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1.发明授权公开(公告)号：US07645594B2
公开(公告)日：2010-01-12
申请号：US10803667
申请日：2004-03-18
CPC分类号： G01N1/30 , C12Q1/04 , G01N2001/302 , Y10T436/108331
摘要： A method of staining bacteria comprises: working a polymethine dye on a sample in the presence of a substance capable of reducing nitrite ions to stain bacteria in the sample. A method of detecting bacteria comprises the following steps of: (1) working a polymethine dye on a sample by a method as described above to stain bacteria in the sample, (2) introducing the thus treated sample into a detecting part of a flow cytometer and irradiating cells of the stained bacteria one by one with light to measure scattered light and fluorescent light emitted from each of the cells; and (3) discriminating the bacteria from other components in accordance with an intensity of a scattered light signal and an intensity of a fluorescent light signal or a pulse width reflecting the length of particles to count the bacteria.
摘要翻译： 染色细菌的方法包括：在能够还原亚硝酸根离子的物质存在下在样品上加工聚甲炔染料以染色样品中的细菌。 检测细菌的方法包括以下步骤：（1）通过上述方法在样品上加工聚甲炔染料以染色样品中的细菌，（2）将如此处理的样品引入流式细胞仪的检测部分 用光照射染色细菌的细胞，测量从每个细胞发出的散射光和荧光; 和（3）根据散射光信号的强度和荧光信号的强度或反映粒子长度的脉冲宽度来区别细菌与其它成分，以计数细菌。
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公开(公告)号：US07422870B2
公开(公告)日：2008-09-09
申请号：US10679146
申请日：2003-10-03
IPC分类号： C12Q1/04
摘要： Methods for counting bacteria are described that include: (a) preparing an assay sample by staining a specimen using a fluorescent dye, thereby producing a difference in fluorescent intensity between live bacteria and dead bacteria; (b) detecting optical information from the assay sample; and (c) classifying and counting the live bacteria and the dead bacteria based on the detected optical information. Bacteria counting apparatuses and reagent kits for counting bacteria are also described.
摘要翻译： 描述了计数细菌的方法，包括：（a）通过使用荧光染料染色样品来制备测定样品，从而产生活细菌和死菌之间的荧光强度差异; （b）检测来自测定样品的光学信息; 和（c）根据检测到的光学信息对活细菌和死菌进行分类和计数。 还描述了细菌计数装置和用于计数细菌的试剂盒。
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3.发明授权Method of staining, detection and counting bacteria, and a diluent for bacterial stain 有权染色，检测和计数细菌的方法，以及用于细菌染色的稀释剂公开(公告)号：US07309581B2
公开(公告)日：2007-12-18
申请号：US10005753
申请日：2001-10-29
CPC分类号： G01N1/30 , C12Q1/04 , G01N2001/302 , Y10T436/108331
摘要： A method of staining bacteria comprises: working a polymethine dye on a sample in the presence of a substance capable of reducing nitrite ions to stain bacteria in the sample. A method of detecting bacteria comprises the following steps of: (1) working a polymethine dye on a sample by a method as described above to stain bacteria in the sample, (2) introducing the thus treated sample into a detecting part of a flow cytometer and irradiating cells of the stained bacteria one by one with light to measure scattered light and fluorescent light emitted from each of the cells; and (3) discriminating the bacteria from other components in accordance with an intensity of a scattered light signal and an intensity of a fluorescent light signal or a pulse width reflecting the length of particles to count the bacteria.
摘要翻译： 染色细菌的方法包括：在能够还原亚硝酸根离子的物质存在下在样品上加工聚甲炔染料以染色样品中的细菌。 检测细菌的方法包括以下步骤：（1）通过上述方法在样品上加工聚甲炔染料以染色样品中的细菌，（2）将如此处理的样品引入流式细胞仪的检测部分 用光照射染色细菌的细胞，测量从每个细胞发出的散射光和荧光; 和（3）根据散射光信号的强度和荧光信号的强度或反映粒子长度的脉冲宽度来区别细菌与其它成分，以计数细菌。
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4.发明授权Method, apparatus, reagent kit and reagent for distinguishing erythrocytes in a biological specimen 有权用于区分生物样本中红细胞的方法，装置，试剂盒和试剂公开(公告)号：US07632683B2
公开(公告)日：2009-12-15
申请号：US11241408
申请日：2005-09-30
申请人： Yasuyuki Kawashima , Yasuhiro Sakai
发明人： Yasuyuki Kawashima , Yasuhiro Sakai
CPC分类号： G01N33/80 , G01N15/1459 , G01N33/56961 , Y10T436/101666 , Y10T436/107497
摘要： A method for distinguishing erythrocytes in a biological specimen, includes preparing a sample liquid so as to cause damage to a cell membrane of yeast-like fungi without hemolyzing erythrocytes in a biological specimen and to stain the yeast-like fungi with a fluorescent dye; detecting a first information and a second information from a particle in the sample liquid, wherein the first information reflects a size of the particle and the second information reflects a degree of fluorescent staining of the particle; and distinguishing the erythrocytes from the yeast-like fungi based on the first information and second information detected. An apparatus, a reagent kit and a reagent for carrying out the method are also disclosed.
摘要翻译： 用于区分生物样本中的红细胞的方法包括制备样品液体，以致对酵母样真菌的细胞膜造成伤害，而不会在生物样本中溶血红细胞，并用荧光染料染色酵母样真菌; 从所述样品液体中的颗粒检测第一信息和第二信息，其中所述第一信息反映所述颗粒的尺寸，并且所述第二信息反映所述颗粒的荧光染色程度; 并根据检测到的第一信息和第二信息区分红酵母与酵母样真菌。 还公开了一种用于实施该方法的装置，试剂盒和试剂。
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5.发明申请Method, apparatus, reagent kit and reagent for distinguishing erythrocytes in a biological speciment 有权用于区分生物学中红细胞的方法，装置，试剂盒和试剂公开(公告)号：US20060073601A1
公开(公告)日：2006-04-06
申请号：US11241408
申请日：2005-09-30
申请人： Yasuyuki Kawashima , Yasuhiro Sakai
发明人： Yasuyuki Kawashima , Yasuhiro Sakai
IPC分类号： G01N33/48
CPC分类号： G01N33/80 , G01N15/1459 , G01N33/56961 , Y10T436/101666 , Y10T436/107497
摘要： A method for distinguishing erythrocytes in a biological specimen, comprising the steps of preparing a sample liquid by performing to give a damage to a cell membrane of yeast-like fungi without hemolyzing erythrocytes in a biological specimen and to stain the yeast-like fungi with a fluorescent dye; detecting a first information and a second information from a particle in the sample liquid, wherein the first information reflects a size of the particle and the second information reflects a degree of fluorescent staining of the particle; and distinguishing the erythrocytes from the yeast-like fungi based on the first information and second information detected, is disclosed. An apparatus, a reagent kit and a reagent for carrying out the method are also disclosed.
摘要翻译： 一种用于区分生物样本中的红细胞的方法，包括以下步骤：通过对生物样本中的红细胞进行溶血而对酵母样真菌的细胞膜造成损伤并用酵母样真菌染色来制备样品液体 荧光染料; 从所述样品液体中的颗粒检测第一信息和第二信息，其中所述第一信息反映所述颗粒的尺寸，并且所述第二信息反映所述颗粒的荧光染色程度; 并且基于检测到的第一信息和第二信息来区分红酵母与酵母样真菌。 还公开了一种用于实施该方法的装置，试剂盒和试剂。
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6.发明授权Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same 有权用于稀释血液样本的试剂和通过使用相同的方法测量平均红细胞体积公开(公告)号：US08790929B2
公开(公告)日：2014-07-29
申请号：US12497952
申请日：2009-07-06
申请人： Yusuke Mori , Kinya Uchihashi , Yasuhiro Sakai
发明人： Yusuke Mori , Kinya Uchihashi , Yasuhiro Sakai
IPC分类号： G01N33/48
CPC分类号： G01N1/38 , G01N15/1209 , G01N2015/1236
摘要： The invention provides a reagent for diluting a blood sample, comprising water, polyoxyethylene alkyl ether having a hydroxyl value of 52 to 60, and an osmo-regulator for regulating the osmotic pressure of the reagent in the range of 150 to 400 mOsm/kg, as well as a method for measuring the mean corpuscular volume of a blood sample.
摘要翻译： 本发明提供了一种用于稀释血液样品的试剂，包括水，羟值为52至60的聚氧乙烯烷基醚和用于调节试剂渗透压的渗透压调节剂，范围为150-400mOsm / kg， 以及用于测量血液样品的平均红细胞体积的方法。
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公开(公告)号：US08298622B2
公开(公告)日：2012-10-30
申请号：US11407907
申请日：2006-04-21
IPC分类号： C08F2/48
CPC分类号： B01J19/123 , C01B33/1585
摘要： Methods for producing a silica aerogel coating by: producing a wet gel formed by the hydrolysis and polymerization of an alkoxysilane having an ultraviolet-polymerizable unsaturated group; organically modifying the wet gel with an organic-modifying agent to obtain an organically modified silica having a modification ratio of 10-30% based on a total amount of Si—OH in the wet gel; coating a dispersion of the organically modified silica on a substrate to form a layer; and subjecting the layer of the organically modified silica to ultraviolet irradiation and baking, wherein the silica aerogel coating includes the organically modified silica and wherein the silica aerogel coating has a refractive index in the range of 1.05-1.2.
摘要翻译： 二氧化硅气凝胶涂层的制造方法：制造通过具有紫外线聚合性不饱和基团的烷氧基硅烷的水解和聚合而形成的湿凝胶; 用有机改性剂有机改性湿凝胶，得到基于湿凝胶中Si-OH总量的改性比为10-30％的有机改性二氧化硅; 将有机改性二氧化硅的分散体涂布在基材上以形成层; 并对有机改性二氧化硅层进行紫外线照射和烘烤，其中二氧化硅气凝胶涂层包括有机改性二氧化硅，其中二氧化硅气凝胶涂层的折射率在1.05-1.2范围内。
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公开(公告)号：US20060239886A1
公开(公告)日：2006-10-26
申请号：US11407907
申请日：2006-04-21
IPC分类号： C01B33/12
CPC分类号： B01J19/123 , C01B33/1585
摘要： A method for producing a silica aerogel coating comprising the steps of organically modifying a wet gel formed by the hydrolysis and polymerization of an alkoxysilane having an ultraviolet-polymerizable unsaturated group, and subjecting a layer of the resultant organically modified silica to ultraviolet irradiation and baking.
摘要翻译： 一种二氧化硅气凝胶涂层的制造方法，其特征在于，对通过具有紫外线聚合性不饱和基团的烷氧基硅烷的水解和聚合形成的湿凝胶进行有机改性，并对所得有机改性二氧化硅层进行紫外线照射和烘烤。
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9.发明授权Method and apparatus for preparing resin-impregnated structures reinforced by continuous fibers 失效用于制备由连续纤维增强的树脂浸渍结构的方法和设备
公开(公告)号：US5948473A
公开(公告)日：1999-09-07
申请号：US77596
申请日：1998-05-29
CPC分类号： B29B15/125
摘要： The present invention relates to a method for producing a resin-impregnated structure reinforced by continuous fibers in which a practically endless continuous fiber bundle is dipped in a bath of molten resin and simultaneously passes through a space between a pair of opening pins. The opening pins can be positioned on sides of the bundle so that the continuous fibers are sandwiched by the pins without coming in contact with them. By doing so, the fibers of the bundle can be opened and can be impregnated with the resin. In addition, the foregoing continuous fiber-reinforced resin structure may be cut into pieces having a desired length to provide columnar products.
摘要翻译： PCT No.PCT / JP96 / 03449 Sec。 371日期：1998年5月29日 102（e）日期1998年5月29日PCT 1996年11月25日PCT公布。 WO97 / 19805 PCT公开号 日期：1997年6月5日本发明涉及一种由连续纤维增强的树脂浸渍结构体的制造方法，其中将实际上环状的连续纤维束浸渍在熔融树脂浴中，同时通过一对开口 针脚。 打开销可以位于束的侧面，使得连续纤维被销钉夹持而不与它们接触。 通过这样做，可以打开束的纤维并且可以用树脂浸渍。 此外，上述连续纤维增强树脂结构可以切割成具有期望长度的片以提供柱状产品。
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公开(公告)号：US5670257A
公开(公告)日：1997-09-23
申请号：US568466
申请日：1995-12-07
IPC分类号： C08F30/08 , G02F1/1339 , B32B5/15
CPC分类号： G02F1/1339 , C08F30/08 , Y10T428/2982 , Y10T428/2993
摘要： Organic-inorganic composite particles which include a (meth)acrylic resin framework and a polysiloxane framework having in its molecule an organosilicon containing a silicon atom directly and chemically combined with at least one carbon atom of the (meth)acrylic resin framework, and also contain 25 wt % or more of SiO.sub.2 which constitutes the polysiloxane framework and have a mean particle diameter of .gtoreq.0.5 .mu.m. The particles have the mechanical resilience necessary to maintain a constant gap distance between a pair of members to be arranged with the correct gap distance between them, and the hardness and fracture strength necessary to maintain the gap constant with smaller numbers; and also do not readily cause physical damage to the members. The composite particles can be employed as spacers 8 for a liquid crystal display as they stand or with an adhesive layer formed on the surface. The composite particles can also have a conductive layer formed on the surface, and be employed as conductive particles.
摘要翻译： 包括（甲基）丙烯酸树脂骨架和聚硅氧烷骨架的有机 - 无机复合颗粒，其分子中含有硅原子的有机硅与（甲基）丙烯酸树脂骨架的至少一个碳原子直接化学结合，并且还含有 25重量％以上的构成聚硅氧烷骨架的SiO 2，其平均粒径为≥0.5μm。 颗粒具有必要的机械弹性，以保持一对构件之间的间隙距离与它们之间的正确的间隙距离保持恒定的间隙距离，以及保持间隙恒定的较小数量所需的硬度和断裂强度; 也不会对成员造成身体伤害。 复合颗粒可以用作液晶显示器的间隔件8，或者在表面上形成粘合剂层。 复合颗粒也可以具有在表面上形成的导电层，并且用作导电颗粒。
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