公开(公告)号：US20210162017A1

公开(公告)日：2021-06-03

申请号：US17105156

申请日：2020-11-25

Applicant: Wake Forest University Health Sciences

Inventor： James YOO ,  Sang Jin Lee ,  Anthony Atala ,  Mark Van Dyke

IPC: A61K38/39 ,  A61K9/00 ,  A61K38/48 ,  A61L27/24 ,  A61L27/52 ,  A61L27/54

Abstract: The invention is directed to methods of inducing cell recruitment and tissue regeneration at a target site in a subject. It is also based, in part, on the discovery that a subject's own biologic resources and environmental conditions can be used for in situ tissue regeneration and thereby reduce or eliminate the need for donor cell procurement and ex vivo manipulation of such donor cells. Methods are disclosed for recruitment of a subject's own stem cells to a target region by inducing a sustained positive pressure at a target site, such as the kidney, thereby increasing the number of pluripotent cells capable of differentiating to regenerate the target tissue.

公开(公告)号：US20220378844A1

公开(公告)日：2022-12-01

申请号：US17770971

申请日：2020-10-26

Applicant: Wake Forest University Health Sciences

Inventor： Anthony ATALA ,  James YOO ,  Gary G. POEHLING ,  Brian Robert WATERMAN

IPC: A61K35/34 ,  C12N5/077 ,  A61L27/36 ,  A61L27/38 ,  A61K38/18

Abstract: Methods and compositions are disclosed for repair of shoulder injuries by employing disaggregated muscle fiber fragments to regenerate functional shoulder muscle tissue. In some embodiments, the fragments retain functional satellite cells but exhibit cell wall rupture and have an average size of less than 150 μm. The methods include the preparation and implantation of compositions by extracting muscle tissue from a donor site, disaggregating muscle fibers from the extracted tissue, and fragmenting disaggregated muscle fibers into fiber fragments that exhibit cell wall rupture and preferably have an average size of less than 150 microns, more preferable less than about 100 microns, while retaining functional satellite cells. Upon injection, e.g., into the supraspinatus or other rotator cuff muscles, the muscle fiber fragment compositions are capable of reconstituting or reconstructing elongated muscle fibers from the fragments and orienting in alignment with native shoulder muscle fibers.

公开(公告)号：US20180214614A1

公开(公告)日：2018-08-02

申请号：US15747352

申请日：2016-07-21

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： James YOO ,  In Kap KO ,  Jennifer HULING ,  Anthony ATALA

IPC: A61L27/56 ,  A61L27/38 ,  A61L27/52 ,  A61L27/50

CPC classification number: A61L27/56 ,  A61L27/20 ,  A61L27/24 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3821 ,  A61L27/3826 ,  A61L27/50 ,  A61L27/507 ,  A61L27/52 ,  A61L2430/20 ,  A61L2430/22 ,  A61L2430/28 ,  A61L2430/30

Abstract: The present application relates to biomimetic three-dimensional (3D) scaffolds, constructs and methods of making the same. The three-dimensional scaffold can include a sacrificial internal cast and a durable external scaffold material, wherein the durable external scaffold material comprises a biocompatible material which completely surrounds the sacrificial internal cast and wherein the sacrificial internal cast be removed to yield a branching 3D network of hollow, vessel-like tubes that substantially mimics a native tissue or organ.