Peer discovery in distributed EPC

    公开(公告)号:US11337051B2

    公开(公告)日:2022-05-17

    申请号:US17048847

    申请日:2019-04-12

    摘要: A discovery protocol for use in a mobile telecommunications network where the protocol is used to discover a target core network component of the network. The network comprises base station core network components having at least one Mobile Management Entity (MME), a Serving Gateway (SGW) a Packet Gateway (PGW), an HSS; and a discovery request distributor connected to all other network components. The protocol utilizes the steps of: sending a discovery request from a first core network component to a discovery request distributor, where the discovery request includes a target identifier of a target core network component to be discovered; forwarding the discovery request to all other core network components within the network; receiving a discovery response at the first network component, from an identified target core network component with the defined target identifier and no responses from the other target core network components.

    Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay
    9.
    发明申请
    Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay 审中-公开
    使用特异性细胞色素c氧化酶I(COI)和/或细胞色素b亚序列通过基于PCR的检测法检测哺乳动物生物体的存在的方法

    公开(公告)号:US20080113349A1

    公开(公告)日:2008-05-15

    申请号:US11592117

    申请日:2006-11-03

    IPC分类号: C12Q1/68 C07H21/00 C12Q1/00

    摘要: The present invention relates to a PCR (polymerase chain reaction) based assay that is useful for detecting, identifying, quantitating and analysis of a target nucleic acid (target nucleic acid hereinafter) in a sample. More specifically, the present invention relates to a PCR based assay that can improve accuracy in detecting, identifying, and quantitating contamination in mammalian cell lines using a PCR-based assay of nucleic acid oligonucleotides (oligoprobes) having a sequence expected to be complementary to a target nucleic acid sequence in a sample. More specifically, the sample may contain either cells or nucleic acid isolated from a cell line. The present invention also relates to a detection kit using the PCR-based assay. The invention also involves a method for using specifically produced nucleic acids complementary to specific sequences or populations of different sequences of the cytochrome c oxidase I (COI) and/or cytochrome b, to detect, identify, and quantify specific organisms, groups of organisms, groups of eukaryotic cells or viruses in cells.

    摘要翻译: 本发明涉及可用于检测,鉴定,定量和分析样品中靶核酸(下文中的靶核酸)的基于PCR(聚合酶链式反应)的测定法。 更具体地,本发明涉及基于PCR的测定法,其可以使用具有期望与其互补的序列的核酸寡核苷酸(寡核苷酸)的基于PCR的测定来提高检测,鉴定和定量哺乳动物细胞系中的污染的准确性 靶核酸序列。 更具体地,样品可以含有从细胞系分离的细胞或核酸。 本发明还涉及使用基于PCR的测定法的检测试剂盒。 本发明还涉及使用特异性产生的与细胞色素c氧化酶I(COI)和/或细胞色素b的不同序列的特定序列或群体互补的核酸来检测,鉴定和定量特定生物体,生物体组, 细胞中的真核细胞或病毒组。