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    NADH Peroxidase for hydrogen peroxide determination
    2.
    发明授权
    NADH Peroxidase for hydrogen peroxide determination 失效
    NADH过氧化物酶测定过氧化氢

    公开(公告)号：US4186052A

    公开(公告)日：1980-01-29

    申请号：US811422

    申请日：1977-06-29

    申请人： Albert Roder ,  Hans Mollering ,  Wolfgang Gruber ,  Klaus Beaucamp ,  Hans Seidel ,  Peter Stahl ,  Detlef von Hoerschelmann

    发明人： Albert Roder ,  Hans Mollering ,  Wolfgang Gruber ,  Klaus Beaucamp ,  Hans Seidel ,  Peter Stahl ,  Detlef von Hoerschelmann

    IPC分类号： C12N9/08 ,  C12Q1/28 ,  G01N33/50 ,  C07G7/02 ,  C12D13/10 ,  G01N31/14

    CPC分类号： C12N9/0065 ,  C12Q1/28

    摘要： A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.Mto hydrogen peroxide of 2.8.times.10.sup.-5 M andto reduced nicotinamide-adenine-dinucleotide of 1.7.times.10.sup.-5 M,measured at 25.degree. C. in 0.2M tris buffer of pH 6.0, containing 0.1M potassium acetate. The peroxidase can be prepared by liberating the enzyme from Streptococcus faecalis ATCC 8043 by digestion or by treatment with a surface-active agent and isolating same from the enzyme solution obtained. Hydrogen peroxide is determined in a simple reaction or in a coupled reaction with a specific oxidase by contacting the said peroxidase with the H.sub.2 O.sub.2 producing reaction mixture at a pH of from 6.0 to 9.0 and measuring the change of extinction as a measure of H.sub.2 O.sub.2 and of the concentration of nicotinamide-adenine-dinucleotide initially present in said reaction mixture.

    摘要翻译： 提供了一种用于还原型烟酰胺 - 腺嘌呤二核苷酸的新型过氧化物酶，其过氧化物酶用过氧化氢氧化还原型烟酰胺 - 腺嘌呤二核苷酸，得到烟酰胺 - 腺嘌呤二核苷酸和水，其特征在于具有2.8x10-5M的过氧化氢的米氏常数KM， 降低了1.7×10-5M的烟酰胺 - 腺嘌呤二核苷酸，在25℃下，在含有0.1M醋酸钾的0.2M Tris缓冲液pH6.0中测定。 过氧化物酶可以通过消化或通过用表面活性剂处理从粪链球菌ATCC 8043中释放酶制备，并从获得的酶溶液中分离。 过氧化氢在简单的反应或与特定氧化酶的偶联反应中通过使所述过氧化物酶与产生H 2 O 2的反应混合物在6.0至9.0的pH下接触并测定作为H 2 O 2和 最初存在于所述反应混合物中的烟酰胺 - 腺嘌呤二核苷酸的浓度。

    Process for the preparation of cholesterol oxidase
    4.
    发明授权
    Process for the preparation of cholesterol oxidase 失效
    胆固醇氧化酶的制备方法

    公开(公告)号：US4008127A

    公开(公告)日：1977-02-15

    申请号：US633997

    申请日：1975-11-20

    申请人： Wolfgang Gruber ,  Hans Ulrich Bergmeyer ,  Michael Nelboeck-Hochstetter ,  Klaus Beaucamp ,  Gunter Holz ,  Johanna Gramsall ,  Gunter Lang

    发明人： Wolfgang Gruber ,  Hans Ulrich Bergmeyer ,  Michael Nelboeck-Hochstetter ,  Klaus Beaucamp ,  Gunter Holz ,  Johanna Gramsall ,  Gunter Lang

    IPC分类号： C07J9/00 ,  C12N9/04 ,  C12D13/10

    CPC分类号： C12N9/0006 ,  Y10S435/822 ,  Y10S435/872

    摘要： Cholesterol oxidase which oxidizes cholesterol with oxygen to give cholestenone and hydrogen peroxide, is prepared by culturing Nocardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyce erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organism, wherein said sterol compound is of the formula ##STR1## in which R.sub.1 and R.sub.2 are hydrogen or together represent a double bond,R.sub.3 and R.sub.4 are hydrogen or together represent a double bond, andR.sub.5 is hydrogen or alkyl containing up to 3 carbon atoms.

    摘要翻译： 首先通过在含蛋白胨的矿物盐介质上培养诺卡氏菌霍奇金菌ATCC 17895，诺卡氏菌诺卡氏菌诺丝诺菌ATCC 4277，诺卡氏菌ATCC 14811或Proactinomyce erythropolis NCIB 9158来制备胆固醇氧化胆固醇以产生胆碱酯酶和过氧化氢的胆固醇氧化酶， 至少一种固醇化合物作为唯一的碳源，然后分离由培养的微生物形成的胆固醇氧化酶，其中所述甾醇化合物具有式（I），其中R 1和R 2是氢或一起代表 双键，R 3和R 4是氢或一起表示双键，并且R 5是氢或含有至多3个碳原子的烷基。

    Activation of cholesterol oxidase for cholesterol assay
    6.
    发明授权
    Activation of cholesterol oxidase for cholesterol assay 失效
    胆固醇氧化酶激活胆固醇测定

    公开(公告)号：US4164448A

    公开(公告)日：1979-08-14

    申请号：US768530

    申请日：1977-02-14

    申请人： Peter Roeschlau ,  Gunter Lang ,  Klaus Beaucamp ,  Erich Bernt ,  Wolfgang Gruber

    发明人： Peter Roeschlau ,  Gunter Lang ,  Klaus Beaucamp ,  Erich Bernt ,  Wolfgang Gruber

    IPC分类号： C12N9/04 ,  C12Q1/60 ,  C07G7/02 ,  G01N31/14

    CPC分类号： C12N9/0006 ,  C12Q1/60

    摘要： Cholesterol oxidase free of unstabilizing residual traces of detergent is activated by adding to the cholesterol oxidase, before use thereof, at least one surface-active compound with lipophilic and hydrophilic properties. Diagnostic agents for determining cholesterol comprising cholesterol oxidase, a hydrogen peroxide detection system, a buffer, and at least one surface-active compound having lipophilic and hydrophilic properties are provided.

    摘要翻译： 没有不稳定残留痕量的洗涤剂的胆固醇氧化酶通过在使用前加入至少一种具有亲油性和亲水性的表面活性化合物来激活胆固醇氧化酶。 提供了用于测定胆固醇的诊断剂，其包含胆固醇氧化酶，过氧化氢检测系统，缓冲液和至少一种具有亲油性和亲水性的表面活性化合物。

    Process and reagent for determining cholesterol
    7.
    发明授权
    Process and reagent for determining cholesterol 失效
    用于测定胆固醇的方法和试剂

    公开(公告)号：US4184921A

    公开(公告)日：1980-01-22

    申请号：US900958

    申请日：1978-04-28

    申请人： Peter Roeschlau ,  Erich Bernt ,  Wolfgang Gruber

    发明人： Peter Roeschlau ,  Erich Bernt ,  Wolfgang Gruber

    IPC分类号： C12Q1/60 ,  G01N31/14

    CPC分类号： C12Q1/60

    摘要： Cholesterol is determined utilizing cholesterol oxidase, and optionally cholesterol esterase to cleave bound cholesterol, in the presence of peroxidase, p-amino-phenazone, a surface-active agent and phenol, the improvement comprising employing an alkanol of up to 4 carbon atoms in the test composition or sample to substantially overcome test error and deviations due to turbidity formation.

    摘要翻译： 在过氧化物酶，对氨基 - 吩噻嗪，表面活性剂和苯酚的存在下，使用胆固醇氧化酶和任选的胆固醇酯酶来切割结合的胆固醇来确定胆固醇，其改进包括使用最多4个碳原子的链烷醇 测试组合物或样品以基本上克服由于浊度形成引起的测试误差和偏差。