公开(公告)号：US20240216581A1

公开(公告)日：2024-07-04

申请号：US17911188

申请日：2021-09-17

申请人： SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

发明人： Chuanzhen HUANG ,  Xu HAN ,  Hanlian LIU ,  Peng YAO ,  Hongtao ZHU ,  Bin ZOU ,  Jun WANG

IPC分类号： A61L27/52 ,  A61L27/20 ,  A61L27/22 ,  B33Y10/00 ,  B33Y80/00 ,  C08J3/075

CPC分类号： A61L27/52 ,  A61L27/20 ,  A61L27/222 ,  B33Y10/00 ,  B33Y80/00 ,  C08J3/075 ,  C08J2305/04 ,  C08J2401/28 ,  C08J2405/00 ,  C08J2405/08

摘要： A photocurable composite hydrogel matrix precursor, a preparation method thereof and a scaffold with same. The photocurable composite hydrogel matrix precursor includes gelatin methacrylate, sodium alginate, sodium carboxymethyl cellulose and chondroitin sulfate, where the mass ratio of a photoinitiator to the gelatin methacrylate to the sodium alginate to the sodium carboxymethyl cellulose to the chondroitin sulfate is (0.2-0.3):(8-10):(1-3):(0.6-0.8):(0.05-0.07). By adoption of the precursor, a cell-loaded printing hydrogel scaffold can be obtained through an extrusion-based 3D bio-printing technology, and the scaffold is controllable in form, good in moldability, high in precision, and has good stability; the biocompatibility and bioactivity are high, so that a good growing environment can be provided for fibroblasts; and the preparation process is simple and can be completed within a short time, moreover, the porosity and mechanical performance of the 3D printing hydrogel scaffold can be adjusted by adjusting the raw material ratio.

公开(公告)号：US20230405187A1

公开(公告)日：2023-12-21

申请号：US17939524

申请日：2022-09-07

申请人： YANSHAN UNIVERSITY ,  SHANDONG UNIVERSITY

发明人： Chuanzhen HUANG ,  Xu HAN ,  Hanlian LIU ,  Zhenyu SHI ,  Peng YAO ,  Hongtao ZHU ,  Bin ZOU ,  Dun LIU ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG

IPC分类号： A61L27/52 ,  C08J3/075 ,  C12N5/00 ,  A61L27/26 ,  B33Y70/00 ,  B33Y80/00 ,  B33Y40/00 ,  B33Y10/00

CPC分类号： A61L27/52 ,  C08J3/075 ,  C12N5/0062 ,  A61L27/26 ,  B33Y70/00 ,  B33Y80/00 ,  B33Y40/00 ,  B33Y10/00 ,  C08J2489/04 ,  C08J2301/28 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/78

摘要： A composite hydrogel for light-cured 3D cell-laden printing and a preparation method and application thereof. The composite hydrogel of the present disclosure combines advantages of gelatin methacryloyl, sodium carboxymethylcellulose, hyaluronic acid-glutamic acid polymer, and the like. The provided composite hydrogel for 3D printing has the characteristics of low toxicity, good biocompatibility and adjustable mechanical properties, can provide cells with a three-dimensional living environment, promotes cell adhesion and migration on gradient scaffolds, and is suitable for tissue engineering scaffolds and cell-laden printing of tissues. The printing process of a scaffold is simple and can be completed within a short time, and the porosity and mechanical properties of the 3D printed hydrogel scaffold can be adjusted by adjusting the proportion of HA-Glu and Col in the hydrogel system.