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公开(公告)号:US4234564A
公开(公告)日:1980-11-18
申请号:US861809
申请日:1977-12-19
CPC分类号: C07K14/005 , G01N33/5762 , A61K39/00 , C12N2730/10122 , Y10S436/82
摘要: Compositions comprising HB.sub.s Ag and a physiologically acceptable medium are useful as diagnostic and immunologic agents.
摘要翻译: 包含HBsAg和生理学上可接受的培养基的组合物可用作诊断和免疫剂。
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公开(公告)号:US4118477A
公开(公告)日:1978-10-03
申请号:US830291
申请日:1977-09-02
CPC分类号: C07K14/005 , C12N2730/10122
摘要: An improved process for the preparation of hepatitis B antigen by subjecting biological fluid containing heptatitis B surface antigen to isopycanic banding wherein the step gradient is NaBr. Faster and more economical processing is obtained by multiple loading of the step gradient, either NaBr or conventional gradients.
摘要翻译: 通过使含有乙型肝炎表面抗原的生物液体经受等位基带的制备来改善乙肝抗原的改进方法,其中梯度梯度为NaBr。 通过多次加载阶跃梯度(NaBr或常规梯度)可获得更快更经济的加工。
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公开(公告)号:US4102996A
公开(公告)日:1978-07-25
申请号:US789033
申请日:1977-04-20
CPC分类号: C07K14/005 , G01N33/5762 , A61K39/00 , C12N2730/10122 , Y10S435/961
摘要: HB.sub.c Ag is prepared by isolating Dane particles by isopycnic banding of fluid from HB.sub.s Ag positive donors, optionally but preferably pelleting the Dane particles, and then removing the surface antigen by contacting the Dane particles with a nonionic surfactant having from about 15 to about 35 oxyethylene units in the presence of a reducing agent such as mercaptoethanol. The isolated core antigen is used as a diagnostic and immunologic agent.
摘要翻译: HBcAg通过用HBsAg阳性供体的流体的等压条带分离来分离丹烷颗粒,任选但优选地颗粒化丹麦颗粒,然后通过使丹烷颗粒与具有约15至约35个氧化乙烯单元的非离子表面活性剂接触来除去表面抗原 存在还原剂如巯基乙醇。 分离的核心抗原用作诊断和免疫剂。
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公开(公告)号:US4186193A
公开(公告)日:1980-01-29
申请号:US845016
申请日:1977-10-25
CPC分类号: C07K14/005 , C12N2730/10122
摘要: Hepatitis B surface antigen free of gradient-derived added cesium and potassium ions, for example the antigen in an aqueous solution of sodium bromide.
摘要翻译: 乙型肝炎表面抗原不含梯度衍生的添加铯和钾离子,例如抗原在溴化钠水溶液中。
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公开(公告)号:US4129646A
公开(公告)日:1978-12-12
申请号:US784191
申请日:1977-04-04
CPC分类号: C07K14/005 , B01D43/00 , C12N2730/10122
摘要: Biological fluid containing hepatitis B surface antigen is subjected to isopycnic banding with collection of fractions rich in Dane particles. The Dane particles are useful as diagnostic and immunologic agents.
摘要翻译: 含有乙型肝炎表面抗原的生物液体通过收集富含Dane颗粒的级分进行等环条带。 丹麦颗粒可用作诊断和免疫剂。
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公开(公告)号:US4174388A
公开(公告)日:1979-11-13
申请号:US925736
申请日:1978-07-19
CPC分类号: C07K14/005 , C12N2730/10122
摘要: Hepatitis B immune globulin is prepared from individuals who exhibit an increase in hepatitis B surface antibody of at least 2,000 PHA units/ml following immunization with hepatitis B surface antigen.
摘要翻译: 从用乙型肝炎表面抗原免疫后,乙型肝炎表面抗体增加至少2000个PHA单位/ ml的个体制备乙型肝炎免疫球蛋白。
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公开(公告)号:US4039657A
公开(公告)日:1977-08-02
申请号:US676265
申请日:1976-04-12
CPC分类号: A61K39/00
摘要: Sensitizing plasma protein is dissociated from antigen-plasma protein complex by agitating at low temperature under controlled pH.
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公开(公告)号:US4242324A
公开(公告)日:1980-12-30
申请号:US27184
申请日:1979-04-04
CPC分类号: C07K14/005 , C12N2730/10122
摘要: An improved process for the preparation of hepatitis B antigen by subjecting plasma of hepatitis B donors to isopycanic banding wherein the step gradient is NaBr. Faster and more economical processing is obtained by multiple loading of the step gradient, either NaBr or conventional gradients.
摘要翻译: 通过使乙型肝炎供体的血浆经受等位基带,其中梯度梯度为NaBr,用于制备乙型肝炎抗原的改进方法。 通过多次加载阶跃梯度(NaBr或常规梯度)可获得更快更经济的加工。
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公开(公告)号:US4204989A
公开(公告)日:1980-05-27
申请号:US968896
申请日:1978-12-13
IPC分类号: A61K39/395 , A61K39/29 , C07K14/02 , C07K14/10 , C07K14/18 , C07K14/435 , C07K16/00 , G01N33/576 , C07G7/00
CPC分类号: C07K14/005 , G01N33/5761 , C12N2730/10122 , Y10S530/806 , Y10S530/83
摘要: Hepatitis B e antigen or hepatitis B e antibody are separated from human biological fluid by centrifugation in a density gradient and isolated in the 1.04-1.15 g/cc density region.
摘要翻译: 将乙型肝炎e抗原或乙型肝炎e抗体以密度梯度离心从人体生物液体中分离,并在1.04-1.15g / cc密度区域分离。
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公开(公告)号:US4181713A
公开(公告)日:1980-01-01
申请号:US955863
申请日:1978-10-30
CPC分类号: C07K14/005 , C07K16/082 , A61K39/00 , C12N2730/10122 , Y10S436/82 , Y10S530/83
摘要: High purity HB.sub.s Ag is injected into guinea pigs to stimulate formulation of HB.sub.s Ab-positive serum. Gamma globulins containing HB.sub.s Ab are separated from the serum and the globulin fraction containing HB.sub.s Ab is isolated and coupled to a Sepharose gel. HB.sub.s Ag from HB.sub.s Ag-positive plasma is adsorbed on the gel by formation of an HB.sub.s Ab-HB.sub.s Ag complex. HB.sub.s Ag is eluted from the column and dialysed.
摘要翻译: 将高纯度HBsAg注入豚鼠以刺激HBsAb阳性血清的制剂。 将含有HBsAb的γ球蛋白与血清分离,分离含有HBsAb的球蛋白级分并与琼脂糖凝胶偶联。 通过形成HBsAb-HBsAg复合物,将来自HBsAg阳性血浆的HBsAg吸附在凝胶上。 HBsAg从柱上洗脱并透析。
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