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16 条记录 (耗时 0.02 秒)

    Polynucleotide sequence assay
    1.
    发明授权
    Polynucleotide sequence assay 有权

    公开(公告)号：US06511810B2

    公开(公告)日：2003-01-28

    申请号：US09898323

    申请日：2001-07-03

    申请人： Wanli Bi ,  Kenneth J. Livak ,  Will Bloch

    发明人： Wanli Bi ,  Kenneth J. Livak ,  Will Bloch

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6862 ,  C12Q2531/137 ,  C12Q2521/319

    摘要： Disclosed are methods for detecting or quantifying one or more target polynucleotide sequences in a sample. In one aspect, a sample is contacted with first and second probe pair that are capable of hybridizing to a selected target sequence and a corresponding complementary sequence, respectively. Probe cleavage and ligation results in the formation of ligation products which can be generated in an exponential fashion when the target sequence and/or complement are present in the sample. In another embodiment, a single probe pair can be used to form ligation product in a linear fashion from a complementary template. Reagents and kits are also disclosed.

    Quantitative Calibration Method and System for Genetic Analysis Instrumentation
    2.
    发明申请
    Quantitative Calibration Method and System for Genetic Analysis Instrumentation 审中-公开
    遗传分析仪器的定量校准方法和系统

    公开(公告)号：US20100276580A1

    公开(公告)日：2010-11-04

    申请号：US12611892

    申请日：2009-11-03

    申请人： Muhammad A. Sharaf ,  Wanli Bi ,  Kenneth J. Livak

    发明人： Muhammad A. Sharaf ,  Wanli Bi ,  Kenneth J. Livak

    IPC分类号： G12B13/00 ,  G01N21/64 ,  G01N21/25

    CPC分类号： G01N21/274 ,  G01N21/278 ,  G01N21/6428 ,  G01N21/6452

    摘要： Aspects of the present invention provide a method and apparatus of generating a calibration matrix for a spectral detector instrument. A calibration plate contains one or more dye mixtures in each well of the calibration plate at known absolute concentration. From the calibration plate, aspects of the present invention are used to prepare a concentration matrix based on the dyes used in the assay and the different dye mixtures used in the calibration plate. An excitation source exposes the calibration plate causing the spectral species in each of the wells to fluoresce. The emission spectra for the different dye mixtures of dyes as gathered by the spectral detector instrument at different points in the range of spectra is used to generate a spectral matrix. Bilinear calibration is performed on the concentration matrix and the spectral matrix as to determine a calibration matrix relating spectra directly to absolute concentrations.

    摘要翻译： 本发明的方面提供了一种生成用于光谱检测器仪器的校准矩阵的方法和装置。 校准板在已知的绝对浓度下在校准板的每个孔中含有一种或多种染料混合物。 根据校准板，本发明的各方面用于制备基于测定中使用的染料和校准板中使用的不同染料混合物的浓度基质。 激发源暴露校准板，导致每个孔中的光谱物质发荧光。 由光谱检测仪器在光谱范围内的不同点收集的不同染料染料混合物的发射光谱用于产生光谱矩阵。 对浓度矩阵和光谱矩阵进行双线性校准，以确定直接将光谱与绝对浓度相关的校准矩阵。

    QUANTITATIVE CALIBRATION METHOD AND SYSTEM FOR GENETIC ANALYSIS INSTRUMENTATION
    3.
    发明申请
    QUANTITATIVE CALIBRATION METHOD AND SYSTEM FOR GENETIC ANALYSIS INSTRUMENTATION 审中-公开
    量化校准方法和遗传分析仪器系统

    公开(公告)号：US20080001099A1

    公开(公告)日：2008-01-03

    申请号：US11428385

    申请日：2006-07-01

    申请人： MUHAMMAD A. SHARAF ,  Wanli Bi ,  Kenneth J. Livak

    发明人： MUHAMMAD A. SHARAF ,  Wanli Bi ,  Kenneth J. Livak

    IPC分类号： G01N21/64

    CPC分类号： G01N21/274 ,  G01N21/278 ,  G01N21/6428 ,  G01N21/6452

    摘要： Aspects of the present invention provide a method and apparatus of generating a calibration matrix for a spectral detector instrument. A calibration plate contains one or more dye mixtures in each well of the calibration plate at known absolute concentration. From the calibration plate, aspects of the present invention are used to prepare a concentration matrix based on the dyes used in the assay and the different dye mixtures used in the calibration plate. An excitation source exposes the calibration plate causing the spectral species in each of the wells to fluoresce. The emission spectra for the different dye mixtures of dyes as gathered by the spectral detector instrument at different points in the range of spectra is used to generate a spectral matrix. Bilinear calibration is performed on the concentration matrix and the spectral matrix as to determine a calibration matrix relating spectra directly to absolute concentrations.

    摘要翻译： 本发明的方面提供了一种生成用于光谱检测器仪器的校准矩阵的方法和装置。 校准板在已知的绝对浓度下在校准板的每个孔中含有一种或多种染料混合物。 根据校准板，本发明的各方面用于制备基于测定中使用的染料和校准板中使用的不同染料混合物的浓度基质。 激发源暴露校准板，导致每个孔中的光谱物质发荧光。 由光谱检测仪器在光谱范围内的不同点收集的不同染料染料混合物的发射光谱用于产生光谱矩阵。 对浓度矩阵和光谱矩阵进行双线性校准，以确定直接将光谱与绝对浓度相关的校准矩阵。

    Reversibly modified thermostable enzyme compositions and methods of making and using the same
    6.
    发明申请
    Reversibly modified thermostable enzyme compositions and methods of making and using the same 审中-公开
    可逆修饰的热稳定性酶组合物及其制备和使用方法

    公开(公告)号：US20060019366A1

    公开(公告)日：2006-01-26

    申请号：US11148946

    申请日：2005-06-08

    申请人： Wanli Bi

    发明人： Wanli Bi

    IPC分类号： C12Q1/68 ,  C12N9/22

    CPC分类号： C12N9/1252 ,  C12N9/1241 ,  C12N9/22 ,  C12N9/99 ,  C12Q1/686

    摘要： The present invention provides reversibly modified thermostable enzyme compositions and methods for making the same. The present invention also provides methods of using the reversibly modified thermostable enzyme compositions, as well as kits and systems comprising the reversibly modified thermostable enzymes.

    摘要翻译： 本发明提供了可逆修饰的热稳定性酶组合物及其制备方法。 本发明还提供了使用可逆修饰的热稳定性酶组合物的方法，以及包含可逆修饰的热稳定酶的试剂盒和系统。

    Nucleic Acid Amplification Using A Reversibly Modified Oligonucleotide
    7.
    发明申请
    Nucleic Acid Amplification Using A Reversibly Modified Oligonucleotide 有权
    使用可逆修饰寡核苷酸的核酸扩增

    公开(公告)号：US20130122507A1

    公开(公告)日：2013-05-16

    申请号：US13682664

    申请日：2012-11-20

    申请人： Wanli Bi

    发明人： Wanli Bi

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C07H21/04 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2525/117 ,  C12Q2525/186 ,  C12Q2527/125

    摘要： The present invention provides a method for amplification of a target nucleic acid sequence or signal, wherein an amplification reaction mixture is used which contains at least one reversibly modified oligonucleotide having a non-hydroxyl group 3′ end which can be converted into a hydroxyl 3′ end upon exposure to a chemical and/or irradiation and/or a range of temperature. The present invention also provides a reversibly modified oligonucleotide as described above, and a nucleic acid amplification reaction mixture and kit comprising such an oligonucleotide.

    摘要翻译： 本发明提供扩增靶核酸序列或信号的方法，其中使用含有至少一个具有非羟基3'末端的可逆修饰寡核苷酸的扩增反应混合物，其可以转化为羟基3' 在暴露于化学和/或照射和/或一定温度范围内结束。 本发明还提供如上所述的可逆修饰的寡核苷酸，以及包含这种寡核苷酸的核酸扩增反应混合物和试剂盒。