公开(公告)号：US4931387A

公开(公告)日：1990-06-05

申请号：US714185

申请日：1985-03-20

申请人： Yasuo Yamao ,  Shigeru Fujioka

发明人： Yasuo Yamao ,  Shigeru Fujioka

IPC分类号： C12Q1/00 ,  C12Q1/26 ,  G01N31/22 ,  G01N33/52

CPC分类号： C12Q1/26 ,  G01N33/525 ,  Y10S435/805

摘要： Disclosed is an analysis implement including a support and a reagent layer containing an oxidase applied to the support such that one surface of the reagent layer is exposed to an air atmosphere. A hydrophobic, porous, oxygen permeable layer is positioned between the reagent layer and the support, such that oxygen can permeate to the layer containing oxidase.

摘要翻译： 公开了一种分析工具，其包括载体和试剂层，所述载体和试剂层含有施加到载体的氧化酶，使得试剂层的一个表面暴露于空气气氛。 疏水，多孔，透氧层位于试剂层和载体之间，使得氧气可渗入含有氧化酶的层。

公开(公告)号：US5759866A

公开(公告)日：1998-06-02

申请号：US746779

申请日：1996-11-15

申请人： Koichi Machida ,  Hajime Nakano ,  Masashi Okamoto ,  Tohko Okuyama ,  Shigeru Fujioka

发明人： Koichi Machida ,  Hajime Nakano ,  Masashi Okamoto ,  Tohko Okuyama ,  Shigeru Fujioka

IPC分类号： G01N33/558 ,  G01N33/58 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/58 ,  G01N33/558

摘要： By using an assay device comprising a sample-receiving port, a pump-connection port, a sample-treating zone which is provided with a labeled substance with a label, a sample-treating and optical-measuring zone which has a porous material having immobilized thereto one of a specifically binding pair, and a pathway interconnecting these zones and ports, wherein the sample-treating zone and the sample-treating and optical-measuring zone are positioned between the sample-receiving port and the pump-connection port; or by using an assay device comprising a sample-receiving port, a pump-connection port, a sample-treating and optical-measuring zone which has a porous material having immobilized thereto a labeled substance with a label and one of a specifically binding pair, and a pathway interconnecting these zone and ports, wherein the sample-treating and optical-measuring zone is positioned between the sample-receiving port and the pump-connection port, biological components can be quantitatively determined by immunoassay in a simple manner.

摘要翻译： 通过使用包括样品接收端口，泵连接端口，具有标记物质的样品处理区域的测定装置，具有固定有多孔材料的样品处理和光学测量区域 其中特异性结合对之一和将这些区域和端口相互连接的通路，其中样品处理区和样品处理和光学测量区位于样品接收端口和泵连接端口之间; 或者通过使用包括样品接收端口，泵连接端口，样品处理和光学测量区域的测定装置，其具有固定有标记的标记物和特异性结合对之一的多孔材料， 以及将这些区域和端口互连的路径，其中样品处理和光学测量区域位于样品接收端口和泵连接端口之间，生物组分可以通过免疫测定以简单的方式定量确定。

公开(公告)号：US5717494A

公开(公告)日：1998-02-10

申请号：US747966

申请日：1996-11-12

申请人： Takayuki Taguchi ,  Shigeru Fujioka ,  Tadao Yamaguchi ,  Hisashi Motokawa ,  Atsushi Hosotani ,  Makoto Morishita

发明人： Takayuki Taguchi ,  Shigeru Fujioka ,  Tadao Yamaguchi ,  Hisashi Motokawa ,  Atsushi Hosotani ,  Makoto Morishita

IPC分类号： G01N31/22 ,  G01N21/27 ,  G01N21/31 ,  G01N21/59 ,  G01N21/78 ,  G01N33/52 ,  G01N33/543 ,  G01N21/00

CPC分类号： G01N21/314

摘要： In a method of measuring optical characteristics of a component in a liquid impregnated in a porous material with no affection by the porous material, a light beam having an absorbable wavelength which is absorbed by the component in the liquid and a light beam having a wavelength which is longer than the absorbable wavelength are projected to the porous material, and a quantity of light having the wavelength which is absorbed by the component in the liquid is compensated by a quantity of transmitted light having the wavelength longer than the absorbable wavelength.

摘要翻译： 在不受多孔材料影响的情况下测量浸渍在多孔材料中的液体中的组分的光学特性的方法，具有由液体中的组分吸收的吸收波长的光束和具有波长 比可吸收波长长于多孔材料，并且具有由液体中的成分吸收的波长的光量被波长比可吸收波长长的透射光量补偿。

公开(公告)号：US5764356A

公开(公告)日：1998-06-09

申请号：US747965

申请日：1996-11-12

申请人： Kenichi Iwase ,  Hajime Takeuchi ,  Yuuki Umehara ,  Takayuki Taguchi ,  Shigeru Fujioka ,  Tadao Yamaguchi

发明人： Kenichi Iwase ,  Hajime Takeuchi ,  Yuuki Umehara ,  Takayuki Taguchi ,  Shigeru Fujioka ,  Tadao Yamaguchi

IPC分类号： G01N21/43 ,  G01N21/00 ,  G01N1/10

CPC分类号： G01N21/43

摘要： A method and apparatus for detecting presence of liquid in a pathway of known volume feature a molding with a sample receiving port, a pump connection port, at least one sample treating chamber and optionally at least one optical measuring chamber located between the sample receiving port and the pump connection port. The sample receiving port and the treating chambers are connected with each other through a pathway, which passes through the liquid detecting part, that has at least one air hole that is controlled to be in an open or closed state in response to the presence of liquid detected by the liquid detecting part. A sample is quantitatively fed from the sample receiving port into a sample treating chamber. Detection of the liquid involves projecting a light beam at an incident angle in a range of 42.degree. to 62.degree. with respect to the normal upper surface of the pathway; receiving light transmitted through the pathway or reflected from the upper surface of the pathway; and measuring an intensity of the reflected or transmitted light.

摘要翻译： 用于检测已知体积的通道中的液体存在的方法和装置具有带有样品接收端口的模制件，泵连接端口，至少一个样品处理室和可选地至少一个位于样品接收端口和 泵连接口。 样品接收口和处理室通过通过液体检测部分的路径彼此连接，该通路响应于液体的存在而具有被控制在打开或关闭状态的至少一个空气孔 由液体检测部检测。 将样品从样品接收口定量供给到样品处理室中。 检测液体涉及相对于通路的正常上表面以42°至62°的入射角度投影光束; 接收透过该路径的光或从该路径的上表面反射的光; 并测量反射或透射光的强度。

公开(公告)号：US5681529A

公开(公告)日：1997-10-28

申请号：US518281

申请日：1995-08-23

申请人： Takayuki Taguchi ,  Shigeru Fujioka ,  Koichi Machida ,  Tadao Yamaguchi ,  Hajime Nakano

发明人： Takayuki Taguchi ,  Shigeru Fujioka ,  Koichi Machida ,  Tadao Yamaguchi ,  Hajime Nakano

IPC分类号： B01L3/00 ,  C12Q1/28 ,  C12Q1/54 ,  C12Q1/62 ,  G01N21/03

CPC分类号： B01L3/502 ,  C12Q1/28 ,  C12Q1/54 ,  C12Q1/62 ,  Y10S436/808

摘要： The biological fluid analyzing device for analyzing biological fluid by measuring optical characteristics of a sample has a sample receiving port 1 and a pump connection port 5. Between the sample receiving port 1 and the pump connection port 5, the analyzing device has either a combination of at least one sample-treating chamber 2a, 2b, 2c and at least one optical-measuring chamber 3a, 3b, or a combination of at least one sample-treating chamber, at least one optical-measuring chamber and at least one waste liquid reservoir 4. The sample receiving port 1 is connected with one sample-treating chamber; the pump connection port 5 is connected with a pathway 6 or waste liquid reservoir 4; and the sample-treating chamber and optical-measuring chamber are interconnected with the pathway, or the sample-treating chamber, optical-measuring chamber and waste liquid reservoir are interconnected with the pathway. This construction allows a series of reaction steps to be performed without being affected by physical properties of a liquid sample and thereby assures high level of precision in analyzing the liquid sample. This construction also enables measurements to be made easily.

摘要翻译： 用于通过测量样品的光学特性来分析生物流体的生物流体分析装置具有样品接收口1和泵连接口5.在样品接收口1和泵连接口5之间，分析装置具有 至少一个样品处理室2a，2b，2c和至少一个光学测量室3a，3b或至少一个样品处理室，至少一个光学测量室和至少一个废液储存器 样品接收口1与一个样品处理室连接; 泵连接口5与通道6或废液贮存器4连接; 并且样品处理室和光学测量室与路径相互连接，或者样品处理室，光学测量室和废液储存器与路径相互连接。 这种结构允许在不受液体样品的物理性能影响的情况下进行一系列反应步骤，从而确保分析液体样品的高水平精度。 这种结构也使得能够容易地进行测量。