公开(公告)号：US20110212105A1

公开(公告)日：2011-09-01

申请号：US12298808

申请日：2007-04-26

申请人： Vivian Huerta Galindo ,  Glay Chinea Santiago ,  Noralvis Fleitas Salazar ,  Alejandro Miguel Martín Dunn ,  Mónica Sarría Núñez ,  Osmany Guirola Cruz ,  Patricia Gabriela Toledo Mayora ,  Aniel Sánchez Puente ,  Vladimir Armando Besada Pérez ,  Osvaldo Reyes Acosta ,  Hilda Elisa Garay Pérez ,  Ania Cabrales Rico ,  Alexis Musacchio Lasa ,  Gabriel Ramón Padrón Palomares ,  Luis Javier González López

发明人： Vivian Huerta Galindo ,  Glay Chinea Santiago ,  Noralvis Fleitas Salazar ,  Alejandro Miguel Martín Dunn ,  Mónica Sarría Núñez ,  Osmany Guirola Cruz ,  Patricia Gabriela Toledo Mayora ,  Aniel Sánchez Puente ,  Vladimir Armando Besada Pérez ,  Osvaldo Reyes Acosta ,  Hilda Elisa Garay Pérez ,  Ania Cabrales Rico ,  Alexis Musacchio Lasa ,  Gabriel Ramón Padrón Palomares ,  Luis Javier González López

IPC分类号： A61K39/42 ,  A61K38/16 ,  C07K7/08

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K14/70596 ,  C07K14/8107 ,  C12N2770/24122 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394 ,  Y02A50/396

摘要： The present invention is related to a method for blocking the infection of cells by dengue virus, based on interfering the direct interaction of the viral envelope protein with a cellular receptor or its indirect interaction with said cellular receptor through a carrier protein, as well as related uses; wherein said cellular receptor is the alpha-2 macroglobulin receptor, also known as the low density receptor-related protein or as CD91, and said carrier protein is human alpha-2 macroglobulin.

摘要翻译： 本发明涉及一种基于干扰病毒包膜蛋白与细胞受体的直接相互作用或其通过载体蛋白与所述细胞受体的间接相互作用而阻断登革热病毒感染细胞的方法，以及相关的 用途; 其中所述细胞受体是α-2巨球蛋白受体，也称为低密度受体相关蛋白或CD91，所述载体蛋白是人α-2巨球蛋白。

公开(公告)号：US07244411B2

公开(公告)日：2007-07-17

申请号：US10988943

申请日：2004-11-15

申请人： Lázaro Hiram Betancourt Núñez ,  Jorge Fernández de Cossio Dorta-Duque ,  Vladimir Armando Besada Pérez ,  Jeovanis Gil Valdés ,  Luis Javier González López ,  Gabriel Ramón Padrón Palomares ,  Rolando Pajón Feyt ,  Félix Modesto Álvarez Gil

发明人： Lázaro Hiram Betancourt Núñez ,  Jorge Fernández de Cossio Dorta-Duque ,  Vladimir Armando Besada Pérez ,  Jeovanis Gil Valdés ,  Luis Javier González López ,  Gabriel Ramón Padrón Palomares ,  Rolando Pajón Feyt ,  Félix Modesto Álvarez Gil

IPC分类号： A61K51/00 ,  A61M36/14

CPC分类号： G01N33/6848 ,  C12Q1/37

摘要： The present invention describes a method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixture. The method comprises the selective isolation from every protein of those peptides that neither contain arginine nor histidine (NHNR peptides), and the determination of the relative concentrations of one or several proteins in different samples from the ratio between the areas of the estimated theoretical spectra for the NHNR peptides labeled with different isotopes in each sample. The determination of the relative concentration of proteins is valid for any type of isotopic label of the NHNR peptides. The method avoids the separation and purification of the proteins present in a complex mixture, and the analysis of all peptides generated from the enzymatic digest of the samples. The method is applicable to the identification of proteins with vacunal, therapeutic and diagnostic aims.

摘要翻译： 本发明描述了用于鉴定和定量分析复合物中蛋白质的选择性肽分离方法。 该方法包括从不含精氨酸和组氨酸（NHNR肽）的那些肽的每种蛋白质的选择性分离，以及将不同样品中一种或几种蛋白质的相对浓度与估计的理论光谱面积之比 在每个样品中用不同同位素标记的NHNR肽。 蛋白质的相对浓度的测定对于NHNR肽的任何类型的同位素标记是有效的。 该方法避免了复杂混合物中存在的蛋白质的分离和纯化，以及从样品的酶促消化产生的所有肽的分析。 该方法适用于鉴定具有病毒，治疗和诊断目的的蛋白质。