公开(公告)号：US20210349060A1

公开(公告)日：2021-11-11

申请号：US17307389

申请日：2021-05-04

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Sylvain Gilles Cormier ,  Thomas Swann ,  Francis Patrick Tracey

IPC: G01N30/46

Abstract: Described are multidimensional liquid chromatography (MDLC) systems and methods of performing MDLC. The MDLC systems includes valve configurations that enable a flow containing analytes in the eluent of a first dimension liquid chromatography system to be modulated such that the analytes can be captured in fluidic loops and subsequently provided to a second dimension liquid chromatography system. Various system embodiments are provided in a smaller instrument size, include an option to use the same detector for both first and second dimensions, enable improvement in valve timing operations and provide a reduction in the magnitude and effects of pressure pulses. In addition, the modulator can enable dilution of the analytes and enable incompatible mobile phases and mobile phase flow rates to be used in the first and second dimensions.

公开(公告)号：US20230204478A1

公开(公告)日：2023-06-29

申请号：US18166738

申请日：2023-02-09

Applicant: Waters Technologies Corporation

Inventor： Bonnie A. Alden ,  Kenneth Berthelette ,  Jon Finch ,  Donna Osterman ,  Thomas Swann ,  Thomas H. Walter

IPC: G01N1/40 ,  B01D15/42 ,  B01D15/36 ,  B01J39/26 ,  B01J39/05 ,  B01D15/32 ,  B01D15/38 ,  G01N33/92

CPC classification number: G01N1/405 ,  B01D15/426 ,  B01D15/362 ,  B01J39/26 ,  B01J39/05 ,  B01D15/327 ,  B01D15/3847 ,  G01N33/92 ,  G01N2030/062

Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.

公开(公告)号：US20230366858A1

公开(公告)日：2023-11-16

申请号：US18358224

申请日：2023-07-25

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Sylvain Gilles Cormier ,  Thomas Swann ,  Francis Patrick Tracey

IPC: G01N30/46 ,  G01N30/02

CPC classification number: G01N30/468 ,  G01N30/463 ,  G01N2030/027

Abstract: Multidimensional liquid chromatography (MDLC) systems include valve configurations that enable a flow containing analytes in the eluent of a first dimension liquid chromatography system to be modulated such that the analytes can be captured in fluidic loops and subsequently provided to a second dimension liquid chromatography system. Optionally, a single detector is used for both first and second dimensions. In addition, the systems can enable dilution of the analytes and enable incompatible mobile phases and mobile phase flow rates to be used in the first and second dimensions.

公开(公告)号：US20190195758A1

公开(公告)日：2019-06-27

申请号：US16230567

申请日：2018-12-21

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Bonnie A. Alden ,  Kenneth Berthelette ,  Jon Finch ,  Donna Osterman ,  Thomas Swann ,  Thomas H. Walter

IPC: G01N1/40 ,  B01J39/26 ,  B01D15/36 ,  B01D15/42

CPC classification number: G01N1/405 ,  B01D15/327 ,  B01D15/362 ,  B01D15/3847 ,  B01D15/426 ,  B01J39/05 ,  B01J39/26 ,  G01N2030/009 ,  G01N2030/062

Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.

公开(公告)号：US11747312B2

公开(公告)日：2023-09-05

申请号：US17307389

申请日：2021-05-04

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Sylvain Gilles Cormier ,  Thomas Swann ,  Francis Patrick Tracey

IPC: G01N30/46 ,  G01N30/02

CPC classification number: G01N30/468 ,  G01N30/463 ,  G01N2030/027

Abstract: Multidimensional liquid chromatography (MDLC) systems include valve configurations that enable a flow containing analytes in the eluent of a first dimension liquid chromatography system to be modulated such that the analytes can be captured in fluidic loops and subsequently provided to a second dimension liquid chromatography system. Optionally, a single detector is used for both first and second dimensions. In addition, the systems can enable dilution of the analytes and enable incompatible mobile phases and mobile phase flow rates to be used in the first and second dimensions.

公开(公告)号：US11604123B2

公开(公告)日：2023-03-14

申请号：US16230567

申请日：2018-12-21

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Bonnie A. Alden ,  Kenneth Berthelette ,  Jon Finch ,  Donna Osterman ,  Thomas Swann ,  Thomas H. Walter

IPC: G01N1/40 ,  B01D15/38 ,  B01D15/42 ,  B01D15/36 ,  B01J39/26 ,  B01J39/05 ,  B01D15/32 ,  G01N33/92 ,  G01N30/06 ,  G01N30/00

Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.