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    Reagents and methods for detecting influenza virus proteins
    1.
    发明授权
    Reagents and methods for detecting influenza virus proteins 有权
    用于检测流感病毒蛋白质的试剂和方法

    公开(公告)号：US08926982B2

    公开(公告)日：2015-01-06

    申请号：US13322459

    申请日：2010-05-28

    申请人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

    发明人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

    IPC分类号： A61K39/145 ,  C07K16/10 ,  G01N33/569 ,  G01N33/573 ,  C12N9/24 ,  A61K39/00

    CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/505 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/34 ,  C12N9/2402 ,  C12N2760/16134 ,  C12Y302/01018 ,  G01N33/573 ,  G01N2333/924 ,  G01N2333/9506

    摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

    摘要翻译： 通过大规模序列分析鉴定了来自甲型流感A型神经氨酸酶的两种普遍保守的序列，然后化学修饰并与载体蛋白缀合以产生单特异性和单克隆抗体。 靶向A型神经氨酸酶的N末端的两种抗体和接近酶活性位点的其他序列能够结合所有9种亚型的神经氨酸酶，同时显示出对病毒神经氨酸酶序列的显着特异性，因为没有交叉反应 观察尿囊蛋白。 使用狭缝印迹的NA的定量分析表明抗体可用于疫苗中的NA抗原定量。 这些代表了首次基于抗体的免疫测定可用于NA定量测定。

    REAGENTS AND METHODS FOR DETECTING INFLUENZA VIRUS PROTEINS
    2.
    发明申请
    REAGENTS AND METHODS FOR DETECTING INFLUENZA VIRUS PROTEINS 审中-公开
    用于检测流感病毒蛋白的试剂和方法

    公开(公告)号：US20120141519A1

    公开(公告)日：2012-06-07

    申请号：US13322459

    申请日：2010-05-28

    申请人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

    发明人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

    IPC分类号： A61K39/145 ,  C07K16/10 ,  C07K1/00 ,  A61P31/16 ,  C12P21/08 ,  G01N33/573 ,  C12N9/96 ,  A61P37/04

    CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/505 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/34 ,  C12N9/2402 ,  C12N2760/16134 ,  C12Y302/01018 ,  G01N33/573 ,  G01N2333/924 ,  G01N2333/9506

    摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

    摘要翻译： 通过大规模序列分析鉴定了来自甲型流感A型神经氨酸酶的两种普遍保守的序列，然后化学修饰并与载体蛋白缀合以产生单特异性和单克隆抗体。 靶向A型神经氨酸酶的N末端的两种抗体和接近酶活性位点的其他序列能够结合所有9种亚型的神经氨酸酶，同时显示出对病毒神经氨酸酶序列的显着特异性，因为没有交叉反应 观察尿囊蛋白。 使用狭缝印迹的NA的定量分析表明抗体可用于疫苗中的NA抗原定量。 这些代表了首次基于抗体的免疫测定可用于NA定量测定。

    Reagents and methods for detecting influenza virus proteins
    5.
    发明授权
    Reagents and methods for detecting influenza virus proteins 有权
    用于检测流感病毒蛋白质的试剂和方法

    公开(公告)号：US08669046B2

    公开(公告)日：2014-03-11

    申请号：US12920838

    申请日：2009-03-10

    申请人： Xuguang (Sean) Li ,  Runtao He ,  Gary Van Domselaar

    发明人： Xuguang (Sean) Li ,  Runtao He ,  Gary Van Domselaar

    IPC分类号： C07K16/10 ,  G01N33/569 ,  C07K7/08 ,  C07K17/06 ,  C12Q1/70 ,  A61K39/145

    CPC分类号： C07K16/1018 ,  C07K14/005 ,  C12N2760/16122 ,  G01N2333/11

    摘要： Antibodies that specifically bind to a peptide having an amino acid sequence as found at the N-terminus of the HA2 fusion peptide of the influenza A virus may be raised by inoculating a mammal with a conjugate of the peptide. In one embodiment, the conjugate comprises the peptide linked to a spacer (e.g. 6-aminocaproic acid) and a carrier protein (e.g. KLH). The antibodies may be used as a universal reagent for detecting HA proteins of influenza viruses. The antibodies are useful as versatile reagents for laboratory research and vaccine potency determination, especially in the event of pandemic influenza outbreaks.

    摘要翻译： 可以通过用肽的缀合物接种哺乳动物来提高特异性结合具有在甲型流感病毒的HA2融合肽的N-末端的氨基酸序列的肽的抗体。 在一个实施方案中，缀合物包含连接到间隔基（例如6-氨基己酸）和载体蛋白（例如KLH）的肽。 抗体可以用作检测流感病毒HA蛋白的通用试剂。 抗体可用作实验室研究和疫苗效力测定的多用试剂，特别是在大流行性流感爆发时。

    REAGENTS AND METHODS FOR DETECTING INFLUENZA VIRUS PROTEINS
    6.
    发明申请
    REAGENTS AND METHODS FOR DETECTING INFLUENZA VIRUS PROTEINS 有权
    用于检测流感病毒蛋白的试剂和方法

    公开(公告)号：US20110059472A1

    公开(公告)日：2011-03-10

    申请号：US12920838

    申请日：2009-03-10

    申请人： Xuguang (Sean) Li ,  Runtao He ,  Gary Van Domselaar

    发明人： Xuguang (Sean) Li ,  Runtao He ,  Gary Van Domselaar

    IPC分类号： G01N33/53 ,  C07K16/08 ,  C07K7/08

    CPC分类号： C07K16/1018 ,  C07K14/005 ,  C12N2760/16122 ,  G01N2333/11

    摘要： Antibodies that specifically bind to a peptide having an amino acid sequence as found at the N-terminus of the HA2 fusion peptide of the influenza A virus may be raised by inoculating a mammal with a conjugate of the peptide. In one embodiment, the conjugate comprises the peptide linked to a spacer (e.g. 6-aminocaproic acid) and a carrier protein (e.g. KLH). The antibodies may be used as a universal reagent for detecting HA proteins of influenza viruses. The antibodies are useful as versatile reagents for laboratory research and vaccine potency determination, especially in the event of pandemic influenza outbreaks.

    摘要翻译： 可以通过用肽的缀合物接种哺乳动物来提高特异性结合具有在甲型流感病毒的HA2融合肽的N-末端的氨基酸序列的肽的抗体。 在一个实施方案中，缀合物包含连接到间隔基（例如6-氨基己酸）和载体蛋白（例如KLH）的肽。 抗体可以用作检测流感病毒HA蛋白的通用试剂。 抗体可用作实验室研究和疫苗效力测定的多用试剂，特别是在大流行性流感爆发时。