C12M23/22 ,  C12M41/12 ,  C12M41/26 ,  C12M41/32 ,  C12M41/44

    摘要： Described herein are environmentally isolated tissue culture devices that may be used for cell culture, as well as systems including these devices and methods for using them. These devices may include control features for regulating the micro-environment within a well or wells of the device. For example, on-board features may regulate the temperature, humidity, pH, media level, media composition, CO2/O2/N2 levels, drug concentration, cell density, byproduct (or product) production, and mixing of materials within the chamber. Material may be added to or withdrawn from the wells of the device without opening the device. Also described herein are controllers for analyzing and controlling the micro- environment within the well. Thus, the plates described herein may be used without requiring a separate incubator, allowing cells to be analyzed (e.g., imaged) continuously, allowing real-time reactions while monitoring under a microscope for hours, days or even weeks.

    摘要翻译： 本文描述了可用于细胞培养的环境分离的组织培养装置，以及包括这些装置的系统和使用它们的方法。 这些装置可以包括用于调节装置的井或井内的微环境的控制特征。 例如，车载功能可以调节室内的温度，湿度，pH，介质水平，介质组成，CO2 / O2 / N2水平，药物浓度，细胞密度，副产物（或产品）生产和混合物质。 可以在不打开设备的情况下将材料添加到设备的井中或从设备的井中取出。 本文还描述了用于分析和控制井内微环境的控制器。 因此，可以使用本文所述的板，而不需要单独的培养箱，允许细胞连续分析（例如，成像），允许实时反应，同时在显微镜下监测数小时，数天甚至数周。