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    Purification of hepatitis B surface antigen
    5.
    发明授权
    Purification of hepatitis B surface antigen 失效
    乙型肝炎表面抗原的纯化

    公开(公告)号：US3994870A

    公开(公告)日：1976-11-30

    申请号：US438269

    申请日：1974-01-31

    申请人： A. Robert Neurath ,  Alfred M. Prince ,  Arnold Lippin

    发明人： A. Robert Neurath ,  Alfred M. Prince ,  Arnold Lippin

    IPC分类号： C07K14/02 ,  A61K39/12 ,  C07G7/028 ,  C12K7/00

    CPC分类号： C07K14/005 ,  C12N2730/10122 ,  Y10S436/82 ,  Y10S530/806 ,  Y10S530/826

    摘要： After a polyethylene glycol purification procedure, virus particles, viral components and virus associated particles having concanavalin A binding sites are further purified by subjecting such particles or components to affinity chromatography utilizing insoluble concanavalin A as a chromatography adsorbent and an eluant capable of interacting with concanavalin A to thereby inhibit the binding of the virus particles or components to the insoluble concanavalin A. Specifically, the insoluble concanavalin A may be concanavalin A linked to agarose beads and the eluant may be methyl-.alpha.-D-mannopyranoside. Specifically, these procedures may be utilized to purify hepatitis B antigen (HB Ag) particles.

    摘要翻译： 在聚乙二醇纯化方法之后，通过使用不溶性刀豆蛋白A作为色谱吸附剂和能够与刀豆素A相互作用的洗脱剂进行亲和层析，进一步纯化具有刀豆素A结合位点的病毒颗粒，病毒组分和病毒相关颗粒 从而抑制病毒颗粒或组分与不溶性刀豆蛋白A的结合。具体而言，不溶性刀豆蛋白A可以是与琼脂糖珠连接的刀豆蛋白A，洗脱剂可以是甲基-α-D-吡喃甘露糖苷。 具体来说，这些方法可用于纯化乙型肝炎抗原（HB Ag）颗粒。

    Temperature-sensitive recombinant mutant viruses and a process for producing same
    6.
    发明授权
    Temperature-sensitive recombinant mutant viruses and a process for producing same 失效
    温度敏感型重组突变病毒及其制备方法

    公开(公告)号：US3992522A

    公开(公告)日：1976-11-16

    申请号：US587974

    申请日：1975-06-18

    申请人： Robert M. Chanock ,  Brian R. Murphy

    发明人： Robert M. Chanock ,  Brian R. Murphy

    IPC分类号： A61K39/145 ,  C12N7/04 ,  C12N15/00 ,  A61K39/18 ,  C12K7/00

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  C12N15/00 ,  A61K2039/5254 ,  A61K2039/543 ,  C12N2760/16134 ,  C12N2760/16161 ,  Y10S424/821

    摘要： A method and resulting temperature sensitive (ts) recombinants which may be produced by modifying wild-type influenza A viruses by utilization of chemically mutagenized donor viruses possessing ts lesions and shut off temperatures in the area 37.degree.-38.degree. C. Such a recombinant virus has been produced that contains the surface H3 antigen of influenza A/Hong Kong/1968 (H3N2) virus and a temperature-sensitive (ts) lesion derived from a ts mutant of influenza A/Great Lakes/1965 (H2N2) virus. Additional donor viruses include influenza A/Hong Kong/1968-ts-1[A], influenza A double recombinant 10B, and influenza A/Hong Kong/1968-ts-1[E]. When utilized as a vaccine, these temperature-sensitive recombinant viruses have the facility to develop only mild symptoms, but the infection induces complete resistant to influenzal disease produced by challenge with a virulent wild-type virus. The temperature-sensitive donor mutant is prepared chemically by growth of the virus in the presence of 5-fluorouracil (5-FU).

    摘要翻译： 可以通过利用具有ts病变的化学诱变的供体病毒修饰野生型甲型流感病毒并在37℃-38℃的区域关闭温度来产生的方法和得到的温度敏感（ts）重组体。这样的重组病毒 已经生产出含有流感A /香港/ 1968（H3N2）病毒的表面H3抗原和源自A / Great Lakes / 1965（H2N2）型流感病毒的ts突变体的温度敏感性（ts）病变。 额外的供体病毒包括甲型流感/香港/ 1968-ts-1 [A]，甲型双重重组10B和流感A /香港/ 1968-ts-1 [E]。 当用作疫苗时，这些对温度敏感的重组病毒具有仅发展轻微症状的功能，但感染诱导通过用强毒野生型病毒攻击产生的流感病毒完全抗性。 通过在5-氟尿嘧啶（5-FU）存在下生长病毒来化学地制备温度敏感的供体突变体。