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公开(公告)号：US20050164210A1
公开(公告)日：2005-07-28
申请号：US10763479
申请日：2004-01-23
申请人： Vivek Mittal , Sunita Gupta , Gregory Hannon , Patrick Paddison , Eric Julien , Winship Herr
发明人： Vivek Mittal , Sunita Gupta , Gregory Hannon , Patrick Paddison , Eric Julien , Winship Herr
CPC分类号： C12N15/1135 , A01K2217/05 , C07H21/04 , C12N15/111 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30 , C12N2799/027 , C12Q1/6897
摘要： The invention provides, among other things, regulated polymerase III expression systems and related compositions. The invention provides, in part, expression systems in which the expression is inducible, and systems which express inhibitory RNA molecules, such as hairpin RNA molecules. The invention also provides related methods, such as methods of inhibiting expression of a gene.
摘要翻译： 本发明尤其提供了调节的聚合酶III表达系统和相关组合物。 本发明部分提供其中表达是可诱导的表达系统，以及表达抑制性RNA分子的系统，例如发夹RNA分子。 本发明还提供了相关方法，例如抑制基因表达的方法。
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公开(公告)号：US20060135456A1
公开(公告)日：2006-06-22
申请号：US10997086
申请日：2004-11-23
申请人： Gregory Hannon , Patrick Paddison , Despina Siolas
发明人： Gregory Hannon , Patrick Paddison , Despina Siolas
IPC分类号： A61K48/00
CPC分类号： C12N15/113 , A01K2217/05 , A61K38/00 , C12N15/1034 , C12N15/1079 , C12N15/111 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/12 , C12N2330/30 , C12N2330/31 , C12Y301/26003
摘要： The present invention provides methods for attenuating gene expression in a cell, especially in a mammalian cell, using gene-targeted double stranded RNA (dsRNA), such as a hairpin RNA. The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the “target” gene).
摘要翻译： 本发明提供使用基因靶向双链RNA（dsRNA）如发夹RNA来减弱细胞，特别是哺乳动物细胞中的基因表达的方法。 dsRNA含有在细胞的生理条件下与要抑制的基因的至少一部分（“靶”基因）的核苷酸序列杂交的核苷酸序列。
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公开(公告)号：US20060195918A1
公开(公告)日：2006-08-31
申请号：US11332943
申请日：2006-01-17
申请人： Ihor Lemischka , Christoph Schaniel , Feng Li , Xenia Schafer , Patrick Paddison
发明人： Ihor Lemischka , Christoph Schaniel , Feng Li , Xenia Schafer , Patrick Paddison
IPC分类号： A01K67/027 , C12Q1/00 , C12N5/06 , C12N5/08
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/105 , A01K2267/03 , G01N33/5023 , G01N33/5073
摘要： The present invention relates to methods and compositions for assaying embryonic stem cell maintenance. In particular, the present invention provides reporter constructs for stem cell pluripotency and differentiation and cells and organisms containing such constructs.
摘要翻译： 本发明涉及用于测定胚胎干细胞维持的方法和组合物。 特别地，本发明提供用于干细胞多能性和分化的报道构建体以及含有这种构建体的细胞和生物体。
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公开(公告)号：US08829264B2
公开(公告)日：2014-09-09
申请号：US13526335
申请日：2012-06-18
申请人： Gregory J. Hannon , Patrick Paddison , Emily Bernstein , Amy Caudy , Douglas Conklin , Scott Hammond
发明人： Gregory J. Hannon , Patrick Paddison , Emily Bernstein , Amy Caudy , Douglas Conklin , Scott Hammond
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A01K2217/05 , A61K38/00 , C12N15/1034 , C12N15/1079 , C12N15/111 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/12 , C12N2330/30 , C12N2330/31 , C12Y301/26003
摘要： The present invention provides methods for attenuating gene expression in a cell, especially in a mammalian cell, using gene-targeted double stranded RNA (dsRNA), such as a hairpin RNA. The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the “target” gene).
摘要翻译： 本发明提供使用基因靶向双链RNA（dsRNA）如发夹RNA来减弱细胞，特别是哺乳动物细胞中的基因表达的方法。 dsRNA含有在细胞的生理条件下与要抑制的基因的至少一部分（“靶”基因）的核苷酸序列杂交的核苷酸序列。
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公开(公告)号：US08753811B2
公开(公告)日：2014-06-17
申请号：US13398560
申请日：2012-02-16
申请人： Stephen H. Friend , Michele A. Cleary , Ernest M. Coffey , Kristopher A. Killian , Gregory J. Hannon , Patrick Paddison
发明人： Stephen H. Friend , Michele A. Cleary , Ernest M. Coffey , Kristopher A. Killian , Gregory J. Hannon , Patrick Paddison
CPC分类号： C12Q1/6837 , Y10S977/792 , C12Q2565/513 , C12Q2531/113 , C12Q2525/161
摘要： The present invention provides methods of making a population of nucleic acid molecules, wherein each nucleic acid molecule comprises a predetermined nucleic acid sequence, each of said methods comprising the steps of: (a) synthesizing, on a substrate, a population of nucleic acid molecules wherein: i) each synthesized nucleic acid molecule comprises a predetermined nucleic acid sequence; and ii) each synthesized nucleic acid molecule is localized to a defined area of said substrate; (b) harvesting said population of synthesized nucleic acid molecules from said substrate to yield harvested nucleic acid molecules; and (c) introducing said harvested nucleic acid molecules into vector molecules.
摘要翻译： 本发明提供了制备核酸分子群体的方法，其中每个核酸分子包含预定的核酸序列，所述方法中的每一种包括以下步骤：（a）在底物上合成核酸分子群体 其中：i）每个合成的核酸分子包含预定的核酸序列; 和ii）每个合成的核酸分子定位于所述基质的限定区域; （b）从所述底物收获所述合成核酸分子群体以产生收获的核酸分子; 和（c）将所述收获的核酸分子引入载体分子。
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公开(公告)号：US08133670B2
公开(公告)日：2012-03-13
申请号：US10860559
申请日：2004-06-02
申请人： Stephen H Friend , Michele A Cleary , Kristopher A Kilian , Ernest M Coffey , Gregory J Hannon , Patrick Paddison
发明人： Stephen H Friend , Michele A Cleary , Kristopher A Kilian , Ernest M Coffey , Gregory J Hannon , Patrick Paddison
CPC分类号： C12Q1/6837 , Y10S977/792 , C12Q2565/513 , C12Q2531/113 , C12Q2525/161
摘要： The present invention provides methods of making a population of nucleic acid molecules, wherein each nucleic acid molecule comprises a predetermined nucleic acid sequence, each of said methods comprising the steps of: (a) synthesizing, on a substrate, a population of nucleic acid molecules wherein: i) each synthesized nucleic acid molecule comprises a predetermined nucleic acid sequence; and ii) each synthesized nucleic acid molecule is localized to a defined area of said substrate; (b) harvesting said population of synthesized nucleic acid molecules from said substrate to yield harvested nucleic acid molecules; and (c) introducing said harvested nucleic acid molecules into vector molecules.
摘要翻译： 本发明提供了制备核酸分子群体的方法，其中每个核酸分子包含预定的核酸序列，所述方法中的每一种包括以下步骤：（a）在底物上合成核酸分子群体 其中：i）每个合成的核酸分子包含预定的核酸序列; 和ii）每个合成的核酸分子定位于所述基质的限定区域; （b）从所述底物收获所述合成核酸分子群体以产生收获的核酸分子; 和（c）将所述收获的核酸分子引入载体分子。
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公开(公告)号：US20130276158A1
公开(公告)日：2013-10-17
申请号：US13526335
申请日：2012-06-18
申请人： Gregory J. HANNON , Patrick Paddison , Emily Bernstein , Amy Caudy , Douglas Conklin , Scott Hammond
发明人： Gregory J. HANNON , Patrick Paddison , Emily Bernstein , Amy Caudy , Douglas Conklin , Scott Hammond
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A01K2217/05 , A61K38/00 , C12N15/1034 , C12N15/1079 , C12N15/111 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/12 , C12N2330/30 , C12N2330/31 , C12Y301/26003
摘要： The present invention provides methods for attenuating gene expression in a cell, especially in a mammalian cell, using gene-targeted double stranded RNA (dsRNA), such as a hairpin RNA. The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the “target” gene).
摘要翻译： 本发明提供使用基因靶向双链RNA（dsRNA）如发夹RNA来减弱细胞，特别是哺乳动物细胞中的基因表达的方法。 dsRNA含有在细胞的生理条件下与要抑制的基因的至少一部分（“靶”基因）的核苷酸序列杂交的核苷酸序列。
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公开(公告)号：US20050059077A1
公开(公告)日：2005-03-17
申请号：US10973461
申请日：2004-10-27
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6886 , C12Q1/6895 , C12Q2600/136
摘要： The invention relates to novel nucleotide sequences and the polypeptide sequences derived therefrom, which are involved in yeast and mammalian telomere regulation. Methods for screening drugs and compositions are also provided. The invention furthermore relates to methods involving a diagnostic and/or therapeutic use of these gene(s)/protein(s), principally for treating cancer and aging.
摘要翻译： 本发明涉及新型核苷酸序列及其衍生的多肽序列，其涉及酵母和哺乳动物端粒调节。 还提供了筛选药物和组合物的方法。 本发明还涉及涉及这些基因/蛋白质的诊断和/或治疗用途的方法，主要用于治疗癌症和衰老。
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公开(公告)号：US08202846B2
公开(公告)日：2012-06-19
申请号：US10997086
申请日：2004-11-23
申请人： Gregory J. Hannon , Patrick Paddison , Emily Bernstein , Amy Caudy , Douglas Conklin , Scott Hammond
发明人： Gregory J. Hannon , Patrick Paddison , Emily Bernstein , Amy Caudy , Douglas Conklin , Scott Hammond
IPC分类号： A61K48/00
CPC分类号： C12N15/113 , A01K2217/05 , A61K38/00 , C12N15/1034 , C12N15/1079 , C12N15/111 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/12 , C12N2330/30 , C12N2330/31 , C12Y301/26003
摘要： The present invention provides methods for attenuating gene expression in a cell, especially in a mammalian cell, using gene-targeted double stranded RNA (dsRNA), such as a hairpin RNA. The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the “target” gene).
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公开(公告)号：US08153776B2
公开(公告)日：2012-04-10
申请号：US11894676
申请日：2007-08-20
申请人： Gregory J. Hannon , Patrick Paddison , Emily Bernstein , Amy Caudy , Douglas Conklin , Scott Hammond
发明人： Gregory J. Hannon , Patrick Paddison , Emily Bernstein , Amy Caudy , Douglas Conklin , Scott Hammond
IPC分类号： C07H21/04
CPC分类号： C12N15/113 , A01K2217/05 , A61K38/00 , C12N15/1034 , C12N15/1079 , C12N15/111 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/12 , C12N2330/30 , C12N2330/31 , C12Y301/26003
摘要： The present invention provides methods for attenuating gene expression in a cell, especially in a mammalian cell, using gene-targeted double stranded RNA (dsRNA), such as a hairpin RNA. The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the “target” gene).
摘要翻译： 本发明提供使用基因靶向双链RNA（dsRNA）如发夹RNA来减弱细胞，特别是哺乳动物细胞中的基因表达的方法。 dsRNA含有在细胞的生理条件下与要抑制的基因的至少一部分（“靶”基因）的核苷酸序列杂交的核苷酸序列。
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