公开(公告)号：US20090181362A1

公开(公告)日：2009-07-16

申请号：US12065469

申请日：2006-08-29

申请人： Yasuo Suzuki ,  Akira Asai ,  Takashi Suzuki ,  Kazuya Hidari ,  Takeomi Murata ,  Taiichi Usui ,  Sou Takeda ,  Kohei Yamada ,  Toshitada Noguchi

发明人： Yasuo Suzuki ,  Akira Asai ,  Takashi Suzuki ,  Kazuya Hidari ,  Takeomi Murata ,  Taiichi Usui ,  Sou Takeda ,  Kohei Yamada ,  Toshitada Noguchi

IPC分类号： C12Q1/70 ,  C07H7/02

CPC分类号： G01N33/548 ,  G01N33/56983

摘要： A method is provided in which the recognition specificity of a virus for a receptor sugar chain can be easily determined with a simple instrument or apparatus. This method for determining the recognition specificity of a virus for a receptor sugar chain or for determining the change in a host infected in accordance with the mutation of virus includes the steps of bringing a sample of the virus into contact with a support having a polymer with sialo-oligosaccharide immobilized on the surface thereof; and assaying the degree of binding therein to determine the recognition specificity of the virus for the receptor sugar chain and to determine the change in a host range. The method is suitable for the surveillance of a virus.

摘要翻译： 提供了一种方法，其中可以用简单的仪器或装置容易地确定病毒对受体糖链的识别特异性。 用于确定病毒对受体糖链的识别特异性或用于根据病毒突变确定受感染宿主的变化的方法包括以下步骤：使病毒样品与具有聚合物的载体接触 唾液酸寡糖固定在其表面上; 并测定其中的结合程度以确定病毒对受体糖链的识别特异性并确定宿主范围的变化。 该方法适用于病毒监控。

公开(公告)号：US20090269734A1

公开(公告)日：2009-10-29

申请号：US12427636

申请日：2009-04-21

申请人： Yasuo SUZUKI ,  Akira Asai ,  Takashi Suzuki ,  Ilpal Jwa ,  Takeomi Murata ,  Taiichi Usui ,  Sou Takeda ,  Kohei Yamada ,  Toshitada Noguchi

发明人： Yasuo SUZUKI ,  Akira Asai ,  Takashi Suzuki ,  Ilpal Jwa ,  Takeomi Murata ,  Taiichi Usui ,  Sou Takeda ,  Kohei Yamada ,  Toshitada Noguchi

IPC分类号： C12Q1/70 ,  C07K9/00 ,  C12P19/18

CPC分类号： G01N33/548 ,  G01N33/56983

摘要： A method in which the recognition specificity of a virus for a receptor sugar chain can be easily determined with a simple instrument or apparatus is provided. In a method for determining the recognition specificity of a virus for a receptor sugar chain or for determining the change in a host infected in accordance with the mutation of virus comprising, a sample of the virus is brought into contact with a support having a polymer with sialo-oligosaccharide immobilized on the surface thereof; and the degree of binding therein is assayed to determine the recognition specificity of said virus for said receptor sugar chain and to determine the change in a host range. The method is suitable for the surveillance of virus.

摘要翻译： 提供了利用简单的仪器或装置容易地确定病毒对受体糖链的识别特异性的方法。 在用于确定受体糖链病毒的识别特异性或根据病毒突变感染的宿主的变化的方法中，使病毒样品与具有聚合物的载体接触， 唾液酸寡糖固定在其表面上; 并测定其中的结合程度以确定所述病毒对所述受体糖链的识别特异性并确定宿主范围的变化。 该方法适用于病毒监控。

公开(公告)号：US20100168366A1

公开(公告)日：2010-07-01

申请号：US12666482

申请日：2008-06-24

申请人： Taiichi Usui ,  Takeomi Murata ,  Takashi Suzuki ,  Ilpal Jwa ,  Yusuke Ohba ,  Tomoki Hamamoto ,  Toshitada Noguchi

发明人： Taiichi Usui ,  Takeomi Murata ,  Takashi Suzuki ,  Ilpal Jwa ,  Yusuke Ohba ,  Tomoki Hamamoto ,  Toshitada Noguchi

IPC分类号： C08F251/00

CPC分类号： C08G69/48 ,  A61K31/7012 ,  A61K47/645 ,  C08G69/04 ,  C08G69/10 ,  C08G69/40

摘要： A polymer containing an N-linked sialo-glycan wherein a sialo-glycan is condensed to a γ-polyglutamic acid using a chemical compound having an amino group on one end and a carboxyl group on another end and represented by the structural formula (I). Formula (I) (In the formula, Z means a hydroxy group or a residue represented by the formula (II), and n represents an integer of 10 or more, with the proviso that any one or more of the Z's is represented by the formula in (II).) Formula (II) (In the formula, X means a hydroxy group or an acetylamino group, Y1 and Y2 mean a hydroxyl group or an N-acetylneuraminic acid residue, L means a hydrocarbon, an m represents 0 or an integer of 1 or 2, with the proviso that Y1 and Y2 are not the same.)

摘要翻译： 一种含有N-连接的唾液酸聚糖的聚合物，其中唾液酸聚糖用一端具有氨基和另一端的羧基并由结构式（I）表示的化合物缩合成γ-聚谷氨酸， 。 式（I）（式中，Z表示羟基或式（II）表示的残基，n表示10以上的整数，条件是Z中的任一个以上可以由 式（II）中，式（II）（式中，X表示羟基或乙酰氨基，Y1和Y2表示羟基或N-乙酰神经氨酸残基，L表示烃，m表示0 或1或2的整数，条件是Y1和Y2不相同。）

公开(公告)号：US08450441B2

公开(公告)日：2013-05-28

申请号：US12666482

申请日：2008-06-24

申请人： Taiichi Usui ,  Takeomi Murata ,  Takashi Suzuki ,  Ilpal Jwa ,  Yusuke Ohba ,  Tomoki Hamamoto ,  Toshitada Noguchi

发明人： Taiichi Usui ,  Takeomi Murata ,  Takashi Suzuki ,  Ilpal Jwa ,  Yusuke Ohba ,  Tomoki Hamamoto ,  Toshitada Noguchi

IPC分类号： C08F251/00 ,  C07H15/06 ,  C12Q1/70

CPC分类号： C08G69/48 ,  A61K31/7012 ,  A61K47/645 ,  C08G69/04 ,  C08G69/10 ,  C08G69/40

摘要： A polymer containing an N-linked sialo-glycan wherein a sialo-glycan is condensed to a γ-polyglutamic acid using a chemical compound having an amino group on one end and a carboxyl group on another end and represented by the structural formula (I). Formula (I) (In the formula, Z means a hydroxy group or a residue represented by the formula (II), and n represents an integer of 10 or more, with the proviso that any one or more of the Z's is represented by the formula in (II).) Formula (II) (In the formula, X means a hydroxy group or an acetylamino group, Y1 and Y2 mean a hydroxyl group or an N-acetylneuraminic acid residue, L means a hydrocarbon, an m represents 0 or an integer of 1 or 2, with the proviso that Y1 and Y2 are not the same.)

摘要翻译： 一种含有N-连接的唾液酸聚糖的聚合物，其中使用一端具有氨基的化合物和另一端的羧基并由结构式（I）表示的唾液酸聚糖与γ-聚谷氨酸缩合， 。 式（I）（式中，Z表示羟基或式（II）表示的残基，n表示10以上的整数，条件是Z中的任一个以上可以由 式（II）中，式（II）（式中，X表示羟基或乙酰氨基，Y1和Y2表示羟基或N-乙酰神经氨酸残基，L表示烃，m表示0 或1或2的整数，条件是Y1和Y2不相同。）

公开(公告)号：US06797496B1

公开(公告)日：2004-09-28

申请号：US10049964

申请日：2002-07-18

申请人： Nozomu Yasutake ,  Shinsuke Miyoshi ,  Taiichi Usui ,  Takeomi Murata ,  Kazuhide Totani

发明人： Nozomu Yasutake ,  Shinsuke Miyoshi ,  Taiichi Usui ,  Takeomi Murata ,  Kazuhide Totani

IPC分类号： C12P1944

CPC分类号： C07H15/04 ,  C12P19/14

摘要： A lactosyl glycoside or an N-acetyllactosaminyl glycoside useful as foods, functional food materials, medicines, and reagents is provided with high yield and inexpensively by using a transfer reaction represented by the following formula in the presence of an enzyme having an activity of cleaving a &bgr;1,4 glucosyl bond: LacA-X+Y→LacA-Y+X, wherein LacA represents lactose or N-acetyllactosaminide; X represents hydrogen (H), a saccharide, a glycoconjugate, or a phenolic compound; and Y represents a compound having an alcoholic hydroxyl group or a phenolic hydroxyl group.

摘要翻译： 通过使用下述式所示的转移反应，在具有活性的酶的存在下，作为食品，功能性食品材料，药物，试剂而使用的乳糖苷或N-乙酰乳糖胺糖苷可以高产率和低成本地提供 β1,4葡糖基键：其中LacA代表乳糖或N-乙酰乳糖胺酰胺; X表示氢（H），糖，糖缀合物或酚类化合物; Y表示具有醇羟基或酚羟基的化合物。