公开(公告)号：US20200174008A1

公开(公告)日：2020-06-04

申请号：US16562173

申请日：2019-09-05

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu ,  Matthew Lauber

IPC: G01N33/58 ,  C07D401/12 ,  C07D207/46 ,  C07C261/00 ,  G01N33/533

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.

公开(公告)号：US10436790B2

公开(公告)日：2019-10-08

申请号：US14458760

申请日：2014-08-13

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Ying-Qing Yu ,  Matthew Lauber

IPC: G01N33/00 ,  G01N33/58 ,  C07D401/12 ,  C07D207/46 ,  C07C261/00 ,  G01N33/533

Abstract: Mass spectrometry (MS) active, fluorescent rapid tagging reagent is provided having three substituent groups: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety; and (c) a reactive group that can react with an amine. The reactive group provides rapid tagging of desired bio-molecules. The fluorescent moiety provides the fluorescent signal. The tertiary amino group provides the MS signal.

公开(公告)号：US20240359161A1

公开(公告)日：2024-10-31

申请号：US18647811

申请日：2024-04-26

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Oksana Tchoul ,  Yeliz Tunc Sarisozen ,  MingCheng Xu ,  Matthew Lauber

IPC: B01J20/289 ,  B01D15/22 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/32

CPC classification number: B01J20/289 ,  B01D15/22 ,  B01D15/3809 ,  B01J20/261 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274

Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with streptavidin. The streptavidin functionalized particles of the present disclosure can be bound to biotinylated antibodies, biotinylated antigen-binding fragments thereof, or biotinylated oligonucleotides to create customizable affinity chromatography columns. That is, the functionalized nonporous polymer particles can be packed in a stainless steel, titanium, or other metal or metal alloy column for affinity high pressure liquid chromatography (HPLC).

公开(公告)号：US11994453B2

公开(公告)日：2024-05-28

申请号：US17112408

申请日：2020-12-04

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Matthew Lauber ,  Michael Donegan

IPC: G01N1/40 ,  B01D15/42 ,  B01J20/28 ,  B01J20/281 ,  B01J20/34 ,  G01N30/06 ,  G01N30/16 ,  G01N30/00

CPC classification number: G01N1/405 ,  B01D15/426 ,  B01J20/28052 ,  B01J20/281 ,  B01J20/3475 ,  G01N30/06 ,  G01N30/16 ,  B01J2220/54 ,  G01N2030/009 ,  G01N2030/067

Abstract: The present technology relates to a method for improved recovery of phosphorylated compounds (e.g., phosphorylated glycans). In particular, the present technology utilizes certain chelator additives in a solution to wash and elute the phosphorylated compounds from a solid phase extraction cartridge.

公开(公告)号：US11912983B2

公开(公告)日：2024-02-27

申请号：US17112502

申请日：2020-12-04

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Matthew Lauber ,  Scott Mccall ,  Pamela Iraneta ,  Kerri Smith

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/101 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/315

Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

公开(公告)号：US20220118443A1

公开(公告)日：2022-04-21

申请号：US17502483

申请日：2021-10-15

Applicant: Waters Technologies Corporation

Inventor： Mathew H. DeLano ,  Matthew Lauber ,  Andrew Wyatt Schmudlach ,  Daniel P. Walsh ,  Yeliz Tunc Sarisozen ,  Jonathan Belanger ,  Darryl W. Brousmiche ,  Andrew Bates-Harrison ,  Nicole L. Lawrence

IPC: B01L3/00 ,  C09D143/04

Abstract: The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.

公开(公告)号：US20250033028A1

公开(公告)日：2025-01-30

申请号：US18785487

申请日：2024-07-26

Applicant: Waters Technologies Corporation

Inventor： MingCheng Xu ,  Matthew Lauber ,  Balasubrahmanyam Addepalli ,  Kristine Joy Camacho ,  Oksana Tchoul ,  Lavelay Kizekai

IPC: B01J20/289 ,  B01J20/22 ,  B01J20/28 ,  G01N30/16 ,  G01N30/74

Abstract: The present disclosure is directed to hybrid-bonded siliceous materials. The materials of the present disclosure exhibit enhanced base stability and reduced baseline noise. As such, the materials disclosed herein are suitable for use across a range of chromatography applications, including size-exclusion chromatography and reversed-phase chromatography.

公开(公告)号：US20240425844A1

公开(公告)日：2024-12-26

申请号：US18748243

申请日：2024-06-20

Applicant: Waters Technologies Corporation

Inventor： Szabolcs Fekete ,  Matthew Lauber

IPC: C12N15/10 ,  C12Q1/6806

Abstract: The present technology is directed to methods of improved nucleic acid-based ion-exchange chromatography. In particular, the methods utilize a modification of a sample, and in some examples a modification of mobile phase carrying the sample to the ion-exchange column. In a method of the technology, a bracketed sample is loaded into an injector needle apparatus and injected into an ion-exchange column. The bracketed sample is loaded by (a) adding a first-plug solvent comprising a first salt and having a basic pH; (b) adding a sample plug; and (c) adding a second-plug solvent comprising a second salt and having a basic pH. In certain examples, the bracketed sample is delivered to the column using a mobile phase gradient consisting of first solvent and second solvent and wherein 0% of the second solvent is excluded from the gradient.

公开(公告)号：US20240226845A1

公开(公告)日：2024-07-11

申请号：US18407660

申请日：2024-01-09

Applicant: Waters Technologies Corporation

Inventor： Matthew Lauber ,  Nicole Lawrence ,  Daniel Walsh ,  Jessica Field

IPC: B01J20/10 ,  B01J20/28 ,  B01J20/30 ,  G01N21/33 ,  G01N30/02 ,  G01N30/88

CPC classification number: B01J20/103 ,  B01J20/28016 ,  B01J20/28045 ,  B01J20/3085 ,  G01N21/33 ,  G01N30/88 ,  G01N2030/027 ,  G01N2030/8827

Abstract: Provided herein are compositions, kits and methods that may be useful for improving the efficiency of solid phase extraction to separate oligonucleotides from a complex matrix. Particularly, the present technology relates to a sorbent material including porous particles, the surface of which is modified with a ligand that includes one or more bridging alkyl substituted amines and at least two siloxyl terminal groups.

公开(公告)号：US20210172843A1

公开(公告)日：2021-06-10

申请号：US17112408

申请日：2020-12-04

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Matthew Lauber ,  Michael Donegan

IPC: G01N1/40 ,  B01J20/28 ,  B01J20/34 ,  B01J20/281 ,  B01D15/42 ,  G01N30/16

Abstract: The present technology relates to a method for improved recovery of phosphorylated compounds (e.g., phosphorylated glycans). In particular, the present technology utilizes certain chelator additives in a solution to wash and elute the phosphorylated compounds from a solid phase extraction cartridge.