公开(公告)号：US20100168366A1

公开(公告)日：2010-07-01

申请号：US12666482

申请日：2008-06-24

申请人： Taiichi Usui ,  Takeomi Murata ,  Takashi Suzuki ,  Ilpal Jwa ,  Yusuke Ohba ,  Tomoki Hamamoto ,  Toshitada Noguchi

发明人： Taiichi Usui ,  Takeomi Murata ,  Takashi Suzuki ,  Ilpal Jwa ,  Yusuke Ohba ,  Tomoki Hamamoto ,  Toshitada Noguchi

IPC分类号： C08F251/00

CPC分类号： C08G69/48 ,  A61K31/7012 ,  A61K47/645 ,  C08G69/04 ,  C08G69/10 ,  C08G69/40

摘要： A polymer containing an N-linked sialo-glycan wherein a sialo-glycan is condensed to a γ-polyglutamic acid using a chemical compound having an amino group on one end and a carboxyl group on another end and represented by the structural formula (I). Formula (I) (In the formula, Z means a hydroxy group or a residue represented by the formula (II), and n represents an integer of 10 or more, with the proviso that any one or more of the Z's is represented by the formula in (II).) Formula (II) (In the formula, X means a hydroxy group or an acetylamino group, Y1 and Y2 mean a hydroxyl group or an N-acetylneuraminic acid residue, L means a hydrocarbon, an m represents 0 or an integer of 1 or 2, with the proviso that Y1 and Y2 are not the same.)

摘要翻译： 一种含有N-连接的唾液酸聚糖的聚合物，其中唾液酸聚糖用一端具有氨基和另一端的羧基并由结构式（I）表示的化合物缩合成γ-聚谷氨酸， 。 式（I）（式中，Z表示羟基或式（II）表示的残基，n表示10以上的整数，条件是Z中的任一个以上可以由 式（II）中，式（II）（式中，X表示羟基或乙酰氨基，Y1和Y2表示羟基或N-乙酰神经氨酸残基，L表示烃，m表示0 或1或2的整数，条件是Y1和Y2不相同。）

公开(公告)号：US08450441B2

公开(公告)日：2013-05-28

申请号：US12666482

申请日：2008-06-24

申请人： Taiichi Usui ,  Takeomi Murata ,  Takashi Suzuki ,  Ilpal Jwa ,  Yusuke Ohba ,  Tomoki Hamamoto ,  Toshitada Noguchi

发明人： Taiichi Usui ,  Takeomi Murata ,  Takashi Suzuki ,  Ilpal Jwa ,  Yusuke Ohba ,  Tomoki Hamamoto ,  Toshitada Noguchi

IPC分类号： C08F251/00 ,  C07H15/06 ,  C12Q1/70

CPC分类号： C08G69/48 ,  A61K31/7012 ,  A61K47/645 ,  C08G69/04 ,  C08G69/10 ,  C08G69/40

摘要： A polymer containing an N-linked sialo-glycan wherein a sialo-glycan is condensed to a γ-polyglutamic acid using a chemical compound having an amino group on one end and a carboxyl group on another end and represented by the structural formula (I). Formula (I) (In the formula, Z means a hydroxy group or a residue represented by the formula (II), and n represents an integer of 10 or more, with the proviso that any one or more of the Z's is represented by the formula in (II).) Formula (II) (In the formula, X means a hydroxy group or an acetylamino group, Y1 and Y2 mean a hydroxyl group or an N-acetylneuraminic acid residue, L means a hydrocarbon, an m represents 0 or an integer of 1 or 2, with the proviso that Y1 and Y2 are not the same.)

摘要翻译： 一种含有N-连接的唾液酸聚糖的聚合物，其中使用一端具有氨基的化合物和另一端的羧基并由结构式（I）表示的唾液酸聚糖与γ-聚谷氨酸缩合， 。 式（I）（式中，Z表示羟基或式（II）表示的残基，n表示10以上的整数，条件是Z中的任一个以上可以由 式（II）中，式（II）（式中，X表示羟基或乙酰氨基，Y1和Y2表示羟基或N-乙酰神经氨酸残基，L表示烃，m表示0 或1或2的整数，条件是Y1和Y2不相同。）

公开(公告)号：US20050170470A1

公开(公告)日：2005-08-04

申请号：US10500226

申请日：2002-12-20

申请人： Toshitada Noguchi ,  Tomoki Hamamoto ,  Kiyoshi Okuyama ,  Susumu Shibuya

发明人： Toshitada Noguchi ,  Tomoki Hamamoto ,  Kiyoshi Okuyama ,  Susumu Shibuya

IPC分类号： C12P19/40 ,  C07H5/06 ,  C12P19/30

CPC分类号： C12P19/40

摘要： The invention provides a process for producing 2′-deoxyguanosine, characterized in that the process includes reacting one compound selected from the group consisting of guanosine, guanosine 5′-monophosphate, and 2-amino-6-substituted purine with 2′-deoxynucleoside in the presence of nucleoside deoxyribosyl transferase and a hydrolase. According to the process of the present invention, 2′-deoxyguanosine can be synthesized efficiently from inexpensive and easily available starting materials. Since no guanosine, which disturbs purification, is virtually present in a reaction mixture, isolation and purification of 2′-deoxyguanosine can be performed in a very simple manner. Thus, the process for producing 2′-deoxyguanosine is practical.

摘要翻译： 本发明提供了一种生产2'-脱氧鸟苷的方法，其特征在于该方法包括使选自鸟苷，鸟苷5'-单磷酸和2-氨基-6-取代嘌呤的一种化合物与2'-脱氧核苷一起反应 存在核苷脱氧核糖基转移酶和水解酶。 根据本发明的方法，可以从廉价且容易获得的起始材料中有效地合成2'-脱氧鸟苷。 由于干扰净化的鸟苷实际上不存在于反应混合物中，所以可以以非常简单的方式进行2'-脱氧鸟苷的分离和纯化。 因此，生产2'-脱氧鸟苷的方法是实用的。

公开(公告)号：US08148112B2

公开(公告)日：2012-04-03

申请号：US11815329

申请日：2006-02-14

申请人： Shin-Ichiro Nishimura ,  Noriko Nagahori ,  Tomoki Hamamoto ,  Kiyoshi Okuyama ,  Toshitada Noguchi

发明人： Shin-Ichiro Nishimura ,  Noriko Nagahori ,  Tomoki Hamamoto ,  Kiyoshi Okuyama ,  Toshitada Noguchi

IPC分类号： C12P19/50 ,  C12P19/52 ,  C12P19/26 ,  C07H15/00 ,  C07H5/04 ,  C07H5/06 ,  C08B37/00

CPC分类号： C12P19/32 ,  C07H15/08

摘要： The present invention is directed to, for example, an oligosaccharide having at an end thereof a 4-position halogenated galactose residue represented by formula (I): (wherein X represents a halogen atom, and R represents a monosaccharide, an oligosaccharide, or a carrier), a transferase inhibitor containing the oligosaccharide, and a method for inhibiting sugar chain elongation reaction in the presence of glycosyltransferase, the method including employing the inhibitor. The invention also provides a method for producing a 4-position halogenated galactose sugar nucleotide represented by formula (II): (wherein each of R1 to R3 represents a hydroxyl group, an acetyl group, a halogen atom, or a hydrogen atom; X represents a halogen atom; and M represents a hydrogen ion or a metal ion), wherein the method employs bacterium-derived galactokinase and bacterium-derived hexose-1-phosphate uridylyltransferase. The invention is also directed to a sugar chain containing 4-position halogenated galactose envisaged to be employed as drugs and other materials, and to applications of the compound.

摘要翻译： 本发明涉及例如末端具有由式（I）表示的4位卤代半乳糖残基的寡糖：（其中X表示卤素原子，R表示单糖，寡糖或 载体），含有寡糖的转移酶抑制剂，以及在糖基转移酶存在下抑制糖链延长反应的方法，该方法包括使用该抑制剂。 本发明还提供了由式（II）表示的4-位卤代半乳糖糖核苷酸的制备方法：（式中，R 1〜R 3各自表示羟基，乙酰基，卤素原子或氢原子; X表示 卤素原子; M表示氢离子或金属离子），其中该方法采用细菌来源的半乳糖激酶和细菌来源的己糖-1-磷酸尿苷酰转移酶。 本发明还涉及设想用作药物和其它材料的4-位卤代半乳糖的糖链以及该化合物的应用。

公开(公告)号：US08450088B2

公开(公告)日：2013-05-28

申请号：US10573385

申请日：2004-09-21

申请人： Tomoki Hamamoto ,  Kuniaki Nagaoka ,  Toshitada Noguchi

发明人： Tomoki Hamamoto ,  Kuniaki Nagaoka ,  Toshitada Noguchi

IPC分类号： C12P19/02 ,  C12P1/00 ,  C07H1/00 ,  C07H19/10

CPC分类号： C12P19/385 ,  C12P19/305

摘要： A method by which high-purity CMP-N-acetylneuraminic acid (HPLC purity, 95% or higher), which has been difficult to obtain with any technique other than chromatography, can be easily obtained in satisfactory yield by a simple operation without the need of chromatography. The process, which is for producing high-purity CMP-N-acetylneuraminic acid (CMP-NeuAc), is characterized by conducting a suitable combination of the following steps (1) to (4). Step 1: a step in which divalent cations are added to a solution containing CMP-NeuAc to thereby precipitate the phosphoric acid, pyrophosphoric acid, and nucleotide which coexist; Step 2: a step in which a phosphatase is added to a solution containing CMP-NeuAc to thereby convert the coexistent nucleotide into nucleoside; Step 3: a step in which an organic solvent is added to precipitate the CMP-NeuAc; and Step 4: a step in which the CMP-NeuAc precipitated is recovered.

摘要翻译： 可以通过简单的操作容易地以令人满意的产率获得用色谱法以外的任何技术难以获得的高纯度CMP-N-乙酰神经氨酸（HPLC纯度，95％或更高）的方法，而不需要 的色谱。 用于生产高纯度CMP-N-乙酰神经氨酸（CMP-NeuAc）的方法的特征在于进行以下步骤（1）至（4）的合适组合。 步骤1：将二价阳离子加入到含有CMP-NeuAc的溶液中以沉淀磷酸，焦磷酸和共存的核苷酸的步骤; 步骤2：将含有CMP-NeuAc的溶液中加入磷酸酶，从而将共存核苷酸转化为核苷的步骤; 步骤3：加入有机溶剂沉淀CMP-NeuAc的步骤; 步骤4：回收沉淀的CMP-NeuAc的步骤。

公开(公告)号：US20050260718A1

公开(公告)日：2005-11-24

申请号：US10521576

申请日：2003-01-15

申请人： Toshitada Noguchi ,  Tomoki Hamamoto

发明人： Toshitada Noguchi ,  Tomoki Hamamoto

IPC分类号： C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12P19/30 ,  C12P19/18

CPC分类号： C12N9/1241 ,  C12N9/88 ,  C12N9/90 ,  C12P19/305

摘要： The present invention is directed to a process for producing CMP-N-acetylneuraminic acid (CMP-NeuAc), characterized in that the process includes adding yeast cells, N-acetylglucosamine-6-phosphate 2-epimerase (GlcNAc-6P 2-epimerase), N-acetylneuraminic acid lyase (NeuAc lyase), and CMP-N-acetylneuraminic acid synthase (CMP-NeuAc synthase) to a reaction system containing N-acetylglucosamine (GlcNAc), pyruvate, and cytidine 5′-monophosphate (CMP), and inducing reaction of the mixture. The present invention is also directed to a process for producing CMP-N-acetylneuraminic acid (CMP-NeuAc), characterized in that the process includes adding yeast cells, N-acetylglucosamine-6-phosphate 2-epimerase (GlcNAc-6P 2-epimerase), N-acetylneuraminic acid synthase (NeuAc synthase), and CMP-N-acetylneuraminic acid synthase (CMP-NeuAc synthase) to a reaction system containing N-acetylglucosamine (GlcNAc) and cytidine 5′-monophosphate (CMP), and inducing reaction of the mixture.

摘要翻译： 本发明涉及一种生产CMP-N-乙酰神经氨酸（CMP-NeuAc）的方法，其特征在于该方法包括加入酵母细胞，N-乙酰葡糖胺-6-磷酸2-差向异构酶（GlcNAc-6P 2-差向异构酶） ，N-乙酰神经氨酸裂解酶（NeuAc裂解酶）和CMP-N-乙酰神经氨酸合成酶（CMP-NeuAc合成酶）转化成含有N-乙酰葡糖胺（GlcNAc），丙酮酸和胞苷5'-单磷酸（CMP）的反应体系，以及 诱导混合物的反应。 本发明还涉及一种生产CMP-N-乙酰神经氨酸（CMP-NeuAc）的方法，其特征在于该方法包括加入酵母细胞，N-乙酰葡糖胺-6-磷酸2-差向异构酶（GlcNAc-6P 2-差向异构酶 ），N-乙酰神经氨酸合成酶（NeuAc合酶）和CMP-N-乙酰神经氨酸合成酶（CMP-NeuAc合成酶）与含有N-乙酰葡糖胺（GlcNAc）和胞苷5'-单磷酸（CMP）的反应体系，诱导反应 的混合物。

公开(公告)号：US07955825B2

公开(公告)日：2011-06-07

申请号：US10521576

申请日：2003-01-15

申请人： Toshitada Noguchi ,  Tomoki Hamamoto

发明人： Toshitada Noguchi ,  Tomoki Hamamoto

IPC分类号： C12P7/40

CPC分类号： C12N9/1241 ,  C12N9/88 ,  C12N9/90 ,  C12P19/305

摘要： The present invention is directed to a process for producing CMP-N-acetylneuraminic acid (CMP-NeuAc), comprising adding to the cultured E. coli cells which has been transformed with both the DNA encoding N-acetylglucosamine-6-phosphate 2-epimerase (GlcNAc-6P 2-epimerase) and the DNA encoding N-acetylneuraminic acid synthase (NeuAc synthase) and exhibit activities of N-acetylglucosamine-6-phosphate 2-epimerase and N-acetylneuraminic acid synthase, a phosphate buffer containing baker's yeast cells, CMP, N-acetylglucosamine (GlcNAc), magnesium, xylene, glucose, and CMP-N-acetylneuraminic acid synthase (CMP-NeuAc synthase) to provide a reaction mixture, and allowing the reaction to proceed and produce CMP-N-acetylneuraminic acid (CMP-NeuAc), and wherein the process does not require adding ATP.

摘要翻译： 本发明涉及一种生产CMP-N-乙酰神经氨酸（CMP-NeuAc）的方法，其包括将已经用编码N-乙酰葡糖胺-6-磷酸2-差向异构酶的DNA转化的培养的大肠杆菌细胞 （GlcNAc-6P 2-差向异构酶）和编码N-乙酰神经氨酸合酶（NeuAc合酶）的DNA，并显示N-乙酰葡糖胺-6-磷酸2-差向异构酶和N-乙酰神经氨酸合酶（含有面包酵母细胞的磷酸盐缓冲液）的活性， CMP，N-乙酰氨基葡萄糖（GlcNAc），镁，二甲苯，葡萄糖和CMP-N-乙酰神经氨酸合成酶（CMP-NeuAc合成酶）以提供反应混合物，并使反应进行并产生CMP- N-乙酰神经氨酸 CMP-NeuAc），并且其中该方法不需要加入ATP。

公开(公告)号：US20080070285A1

公开(公告)日：2008-03-20

申请号：US10573385

申请日：2004-09-21

申请人： Tomoki Hamamoto ,  Kuniaki Nagaoka ,  Toshitada Noguchi

发明人： Tomoki Hamamoto ,  Kuniaki Nagaoka ,  Toshitada Noguchi

IPC分类号： C12P19/02

CPC分类号： C12P19/385 ,  C12P19/305

摘要： A method by which high-purity CMP-N-acetylneuraminic acid (HPLC purity, 95% or higher), which has been difficult to obtain with any technique other than chromatography, can be easily obtained in satisfactory yield by a simple operation without the need of chromatography. The process, which is for producing high-purity CMP-N-acetylneuraminic acid (CMP-NeuAc), is characterized by conducting a suitable combination of the following steps (1) to (4). Step 1: a step in which divalent cations are added to a solution containing CMP-NeuAc to thereby precipitate the phosphoric acid, pyrophosphoric acid, and nucleotide which coexist; Step 2: a step in which a phosphatase is added to a solution containing CMP-NeuAc to thereby convert the coexistent nucleotide into nucleoside; Step 3: a step in which an organic solvent is added to precipitate the CMP-NeuAc; and Step 4: a step in which the CMP-NeuAc precipitated is recovered.

摘要翻译： 可以通过简单的操作容易地以令人满意的产率获得用色谱法以外的任何技术难以获得的高纯度CMP-N-乙酰神经氨酸（HPLC纯度，95％或更高）的方法，而不需要 的色谱。 用于生产高纯度CMP-N-乙酰神经氨酸（CMP-NeuAc）的方法的特征在于进行以下步骤（1）至（4）的适当组合。 步骤1：将二价阳离子加入到含有CMP-NeuAc的溶液中以沉淀磷酸，焦磷酸和共存的核苷酸的步骤; 步骤2：将含有CMP-NeuAc的溶液中加入磷酸酶，从而将共存核苷酸转化为核苷的步骤; 步骤3：加入有机溶剂沉淀CMP-NeuAc的步骤; 步骤4：回收沉淀的CMP-NeuAc的步骤。

公开(公告)号：US20090018327A1

公开(公告)日：2009-01-15

申请号：US11815329

申请日：2006-02-14

申请人： Shin-Ichiro Nishimura ,  Noriko Nagahori ,  Tomoki Hamamoto ,  Kiyoshi Okuyama ,  Toshitada Noguchi

发明人： Shin-Ichiro Nishimura ,  Noriko Nagahori ,  Tomoki Hamamoto ,  Kiyoshi Okuyama ,  Toshitada Noguchi

IPC分类号： C07H5/02 ,  C12P19/30

CPC分类号： C12P19/32 ,  C07H15/08

摘要： The present invention is directed to, for example, an oligosaccharide having at an end thereof a 4-position halogenated galactose residue represented by formula (I): (wherein X represents a halogen atom, and R represents a monosaccharide, an oligosaccharide, or a carrier), a transferase inhibitor containing the oligosaccharide, and a method for inhibiting sugar chain elongation reaction in the presence of glycosyltransferase, the method including employing the inhibitor.The invention also provides a method for producing a 4-position halogenated galactose sugar nucleotide represented by formula (II): (wherein each of R1 to R3 represents a hydroxyl group, an acetyl group, a halogen atom, or a hydrogen atom; X represents a halogen atom; and M represents a hydrogen ion or a metal ion), wherein the method employs bacterium-derived galactokinase and bacterium-derived hexose-1-phosphate uridylyltransferase.The invention is also directed to a sugar chain containing 4-position halogenated galactose envisaged to be employed as drugs and other materials, and to applications of the compound.

摘要翻译： 本发明涉及例如末端具有由式（I）表示的4-位卤代半乳糖残基的寡糖：（其中，X表示卤素原子，R表示单糖，寡糖，或 载体），含有寡糖的转移酶抑制剂，以及在糖基转移酶存在下抑制糖链延长反应的方法，该方法包括使用该抑制剂。 本发明还提供了由式（II）表示的4-位卤代半乳糖糖核苷酸的制备方法：（式中，R 1〜R 3各自表示羟基，乙酰基，卤素原子或氢原子; X表示 卤素原子; M表示氢离子或金属离子），其中该方法采用细菌来源的半乳糖激酶和细菌来源的己糖-1-磷酸尿苷酰转移酶。 本发明还涉及设想用作药物和其它材料的4-位卤代半乳糖的糖链以及该化合物的应用。

公开(公告)号：US06890739B1

公开(公告)日：2005-05-10

申请号：US10130785

申请日：2000-11-22

申请人： Tomoki Hamamoto ,  Toshitada Noguchi

发明人： Tomoki Hamamoto ,  Toshitada Noguchi

IPC分类号： C12N9/90 ,  C12P19/30 ,  C12P19/38

CPC分类号： C12P19/305 ,  C12N9/90

摘要： The present invention relates to a new use of uridine diphosphate glucose 4-epimerase (also called uridine diphosphate galactose 4-epimerase), and a method of converting uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) to uridine diphosphate N-acetylgalactosamine (UDP-GalNAc) by using the said enzyme. The process for producing UDP-GalNAc by using the uridine diphosphate glucose 4-epimerase and the UDP-GalNAc supply system according to the present invention are practical and efficient, and greatly beneficial to the industries.

摘要翻译： 本发明涉及尿苷二磷酸葡萄糖4-差向异构酶（也称为尿苷二磷酸半乳糖4-差向异构酶）的新用途，以及将尿苷二磷酸N-乙酰葡糖胺（UDP-GlcNAc）转化为尿苷二磷酸N-乙酰半乳糖胺（UDP- GalNAc）。 通过使用本发明的尿苷二磷酸葡萄糖4-差向异构酶和UDP-GalNAc供给系统生产UDP-GalNAc的方法是实用和有效的，对工业有很大的益处。