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1.发明申请METHODS, CELLS AND SYSTEMS FOR INCORPORATING NON-CANONICAL AMINO ACIDS INTO PROTEINS 有权将非经典氨基酸掺入蛋白质的方法，细胞和系统公开(公告)号：US20120237971A1
公开(公告)日：2012-09-20
申请号：US13353106
申请日：2012-01-18
申请人： Ying Huang , Wenshe Liu , Ying Huang
发明人： Ying Huang , Wenshe Liu , Ying Huang
CPC分类号： C12N15/67
摘要： The present disclosure pertains to methods of incorporating one or more non-canonical amino acids into a protein during a translation of the protein in bacterial cells. The present disclosure also pertains to methods of incorporating one or more non-canonical amino acids into a protein during an in vitro translation of the protein. In additional embodiments, the present disclosure pertains to isolated bacterial cells and in vitro translation systems (e.g., cell-free extract systems) for incorporating one or more non-canonical amino acids into a protein during a translation of the protein.
摘要翻译： 本公开涉及在蛋白质在细菌细胞中翻译期间将一种或多种非标准氨基酸掺入蛋白质的方法。 本公开还涉及在体外翻译蛋白质期间将一种或多种非标准氨基酸掺入蛋白质的方法。 在另外的实施方案中，本公开涉及在翻译蛋白质期间将一种或多种非标准氨基酸掺入到蛋白质中的分离的细菌细胞和体外翻译系统（例如，无细胞提取系统）。
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2.发明授权Methods, cells and systems for incorporating non-canonical amino acids into proteins 有权用于将非标准氨基酸掺入蛋白质的方法，细胞和系统公开(公告)号：US08658394B2
公开(公告)日：2014-02-25
申请号：US13353106
申请日：2012-01-18
申请人： Ying Huang , Wenshe Liu
发明人： Ying Huang , Wenshe Liu
CPC分类号： C12N15/67
摘要： The present disclosure pertains to methods of incorporating one or more non-canonical amino acids into a protein during a translation of the protein in bacterial cells. The present disclosure also pertains to methods of incorporating one or more non-canonical amino acids into a protein during an in vitro translation of the protein. In additional embodiments, the present disclosure pertains to isolated bacterial cells and in vitro translation systems (e.g., cell-free extract systems) for incorporating one or more non-canonical amino acids into a protein during a translation of the protein.
摘要翻译： 本公开涉及在蛋白质在细菌细胞中翻译期间将一种或多种非标准氨基酸掺入蛋白质的方法。 本公开还涉及在体外翻译蛋白质期间将一种或多种非标准氨基酸掺入蛋白质的方法。 在另外的实施方案中，本公开涉及在翻译蛋白质期间将一种或多种非标准氨基酸掺入到蛋白质中的分离的细菌细胞和体外翻译系统（例如，无细胞提取系统）。
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3.发明申请Genetic incorporation of unnatural amino acids into proteins in mammalian cells 有权在哺乳动物细胞中将非天然氨基酸遗传并入蛋白质中公开(公告)号：US20100021963A1
公开(公告)日：2010-01-28
申请号：US12311545
申请日：2007-10-17
申请人： Wenshe Liu , Peter G. Schultz
发明人： Wenshe Liu , Peter G. Schultz
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins in mammalian host cells, for example, primate host cells and rodent host cells. The invention provides, for example but not limited to, translation systems that include host cells (e.g., primate or rodent cells), orthogonal aminoacyl-tRNA synthetases derived from eubacterial synthetases, orthogonal tRNAs, and the unnatural amino acid. The invention also relates to methods for producing proteins of interest comprising at least one unnatural amino acid in mammalian host cell systems.
摘要翻译： 本发明涉及可将哺乳动物宿主细胞（例如灵长类动物宿主细胞和啮齿动物宿主细胞）中的蛋白质中含有非天然氨基酸的tRNA和氨酰-tRNA合成酶的正交对。 本发明提供了例如但不限于包含宿主细胞（例如灵长类或啮齿动物细胞），衍生自真细菌合成酶，正交tRNA和非天然氨基酸的正交氨酰-tRNA合成酶的翻译系统。 本发明还涉及用于在哺乳动物宿主细胞系统中产生包含至少一种非天然氨基酸的目的蛋白质的方法。
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4.发明申请INCORPORATION OF TWO DIFFERENT NONCANONICAL AMINO ACIDS INTO A SINGLE PROTEIN 审中-公开将两种不同的非农药氨基酸纳入单一蛋白质公开(公告)号：US20130078671A1
公开(公告)日：2013-03-28
申请号：US13430552
申请日：2012-03-26
申请人： Wenshe Liu
发明人： Wenshe Liu
摘要： The invention relates to methods, systems, and compositions for the genetic incorporation of a plurality of different noncanonical amino acids into one target protein. The invention provides for multiple, mutually orthogonal aminoacyl-tRNA synthetase/tRNA pairs that suppress two different selector codons engineered into a polynucleotide molecule. By virtue of the suppression of the selector codons, orthogonal aminoacyl-tRNA synthetase/tRNA pairs permit incorporation of their charged noncanonical amino acids into the corresponding positions in the protein. The noncanonical amino acids provide a wide array of functional capabilities. For example, the noncanonical amino acids can provide a reactive pair of moieties that facilitate the study and manipulation of the target protein.
摘要翻译： 本发明涉及用于将多种不同非氨基酸氨基酸遗传并入一种靶蛋白的方法，系统和组合物。 本发明提供多个相互正交的氨基-tRNA合成酶/ tRNA对，其抑制工程化成多核苷酸分子的两个不同的选择密码子。 通过抑制选择密码子，正交氨基-tRNA合成酶/ tRNA对允许将其带电的非氨基酸氨基酸并入蛋白质中的相应位置。 非科学氨基酸提供了广泛的功能性能。 例如，非科学氨基酸可以提供有助于研究和操纵靶蛋白的部分的反应性对。
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公开(公告)号：US09624485B2
公开(公告)日：2017-04-18
申请号：US12311545
申请日：2007-10-17
申请人： Wenshe Liu , Peter G. Schultz
发明人： Wenshe Liu , Peter G. Schultz
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins in mammalian host cells, for example, primate host cells and rodent host cells. The invention provides, for example but not limited to, translation systems that include host cells (e.g., primate or rodent cells), orthogonal aminoacyl-tRNA synthetases derived from eubacterial synthetases, orthogonal tRNAs, and the unnatural amino acid. The invention also relates to methods for producing proteins of interest comprising at least one unnatural amino acid in mammalian host cell systems.
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6.发明申请Genetic incorporation of unnatural amino acids into proteins in mammalian cells 审中-公开在哺乳动物细胞中将非天然氨基酸遗传并入蛋白质中公开(公告)号：US20080166766A1
公开(公告)日：2008-07-10
申请号：US11975351
申请日：2007-10-17
申请人： Wenshe Liu , Peter G. Schultz
发明人： Wenshe Liu , Peter G. Schultz
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins in mammalian host cells, for example, primate host cells and rodent host cells. The invention provides, for example but not limited to, translation systems that include host cells (e.g., primate or rodent cells), orthogonal aminoacyl-tRNA synthetases derived from eubacterial synthetases, orthogonal tRNAs, and the unnatural amino acid. The invention also relates to methods for producing proteins of interest comprising at least one unnatural amino acid in mammalian host cell systems.
摘要翻译： 本发明涉及可将哺乳动物宿主细胞（例如灵长类动物宿主细胞和啮齿动物宿主细胞）中的蛋白质中含有非天然氨基酸的tRNA和氨酰-tRNA合成酶的正交对。 本发明提供了例如但不限于包含宿主细胞（例如灵长类或啮齿动物细胞），衍生自真细菌合成酶，正交tRNA和非天然氨基酸的正交氨酰-tRNA合成酶的翻译系统。 本发明还涉及用于在哺乳动物宿主细胞系统中产生包含至少一种非天然氨基酸的目的蛋白质的方法。
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