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公开(公告)号:US4412002A
公开(公告)日:1983-10-25
申请号:US352667
申请日:1982-02-26
CPC分类号: C12N7/00 , C12N2770/32051
摘要: Hepatitis A virus is harvested from persistently infected cells, the cells are then trypsinized, replanted and a second harvest of hepatitis A virus is obtained. The cells can be trypsinized and replanted and harvested indefinitely.
摘要翻译: 从持续感染的细胞中收获甲型肝炎病毒,然后将细胞进行胰蛋白酶消化,重新种植,并获得甲型肝炎病毒的第二次收获。 细胞可以被胰蛋白酶消化和再次无限期地收获和收获。
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公开(公告)号:US4273762A
公开(公告)日:1981-06-16
申请号:US99772
申请日:1979-12-03
IPC分类号: C12N7/00 , A61K35/76 , A61K39/00 , A61K39/12 , A61K39/165
CPC分类号: A61K39/165 , A61K39/12 , A61K2039/5254 , C12N2760/18434
摘要: Lyophilization time of live viral compositions is reduced and output per lyophilization unit is increased by lyophilizing a reduced volume of a more concentrated viral composition.
摘要翻译: 减少活体病毒组合物的冻干时间,并通过冻干减少体积的更浓缩的病毒组合物来增加每个冻干单元的产量。
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公开(公告)号:US5024836A
公开(公告)日:1991-06-18
申请号:US423658
申请日:1989-10-19
IPC分类号: A61K39/245 , A61K39/295
CPC分类号: A61K39/245 , A61K39/12 , A61K2039/5254 , A61K2039/70 , A61K39/0015 , C12N2710/16734
摘要: The invention comprises a lyophilized live herpes virus vaccine that comprises from about 0.5% to about 8% moisture.
摘要翻译: 本发明包括冻干的活疱疹病毒疫苗,其包含约0.5%至约8%的水分。
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公开(公告)号:US4337242A
公开(公告)日:1982-06-29
申请号:US242195
申请日:1981-03-10
IPC分类号: A61K39/12 , A61K39/165 , A61K39/13 , A61K39/245
CPC分类号: A61K39/12 , A61K47/183 , A61K47/26 , A61K47/42 , A61K9/0019 , A61K2039/525 , C12N2760/18434
摘要: An improved stabilized liquid live viral vaccine contains a live virus, partially hydrolyzed gelatin, a monosaccharide or disaccharide, a cell culture medium, L-glutamic acid, L-arginine and sufficient physiologically acceptable acidic buffer to maintain the pH at from about 6.0 to about 6.5.
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公开(公告)号:US4288539A
公开(公告)日:1981-09-08
申请号:US16252
申请日:1979-02-28
IPC分类号: C12Q1/04 , C12Q1/12 , G01N33/569 , G01N33/571 , G01N33/00
CPC分类号: C12Q1/12 , C12Q1/04 , G01N33/56911 , G01N33/571 , Y10S435/81
摘要: Automated tests to evaluate the antibody titer of a sample by incubating a test sample, bacteria, and complement, and detecting bacterial growth in the incubated mixture, and to identify bacteria and its titer in a sample by incubating a test sample, bacterial antiserum, and complement, and detecting inhibition of bacterial growth in the incubated mixture. The automated tests lend themselves to diagnostic use and may be provided in the form of diagnostic test kits.
摘要翻译: 通过孵育测试样品,细菌和补体以及检测培养的混合物中的细菌生长来评估样品的抗体滴度的自动化测试,并通过孵育测试样品,细菌抗血清和样品来鉴定样品中的细菌及其滴度 补体,并检测孵育的混合物中细菌生长的抑制。 自动化测试适用于诊断用途,可以以诊断测试工具包的形式提供。
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公开(公告)号:US4147772A
公开(公告)日:1979-04-03
申请号:US880667
申请日:1978-02-23
IPC分类号: A61K39/12 , A61K39/165 , A61K39/20 , A61K39/16 , A61K39/34
CPC分类号: A61K39/165 , A61K39/12 , C12N7/00 , C12N2760/18434 , C12N2760/18451
摘要: A stabilized lyophlized or liquid live viral vaccine contains a live virus, partially hydrolyzed gelatin, a 6-carbon polyhydric alcohol, Medium 199 and acidic buffer.
摘要翻译: 稳定的冻干或液体活病毒疫苗含有活病毒,部分水解明胶,6-碳多元醇,培养基199和酸性缓冲液。
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公开(公告)号:US4338335A
公开(公告)日:1982-07-06
申请号:US173534
申请日:1980-07-30
IPC分类号: A61K39/12 , A61K39/165 , A61K39/13 , A61K39/245
CPC分类号: A61K39/165 , A61K39/12 , C12N7/00 , A61K2039/525 , A61K2039/5254 , C12N2760/18434 , C12N2760/18451
摘要: An improved stabilized liquid live viral vaccine contains a live virus, partially hydrolyzed gelatin, a monosaccharide or disaccharide, a cell culture medium, L-glutamic acid, L-arginine and sufficient physiologically acceptable acidic buffer to maintain the pH at from about 6.0 to about 6.5.
摘要翻译: 改进的稳定的液体活病毒疫苗含有活病毒,部分水解的明胶,单糖或二糖,细胞培养基,L-谷氨酸,L-精氨酸和足够的生理上可接受的酸性缓冲液,以将pH保持在约6.0至约 6.5。
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公开(公告)号:US4301249A
公开(公告)日:1981-11-17
申请号:US171621
申请日:1980-07-23
CPC分类号: A61K39/29 , A61K39/12 , C12N2770/32434 , C12N2770/32451
摘要: Hepatitis A virus (HAV) is produced in high titer from tissue cultures grown on a hollow fiber capillary unit.
摘要翻译: 从生长在中空纤维毛细管单元上的组织培养物以高效价产生甲型肝炎病毒(HAV)。
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公开(公告)号:US4416986A
公开(公告)日:1983-11-22
申请号:US225555
申请日:1981-01-16
CPC分类号: C07K14/005 , C12N2730/10122
摘要: Hepatitis B surface antigen (HBsAg) is produced in vitro in high titer and purity from tissue cultures of cells that shed HBsAg. The cells are grown on hollow fiber capillary units having a molecular weight cut-off of about 10,000.
摘要翻译: 乙型肝炎表面抗原(HBsAg)在体外以高滴度和纯度从组织培养的HBsAg细胞产生。 细胞在具有约10,000分子量截止值的中空纤维毛细管单元上生长。
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公开(公告)号:US4301250A
公开(公告)日:1981-11-17
申请号:US90169
申请日:1979-11-02
CPC分类号: C07K14/005 , C12N2730/10122
摘要: Hepatitis B surface antigen (HB.sub.s Ag) is produced in vitro in high titer and purity from tissue cultures of cells that shed HB.sub.s Ag by growing the cells with a first incubation at elevated temperature followed by a second incubation at a lower elevated temperature.
摘要翻译: 乙型肝炎表面抗原(HBsAg)以高滴度和纯度从细胞的组织培养物中产生,通过在升高的温度下首先孵育然后在较低的升高温度下进行第二次孵育来培养细胞。
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