公开(公告)号：US11318106B2

公开(公告)日：2022-05-03

申请号：US16690558

申请日：2019-11-21

Applicant: Wake Forest University Health Sciences

Inventor： Benjamin S. Harrison ,  James J. Yoo ,  Anthony Atala

IPC: A61L15/26 ,  A61K9/70 ,  A61K31/77 ,  A61L15/18 ,  A61L15/64 ,  A61L26/00 ,  A61L27/44 ,  A61L27/58 ,  A61K38/00

Abstract: A method of treating hypoxic tissue such as wound tissue comprises contacting a composition to the hypoxic tissue in a hypoxia-treatment effective amount, the composition comprising a biodegradable polymer and an inorganic peroxide incorporated into the polymer.

公开(公告)号：US11230702B2

公开(公告)日：2022-01-25

申请号：US16322232

申请日：2017-08-04

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Robert T. Wicks ,  Anthony Atala ,  Goodwell Nzou ,  Elizabeth E. Wicks

IPC: C12N5/071 ,  C12N5/00

Abstract: Provided herein is an in vitro model of the blood brain barrier. In some embodiments, the model includes: an endothelial cell layer, and brain tissue layer comprising neuronal cells, and optionally one or more of astrocytes, pericytes, oligodendrocytes, and microglia. In some embodiments, the model further comprises a porous membrane between said endothelial cell layer and the neuronal cell layer. A microfluidic device comprising the same and methods of use thereof are also provided.

公开(公告)号：US20210162017A1

公开(公告)日：2021-06-03

申请号：US17105156

申请日：2020-11-25

Applicant: Wake Forest University Health Sciences

Inventor： James YOO ,  Sang Jin Lee ,  Anthony Atala ,  Mark Van Dyke

IPC: A61K38/39 ,  A61K9/00 ,  A61K38/48 ,  A61L27/24 ,  A61L27/52 ,  A61L27/54

Abstract: The invention is directed to methods of inducing cell recruitment and tissue regeneration at a target site in a subject. It is also based, in part, on the discovery that a subject's own biologic resources and environmental conditions can be used for in situ tissue regeneration and thereby reduce or eliminate the need for donor cell procurement and ex vivo manipulation of such donor cells. Methods are disclosed for recruitment of a subject's own stem cells to a target region by inducing a sustained positive pressure at a target site, such as the kidney, thereby increasing the number of pluripotent cells capable of differentiating to regenerate the target tissue.

公开(公告)号：US11013825B2

公开(公告)日：2021-05-25

申请号：US15672885

申请日：2017-08-09

Applicant: Wake Forest University Health Sciences

Inventor： Hyun-Wook Kang ,  Sang Jin Lee ,  Anthony Atala ,  James J. Yoo

IPC: B29C64/112 ,  A61L27/38 ,  A61L27/20 ,  B29C64/393 ,  A61L27/22 ,  A61L27/52 ,  B29C64/124 ,  A61L27/26 ,  C12M3/00 ,  C12M1/00 ,  C12M1/26 ,  A61K35/12 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  B33Y80/00 ,  B29K105/00 ,  B29K267/00 ,  B29L31/00

Abstract: A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.

公开(公告)号：US20210047375A1

公开(公告)日：2021-02-18

申请号：US17051296

申请日：2019-05-01

Applicant: Wake Forest University Health Sciences

Inventor： Baisong Lu ,  Anthony Atala

IPC: C07K14/155 ,  C12N9/22 ,  C12N15/86 ,  C12N15/113

Abstract: Provided are compositions, systems, and methods useful for effecting gene editing in eukaryotic cells. Compositions include plasmids that encode one or more viral fusion proteins in which one or more viral proteins are fused with an aptamer-binding protein. Compositions also include plasmids that encode a non-viral nucleic acid sequence, wherein the non-viral nucleic acid sequence encodes a CRISPR system component. In some instances, the non-viral nucleic acid sequence also includes an aptamer sequence. The plasmids can be used to generate viral particles, including lentivirus-like particles that contain a viral fusion protein and a non-viral RNA sequence. Systems of producing such viral particles are provided. Also provided are methods of using the viral particles of the disclosure to effect gene editing in eukaryotic cells.

公开(公告)号：US10751448B2

公开(公告)日：2020-08-25

申请号：US15542095

申请日：2016-01-11

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  Gayoung Jeong ,  James J. Yoo ,  Sang jin Lee ,  Young-Joon Seol

IPC: A61L27/60 ,  A61F2/10 ,  B33Y80/00 ,  B33Y70/00 ,  C08L5/08 ,  A61L27/20 ,  C08L89/06 ,  C12N5/071 ,  B33Y10/00 ,  B29C64/106 ,  A61L27/18 ,  A61L27/38 ,  A61L27/52 ,  G01N33/50 ,  B29K75/00 ,  B29K105/00

Abstract: Provided are live, artificial, skin substitute products and methods of making and using the same, such as for wound treatment and compound testing, including compound testing for efficacy, toxicity, penetration, irritation and/or metabolism testing of drug candidates or compositions such as cosmetics. Described herein is an artificial mammalian skin substitute product, comprising: (a) optionally, but in some embodiments preferably, a first (“hypodermis-like”) layer comprising live mammalian adipocytes (e.g., induced pre-adipocytes) in a first hydrogel carrier; (b) a second (“dermis-like”) layer contacting or directly contacting the first layer and comprising live mammalian fibroblast cells and' live mammalian follicle dermal papilla cells in combination in a second hydrogel carrier; (c) a third (“epidermis-like”) layer contacting or directly contacting the second layer (i.e., on the opposite side thereof as the first layer, so that the second layer is sandwiched between the first and third layers when the first layer is present), the third layer comprising live mammalian keratinocytes and live mammalian melanocytes in combination in a third hydrogel carrier.

公开(公告)号：US20200085579A1

公开(公告)日：2020-03-19

申请号：US16618926

申请日：2018-06-12

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Phillip Kim ,  Anthony Atala ,  David James Mccoul

IPC: A61F2/24 ,  G16H50/50 ,  G16H30/40 ,  A61B34/10

Abstract: Provided herein is a method of forming a ventricular support device for a diseased heart, including providing imaging data of the diseased heart, forming a three-dimensional (3D) heart model based on the imaging data, providing strain data including a plurality of strain estimates for at least one segment of the diseased heart, mapping the plurality of strain estimates onto corresponding portions of the 3D heart model to form a 3D diseased heart model, based on the 3D diseased heart model, forming a model of the ventricular support device configured to surround at least a portion of the diseased heart and provide support based upon said strain estimates, and converting the model of the ventricular support device to a digital file useful for directing a 3D printer device to print said ventricular support device for said diseased heart. Also provided is a customized ventricular support device so produced.

公开(公告)号：US09879278B2

公开(公告)日：2018-01-30

申请号：US14382816

申请日：2013-02-27

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Baisong Lu ,  Qingguo Zhao ,  James Yoo ,  Anthony Atala

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N2800/40 ,  C12N2840/007

Abstract: The invention includes compositions and methods for the selective expression of a target gene in a subset of cells. In certain embodiments, the present invention includes a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated by a non-constitutive promoter. The construct is able to maintain episomal state, no matter whether the target gene is expressed in the cell.

公开(公告)号：US09039782B2

公开(公告)日：2015-05-26

申请号：US14256643

申请日：2014-04-18

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  James J. Yoo ,  Grace Lim ,  Sang Jin Lee

IPC: A61F2/28 ,  A61K38/18 ,  A61L27/36 ,  A61L27/38 ,  A61L27/48 ,  A61L27/56 ,  A61L27/58 ,  A61K35/32 ,  A61K35/34

CPC classification number: A61F2/28 ,  A61K35/32 ,  A61K35/34 ,  A61K38/18 ,  A61L27/3604 ,  A61L27/3817 ,  A61L27/3821 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3891 ,  A61L27/48 ,  A61L27/56 ,  A61L27/58 ,  A61L2430/02

Abstract: The invention pertains to methods of producing artificial composite tissue constructs that permit coordinated motion. Biocompatable structural matrices having sufficient rigidity to provide structural support for cartilage-forming cells and bone-forming cells are used. Biocompatable flexible matrices seeded with muscle cells are joined to the structural matrices to produce artificial composite tissue constructs that are capable of coordinated motion.

Abstract translation: 本发明涉及生产允许协调运动的人造复合组织结构的方法。 使用具有足够刚性以提供软骨形成细胞和骨形成细胞的结构支持的生物相容性结构基质。 用肌肉细胞接种的生物相容的柔性基质连接到结构基质上以产生能够协同运动的人造复合组织构建体。

公开(公告)号：US09005972B2

公开(公告)日：2015-04-14

申请号：US14185090

申请日：2014-02-20

Applicant: Wake Forest University Health Sciences

Inventor： Tao Xu ,  James J. Yoo ,  Anthony Atala ,  Dennis Dice

IPC: A61K9/00 ,  B41M5/52 ,  B41M99/00 ,  C12N11/04 ,  A61K35/36 ,  A61L27/38 ,  A61L27/50 ,  A61L27/58 ,  B41J3/407 ,  C12N11/08 ,  D01D5/00 ,  D01F6/62 ,  A61K35/32 ,  A61K35/34 ,  A61F2/06

CPC classification number: A61K9/5089 ,  A61F2/062 ,  A61K35/32 ,  A61K35/34 ,  A61K35/36 ,  A61L27/38 ,  A61L27/50 ,  A61L27/58 ,  B33Y80/00 ,  B41J3/407 ,  C12N5/0655 ,  C12N11/04 ,  C12N11/08 ,  C12N2533/54 ,  C12N2533/56 ,  D01D5/0007 ,  D01F6/625

Abstract: Provided herein is an apparatus for printing cells which includes an electrospinning device and an inkjet printing device operatively associated therewith. Methods of making a biodegradable scaffold having cells seeded therein are also provided. Methods of forming microparticles containing one or more cells encapsulated by a substrate are also provided, as are methods of forming an array of said microparticles.

Abstract translation: 本文提供了一种用于印刷电池的装置，其包括静电纺丝装置和与其可操作地相关联的喷墨打印装置。 还提供了制备具有种子细胞的可生物降解的支架的方法。 还提供了形成包含一个或多个由基底包封的细胞的微粒的方法，以及形成所述微粒阵列的方法。