公开(公告)号：US20240416259A1

公开(公告)日：2024-12-19

申请号：US18742319

申请日：2024-06-13

Applicant: Waters Technologies Corporation

Inventor： Lavelay Kizekai ,  Michael Dion ,  Stephen Shiner ,  Balasubrahmanyam Addepalli ,  Matthew A. Lauber

IPC: B01D15/34 ,  G01N21/47

Abstract: The present technology provides a description of a flow column dampener device capable of decreasing the MALS noise from solvent contaminants and transient ripples induced by the HPLC pump prior to the evaluation and characterization of the biophysical properties of macromolecules. This dampener typically includes a particle bed column that can trap and remove the noise-inducing soluble particulates from mobile phase, while simultaneously dampening the noise induced by pump-induced ripples. The types of sorbents, sorbent particle size range and column size formats of the device provides significant reduction in MALS noise prior to the evaluation. This device enables confident characterization of cell and gene therapy products with accurate measurements of the physical properties of the macromolecule analytes such as cell and gene therapy products.

公开(公告)号：US20240084279A1

公开(公告)日：2024-03-14

申请号：US18366035

申请日：2023-08-07

Applicant: Waters Technologies Corporation

Inventor： Balasubrahmanyam Addepalli ,  Abraham S. Finny ,  Matthew A. Lauber

IPC: C12N9/50

CPC classification number: C12N9/50 ,  C12Y304/21004 ,  C12Y304/2105 ,  C12Y304/24033

Abstract: This present disclosure relates to engineered protease enzymes, including trypsin, Lys-C and Asp-N proteases, that have enhanced autolysis resistance. Also disclosed herein are methods of using such engineered enzymes for improving detection of target analyte proteins in an analytical assay.

公开(公告)号：US20250033028A1

公开(公告)日：2025-01-30

申请号：US18785487

申请日：2024-07-26

Applicant: Waters Technologies Corporation

Inventor： MingCheng Xu ,  Matthew Lauber ,  Balasubrahmanyam Addepalli ,  Kristine Joy Camacho ,  Oksana Tchoul ,  Lavelay Kizekai

IPC: B01J20/289 ,  B01J20/22 ,  B01J20/28 ,  G01N30/16 ,  G01N30/74

Abstract: The present disclosure is directed to hybrid-bonded siliceous materials. The materials of the present disclosure exhibit enhanced base stability and reduced baseline noise. As such, the materials disclosed herein are suitable for use across a range of chromatography applications, including size-exclusion chromatography and reversed-phase chromatography.

公开(公告)号：US20240401023A1

公开(公告)日：2024-12-05

申请号：US18679628

申请日：2024-05-31

Applicant: Waters Technologies Corporation

Inventor： Mary Elizabeth Trudeau ,  Matthew A. Lauber ,  Nicole Lawrence ,  Balasubrahmanyam Addepalli

IPC: C12N15/10 ,  C12N9/64

Abstract: The present technology is directed to a method and kit for extracting oligonucleotides using solid phase extraction. Oligonucleotides are extracted from a biological sample by using a protease-assisted sample pretreatment step and a subsequent weak anion exchange solid phase extraction process.