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    Flow cytometer and flow cytometry
    4.
    发明申请
    Flow cytometer and flow cytometry 有权
    流式细胞仪和流式细胞术

    公开(公告)号：US20110176127A1

    公开(公告)日：2011-07-21

    申请号：US12923687

    申请日：2010-10-04

    申请人： Masahiko Kanda ,  Shigeaki Kato ,  Sari Fujiyama ,  Kei Izumikawa ,  Toshihiro Chikanishi

    发明人： Masahiko Kanda ,  Shigeaki Kato ,  Sari Fujiyama ,  Kei Izumikawa ,  Toshihiro Chikanishi

    IPC分类号： G01J1/58 ,  G01N21/00 ,  G01J3/30

    CPC分类号： G01N15/1429 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2015/1486

    摘要： In at least one embodiment, a flow cytometer includes a flow cell defining a sheath flow encompassing a dyed biological particle, a first optical source irradiating first light onto the flow cell, a second optical source irradiating second light onto the flow cell downstream where the first light is irradiated, a first optical detector detecting scattered light or fluorescence from the biological particle to output a first electrical signal corresponding thereto, a plurality of second optical detectors arranged along the flow cell, each of the second optical detectors detecting fluorescence from the biological particle to output a second electrical signal corresponding thereto, and a signal processor summing the second electrical signals output from the plurality of the second optical detectors in a plurality of time windows estimated based upon when the first optical detector detects the scattered light or the fluorescence, thereby to increase the second electrical signals of the fluorescence from the biological particle excited by the second light.

    摘要翻译： 在至少一个实施方案中，流式细胞计数器包括限定包围染色的生物颗粒的鞘流的流动池，将第一光照射到流动池上的第一光源，将第二光源照射到下游的流动池， 光被照射，第一光学检测器检测来自生物微粒的散射光或荧光，以输出与其对应的第一电信号，沿着流动池布置的多个第二光学检测器，每个第二光学检测器检测来自生物粒子的荧光 输出与之对应的第二电信号，以及信号处理器，根据第一光检测器何时检测到散射光或荧光估计多个时间窗，对从多个第二光检测器输出的第二电信号相加，从而 增加荧光灯的第二个电信号 从第二个光激发的生物粒子中起泡。

    Devices and methods for detecting and sorting biological particles in a liquid flow using substantially uniform intensity along a direction of flow of a flow cell
    5.
    发明授权
    Devices and methods for detecting and sorting biological particles in a liquid flow using substantially uniform intensity along a direction of flow of a flow cell 有权
    用于使用沿流动池的流动方向基本均匀的强度来检测和分选液体流中的生物颗粒的装置和方法

    公开(公告)号：US08767212B2

    公开(公告)日：2014-07-01

    申请号：US12923687

    申请日：2010-10-04

    申请人： Masahiko Kanda ,  Shigeaki Kato ,  Sari Fujiyama ,  Kei Izumikawa ,  Toshihiro Chikanishi

    发明人： Masahiko Kanda ,  Shigeaki Kato ,  Sari Fujiyama ,  Kei Izumikawa ,  Toshihiro Chikanishi

    IPC分类号： G01N21/00

    CPC分类号： G01N15/1429 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2015/1486

    摘要： In at least one embodiment, a flow cytometer includes a flow cell defining a sheath flow encompassing a dyed biological particle, a first optical source irradiating first light onto the flow cell, a second optical source irradiating second light onto the flow cell downstream where the first light is irradiated, a first optical detector detecting scattered light or fluorescence from the biological particle to output a first electrical signal corresponding thereto, a plurality of second optical detectors arranged along the flow cell, each of the second optical detectors detecting fluorescence from the biological particle to output a second electrical signal corresponding thereto, and a signal processor summing the second electrical signals output from the plurality of the second optical detectors in a plurality of time windows estimated based upon when the first optical detector detects the scattered light or the fluorescence, thereby to increase the second electrical signals of the fluorescence from the biological particle excited by the second light.

    摘要翻译： 在至少一个实施方案中，流式细胞计数器包括限定包围染色的生物颗粒的鞘流的流动池，将第一光照射到流动池上的第一光源，将第二光源照射到下游的流动池， 光被照射，第一光学检测器检测来自生物微粒的散射光或荧光，以输出与其对应的第一电信号，沿着流动池布置的多个第二光学检测器，每个第二光学检测器检测来自生物粒子的荧光 输出与之对应的第二电信号，以及信号处理器，根据第一光检测器何时检测到散射光或荧光估计多个时间窗，对从多个第二光检测器输出的第二电信号相加，从而 增加荧光灯的第二个电信号 从第二个光激发的生物粒子中起泡。