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1.发明授权Frame-shifting PCR for germline immunoglobulin genes retrieval and antibody engineering 有权用于种系免疫球蛋白基因检索和抗体工程的帧转换PCR公开(公告)号：US07241598B2
公开(公告)日：2007-07-10
申请号：US10880238
申请日：2004-06-29
Applicant: Wing-Tai Cheung , Man Cheng
Inventor： Wing-Tai Cheung , Man Cheng
CPC classification number: C07K16/00 , C07K16/44 , C07K2317/565 , C07K2317/622
Abstract: A method for preparing an antigen-specific antibody by constructing a library of phage-displayed single chain variable fragment of an antibody with a novel frame-shifting PCR is disclosed. Also disclosed is a method for preparing a clone for producing an antigen-specific antibody.
Abstract translation: 公开了一种通过构建抗体的噬菌体展示的单链可变片段文库来制备抗原特异性抗体的方法，其具有新的帧转换PCR。 还公开了制备用于产生抗原特异性抗体的克隆的方法。
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2.发明申请Frame-shifting PCR for germline immunoglobulin genes retrieval and antibody engineering 有权用于种系免疫球蛋白基因检索和抗体工程的帧转换PCR公开(公告)号：US20050287538A1
公开(公告)日：2005-12-29
申请号：US10880238
申请日：2004-06-29
Applicant: Wing-Tai Cheung , Man Cheng
Inventor： Wing-Tai Cheung , Man Cheng
CPC classification number: C07K16/00 , C07K16/44 , C07K2317/565 , C07K2317/622
Abstract: A method for preparing an antigen-specific antibody by constructing a library of phage-displayed single chain variable fragment of an antibody with a novel frame-shifting PCR is disclosed. Also disclosed is a method for preparing a clone for producing an antigen-specific antibody.
Abstract translation: 公开了一种通过构建抗体的噬菌体展示的单链可变片段文库来制备抗原特异性抗体的方法，其具有新的帧转换PCR。 还公开了制备用于产生抗原特异性抗体的克隆的方法。
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3.发明授权公开(公告)号：US08859205B2
公开(公告)日：2014-10-14
申请号：US12683950
申请日：2010-01-07
Applicant: Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
Inventor： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
CPC classification number: C12P19/34 , C07K16/40 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2319/80 , C12N9/1252 , C12Q1/6804 , C12Q1/686 , C12Q2531/113 , C12Q2527/125 , C12Q2521/101
Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.
Abstract translation: 本发明提供了提高核酸扩增反应效率的方法和组合物。 本发明包括显示比野生型聚合酶增加的活性以及降低的外切核酸酶活性的杂合聚合酶。 本发明还包括用于进行核酸合成和扩增反应的方法，组合物和试剂盒，其中引物的非特异性扩增减少。
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公开(公告)号：US08697410B2
公开(公告)日：2014-04-15
申请号：US13371126
申请日：2012-02-10
Applicant: Man Cheng
Inventor： Man Cheng
CPC classification number: C12N9/1241 , C07K2319/80 , C12N9/1252 , C12N9/96 , C12Q1/686
Abstract: Improved Sso7-polymerase conjugate proteins are provided.
Abstract translation: 提供了改进的Sso7聚合酶缀合蛋白。
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公开(公告)号：US20120258460A1
公开(公告)日：2012-10-11
申请号：US13371126
申请日：2012-02-10
Applicant: Man Cheng , Yan Wang , John Sullivan
Inventor： Man Cheng , Yan Wang , John Sullivan
CPC classification number: C12N9/1241 , C07K2319/80 , C12N9/1252 , C12N9/96 , C12Q1/686
Abstract: Improved Sso7-polymerase conjugate proteins are provided
Abstract translation: 提供了改进的Sso7聚合酶缀合蛋白
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Patent Agency Ranking
公开(公告)号：US08916352B2
公开(公告)日：2014-12-23
申请号：US13370604
申请日：2012-02-10
Applicant: Man Cheng
Inventor： Man Cheng
CPC classification number: C12N9/12 , C07K2319/21 , C07K2319/23 , C07K2319/41 , C07K2319/42 , C07K2319/80 , C12N9/1247 , C12N9/1252 , C12N9/127 , C12N9/1276 , C12Q1/6848 , C12Q1/686 , C12Q2521/101 , C12Q2522/101 , C12Q2527/119 , C12Q2527/125 , C12Q2527/137
Abstract: The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.
Abstract translation: 本发明提供了改善核酸扩增特异性的方法，包括用核酸分子与聚合酶-Sso7 DNA结合结构域缀合物和精氨酸，亚精胺或精胺孵育。 本发明还提供了用于提高核酸扩增特异性的反应混合物和试剂盒。
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公开(公告)号：US20130190195A1
公开(公告)日：2013-07-25
申请号：US13555898
申请日：2012-07-23
Applicant: Man Cheng
Inventor： Man Cheng
IPC: C12Q1/68
CPC classification number: C12Q1/6809 , C12Q2523/313 , C12Q2535/125 , C12Q2563/149 , C12Q2565/543
Abstract: Particles that generate electromagnetic spectra upon appropriate illumination and in which the spectra can be converted to codes that reflect differences in the spectra are functionalized with a plurality of identical primer pairs on which bridge amplification can be performed. Using these particles, nucleic acids in a biological sample are amplified and exposed to a label that sends a signal only when bound to double-stranded DNA. The code for each particle is correlated with the primer pairs, and the captured nucleic acids are thus detected and identified. The procedure is useful for multiplex DNA or RNA detection as well as counting of target DNA and RNA molecules in the sample.
Abstract translation: 在适当的照射下产生电磁光谱并且其中光谱可被转换成反映光谱差异的代码的粒子可以用多个相同的引物对进行功能化，在该引物对上可进行桥接扩增。 使用这些颗粒，生物样品中的核酸被扩增并暴露于仅当结合到双链DNA时才发送信号的标签。 每个粒子的代码与引物对相关，因此检测和鉴定所捕获的核酸。 该程序可用于多重DNA或RNA检测以及样品中靶DNA和RNA分子的计数。
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公开(公告)号：US20120258500A1
公开(公告)日：2012-10-11
申请号：US13370604
申请日：2012-02-10
Applicant: Man Cheng
Inventor： Man Cheng
CPC classification number: C12N9/12 , C07K2319/21 , C07K2319/23 , C07K2319/41 , C07K2319/42 , C07K2319/80 , C12N9/1247 , C12N9/1252 , C12N9/127 , C12N9/1276 , C12Q1/6848 , C12Q1/686 , C12Q2521/101 , C12Q2522/101 , C12Q2527/119 , C12Q2527/125 , C12Q2527/137
Abstract: The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.
Abstract translation: 本发明提供了改善核酸扩增特异性的方法，包括用核酸分子与聚合酶-Sso7 DNA结合结构域缀合物和精氨酸，亚精胺或精胺孵育。 本发明还提供了用于提高核酸扩增特异性的反应混合物和试剂盒。
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9.发明申请公开(公告)号：US20110104760A1
公开(公告)日：2011-05-05
申请号：US12683950
申请日：2010-01-07
Applicant: Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
Inventor： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
CPC classification number: C12P19/34 , C07K16/40 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2319/80 , C12N9/1252 , C12Q1/6804 , C12Q1/686 , C12Q2531/113 , C12Q2527/125 , C12Q2521/101
Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.
Abstract translation: 本发明提供了提高核酸扩增反应效率的方法和组合物。 本发明包括显示比野生型聚合酶增加的活性以及降低的外切核酸酶活性的杂合聚合酶。 本发明还包括用于进行核酸合成和扩增反应的方法，组合物和试剂盒，其中引物的非特异性扩增减少。
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公开(公告)号：US20100136559A1
公开(公告)日：2010-06-03
申请号：US12618076
申请日：2009-11-13
Applicant: Steve Okino , Man Cheng
Inventor： Steve Okino , Man Cheng
IPC: C12Q1/68
CPC classification number: C12Q1/68 , C12Q2527/125
Abstract: The present application provides methods and compositions for determining accessibility of a DNA modifying agent in genomic DNA.
Abstract translation: 本申请提供了用于确定DNA修饰剂在基因组DNA中的可及性的方法和组合物。
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