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    METHODS AND SYSTEMS FOR SEQUENCING LONG NUCLEIC ACIDS
    1.
    发明申请
    METHODS AND SYSTEMS FOR SEQUENCING LONG NUCLEIC ACIDS 审中-公开
    用于测序长核酸的方法和系统

    公开(公告)号：US20140315724A1

    公开(公告)日：2014-10-23

    申请号：US14009089

    申请日：2012-04-02

    申请人： Wei Zhou ,  Rui Mei ,  Filip Crnogorac ,  Guochun Liao ,  Julian Lucas

    发明人： Wei Zhou ,  Rui Mei ,  Filip Crnogorac ,  Guochun Liao ,  Julian Lucas

    IPC分类号： C12Q1/68

    摘要： The present invention provides methods and systems for sequencing long nucleic acid fragments. In one aspect of the invention, methods, systems and reagent kits are provided for sequencing nucleic acid target sequences. Some embodiments of the methods, systems and reagent kits are particularly suitable for sequencing a large number of fragments, particularly long fragments.

    摘要翻译： 本发明提供了用于测序长核酸片段的方法和系统。 在本发明的一个方面，提供了用于测序核酸靶序列的方法，系统和试剂盒。 方法，系统和试剂盒的一些实施方案特别适用于测序大量片段，特别是长片段。

    Methods of targeted sequencing
    6.
    发明授权
    Methods of targeted sequencing 有权

    公开(公告)号：US10072287B2

    公开(公告)日：2018-09-11

    申请号：US13395417

    申请日：2010-09-10

    申请人： Wei Zhou ,  Rui Mei ,  Hajime Matsuzaki

    发明人： Wei Zhou ,  Rui Mei ,  Hajime Matsuzaki

    IPC分类号： C12Q1/6869 ,  C12Q1/6827

    CPC分类号： C12Q1/6827

    摘要： The present invention provides methods for targeted sequencing of polynucleotide. In one aspect, the present invention provides a method of sequencing a target polynucleotide with fewer probes. In another aspect, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.

    METHODS OF TARGETED SEQUENCING
    7.
    发明申请
    METHODS OF TARGETED SEQUENCING 审中-公开
    目标测序方法

    公开(公告)号：US20130045872A1

    公开(公告)日：2013-02-21

    申请号：US13395417

    申请日：2010-09-10

    申请人： Wei Zhou ,  Rui Mei ,  Hajime Matsuzaki

    发明人： Wei Zhou ,  Rui Mei ,  Hajime Matsuzaki

    IPC分类号： C40B20/00

    CPC分类号： C12Q1/6827

    摘要： The present invention provides methods for targeted sequencing of polynucleotide. In one aspect, the present invention provides a method of sequencing a target polynucleotide with fewer probes. In another aspect, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.

    摘要翻译： 本发明提供多核苷酸的靶向测序方法。 一方面，本发明提供了用较少探针测序靶多核苷酸的方法。 另一方面，本发明提供了用较长读数测序靶多核苷酸的方法。 在本发明中还提供了特异性，连接辅助测序/基因分型方法和连接捕获测序方法。 本发明的方法允许核酸的低成本，高通量和准确的测序。

    Tie down ground anchor head
    10.
    发明授权
    Tie down ground anchor head 有权

    公开(公告)号：US11643785B2

    公开(公告)日：2023-05-09

    申请号：US17033152

    申请日：2020-09-25

    申请人： Wei Zhou ,  Alivia Faye Zhou

    发明人： Wei Zhou ,  Alivia Faye Zhou

    IPC分类号： E02D5/80 ,  E04H12/22 ,  E04B1/343

    CPC分类号： E02D5/801 ,  E04B1/34347 ,  E04H12/2223 ,  E02D2200/1671

    摘要： An anchor head mounted to or incorporated into the shaft of conventional tie down stakes and earth anchors. The anchor head eliminates the need to manually drive the earth anchor into the ground and allows the anchor to be placed and removed using conventional powered drill/drivers and hex sockets or wrenches. The anchor head has a hexagonal top section and a hexagonal bottom sectional separated by an integral, central annular shoulder. The top and bottom sections are each configured to have six equilateral side walls and dimensioned to receive two different sizes of wrench or hex socket. The bottom section of the anchor head has a threaded axial bore for receiving the threaded end of the anchor shaft. The bottom section also has a lateral “swage” bore through which a punch is inserted to swage or deform the threads of the shaft to permanently secure the anchor head to the shaft.