公开(公告)号：US10688330B2

公开(公告)日：2020-06-23

申请号：US15535050

申请日：2015-12-10

Applicant: Yeda Research and Development Co. Ltd. ,  University of Washington

Inventor： Dan S. Tawfik ,  Haim Leader ,  Yaacov Ashani ,  Izhack Cherny ,  Moshe Goldsmith ,  Per Jr. Greisen ,  Sagar D. Khare ,  Gustav Oberdorfer ,  David Baker ,  Sarel Fleishman ,  Adi Goldenzweig ,  Nidhi Aggarwal

IPC: C12N9/16 ,  A61P25/00 ,  A62D3/02 ,  A62D101/02 ,  A62D101/26

Abstract: A genetically modified polypeptide is disclosed which comprises an amino acid sequence of phosphotriesterase (PTE) having at least twice the catalytic efficiency for a V-type nerve agent as a polypeptide which consists of the sequence as set forth in SEQ ID NO: 1, when assayed under identical conditions.

公开(公告)号：US10665324B2

公开(公告)日：2020-05-26

申请号：US15323994

申请日：2015-07-06

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Sarel Fleishman ,  Gideon Lapidoth ,  Maria Gabriele Pszolla ,  Christoffer Norn

IPC: G16B15/00 ,  G16B20/00 ,  G16B35/00 ,  G16C20/60

Abstract: A method for constructing a library of amino-acid sequences having a common structural fold, and a method for designing and selecting an amino-acid sequence having a desired affinity to a molecular surface of interest of a molecular entity, using the library, are provided herein. The methods are based on a stochastic sampling of backbone conformations and amino acid conservation patterns observed in experimentally available protein structures having the common structural fold.

公开(公告)号：US10468119B2

公开(公告)日：2019-11-05

申请号：US15259311

申请日：2016-09-08

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Sarel Fleishman ,  Adi Goldenzweig ,  Dan S. Tawfik

IPC: G16B15/00 ,  C12N9/18 ,  C12N9/16 ,  C12N9/10 ,  G16B30/00 ,  G16B35/00 ,  G16C20/10 ,  G16C20/60 ,  G16B15/20

Abstract: A method for designing and selecting a protein having a stabilized structure compared to a corresponding wild type protein, and proteins having at least six amino acid substitutions with respect to a corresponding wild type protein, designed for improved thermal stability, improved specific activity and/or improved expression levels, are provided herein.

公开(公告)号：US20190083836A1

公开(公告)日：2019-03-21

申请号：US15535050

申请日：2015-12-10

Applicant: Yeda Research and Development Co, Ltd ,  University of Washington

Inventor： Dan S. TAWFIK ,  Haim LEADER ,  Yaacov ASHANI ,  Izhack CHERNY ,  Moshe GOLDSMITH ,  Per Jr. GRIESEN ,  Sagar D KHARE ,  Gustav Oberdorfer ,  David Baker ,  Sarel Fleishman ,  Adi Goldenzweig ,  Nidhi Aggarwal

IPC: A62D3/02 ,  C12N9/16 ,  A61P25/00

CPC classification number: A62D3/02 ,  A61P25/00 ,  A62D2101/02 ,  A62D2101/26 ,  C12N9/16 ,  C12Y301/08001

Abstract: A genetically modified polypeptide is disclosed which comprises an amino acid sequence of phosphotriesterase (PTE) having at least twice the catalytic efficiency for a V-type nerve agent as a polypeptide which consists of the sequence as set forth in SEQ ID NO: 1, when assayed under identical conditions.