公开(公告)号：US20070121200A1

公开(公告)日：2007-05-31

申请号：US11655793

申请日：2007-01-18

申请人： Yoshimasa Suzuki ,  Kayuri Muraki

发明人： Yoshimasa Suzuki ,  Kayuri Muraki

IPC分类号： G02B21/00

CPC分类号： G01N21/6458 ,  G01N21/6452 ,  G01N21/6486 ,  G02B21/002 ,  G02B21/367

摘要： A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

公开(公告)号：US20070121199A1

公开(公告)日：2007-05-31

申请号：US11655512

申请日：2007-01-18

申请人： Yoshimasa Suzuki ,  Kayuri Muraki

发明人： Yoshimasa Suzuki ,  Kayuri Muraki

IPC分类号： G02B21/00

CPC分类号： G01N21/6458 ,  G01N21/6452 ,  G01N21/6486 ,  G02B21/002 ,  G02B21/367

摘要： A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

公开(公告)号：US20060000962A1

公开(公告)日：2006-01-05

申请号：US11149691

申请日：2005-06-10

申请人： Hiroyuki Imabayashi ,  Kayuri Muraki ,  Yoshimasa Suzuki

发明人： Hiroyuki Imabayashi ,  Kayuri Muraki ,  Yoshimasa Suzuki

IPC分类号： G02B27/40

CPC分类号： G02B21/365 ,  G02B21/245

摘要： An object is to provide a biological sample observation system wherein light damage to the biological sample is decreased, and observation can be performed accurately and quickly over a long period of time. A biological sample observation system which continuously obtains information on a biological sample that is cultured inside of a culturing container has: an imaging section which observes mutually different regions that are previously selected, among regions to be observed including the biological sample, through an object lens for observing the biological sample in the culturing container through a part of the culturing container; an autofocus section which detects the focusing of the object lens with respect to a predetermined region among the regions to be observed; and a focusing drive control section which controls the focusing of the object lens when the biological sample is observed using the imaging section, based on the detection result of the focusing previously performed by the autofocus section. After the focus detection is performed by the autofocus section, without being intervened by the focus detection using the autofocus section, and the different regions are continuously observed by the imaging section, with the focusing controlled by the focusing drive control section.

摘要翻译： 本发明的目的是提供一种生物样品观察系统，其中对生物样品的光损伤降低，并且可以在长时间内精确而快速地进行观察。 连续获取培养容器内培养的生物样品的信息的生物样本观察系统具有：成像部，其通过物镜观察包含生物体样本的待观察区域中预先选择的相互不同的区域 用于通过培养容器的一部分观察培养容器中的生物样品; 自动对焦部，其检测所述物镜相对于要观察的区域中的预定区域的聚焦; 以及聚焦驱动控制部，其基于由所述自动聚焦部先前执行的聚焦的检测结果，使用所述摄像部来观察所述生物样本时的所述物镜的聚焦。 在通过自动聚焦部执行焦点检测之后，不用通过使用自动聚焦部分的焦点检测进行干预，并且由成像部分连续观察不同的区域，聚焦由聚焦驱动控制部分控制。

公开(公告)号：US20070121198A1

公开(公告)日：2007-05-31

申请号：US11655507

申请日：2007-01-18

申请人： Yoshimasa Suzuki ,  Kayuri Muraki

发明人： Yoshimasa Suzuki ,  Kayuri Muraki

IPC分类号： G02B21/00

CPC分类号： G01N21/6458 ,  G01N21/6452 ,  G01N21/6486 ,  G02B21/002 ,  G02B21/367

摘要： A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

公开(公告)号：US20060018013A1

公开(公告)日：2006-01-26

申请号：US11173020

申请日：2005-07-05

申请人： Yoshimasa Suzuki ,  Kayuri Muraki

发明人： Yoshimasa Suzuki ,  Kayuri Muraki

IPC分类号： G02B21/00

CPC分类号： G01N21/6458 ,  G01N21/6452 ,  G01N21/6486 ,  G02B21/002 ,  G02B21/367

摘要： A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

摘要翻译： 提供了即使对于具有明显的亮度不均匀的检查对象也能够精确地进行测量的显微镜成像装置和生物样本检查系统。 显微镜成像装置包括：保持检查对象的台，对被检查物体进行照明的照明单元，拍摄被检查物体的图像的摄像单元，以及移动台和图像摄影装置的移动单元， 采集单元相对于彼此。 图像采集单元包括能够使用时间延迟积分方法进行图像采集的成像装置。 当获取检查对象的多个图像时，对于每个所获取的图像，在成像装置中产生累积电荷的曝光时间被变化，并且将多个图像组合成单个图像。

公开(公告)号：US07271952B2

公开(公告)日：2007-09-18

申请号：US11655512

申请日：2007-01-18

申请人： Yoshimasa Suzuki ,  Kayuri Muraki

发明人： Yoshimasa Suzuki ,  Kayuri Muraki

IPC分类号： G02B21/00

CPC分类号： G01N21/6458 ,  G01N21/6452 ,  G01N21/6486 ,  G02B21/002 ,  G02B21/367

摘要： A microscope imaging apparatus and a biological-specimen examination system that can accurately carry out measurement even for an object under examination having substantial brightness non-uniformity are provided. The microscope imaging apparatus includes a stage that holds the object under examination, an illumination unit that illuminates the object under examination, an image-acquisition unit that acquires images of the object under examination, and a motion unit that moves the stage and the image-acquisition unit relative to each other. The image-acquisition unit includes an imaging device capable of image acquisition using a time delay integration method. When acquiring a plurality of images of the object under examination, the exposure time during which accumulated charge is produced in the imaging device is made different for each of the acquired images, and the plurality of images are combined into a single image.

摘要翻译： 提供了即使对于具有明显的亮度不均匀的检查对象也能够精确地进行测量的显微镜成像装置和生物样本检查系统。 显微镜成像装置包括：保持检查对象的台，对被检查物体进行照明的照明单元，拍摄被检查物体的图像的摄像单元，以及移动台和图像摄影装置的移动单元， 采集单元相对于彼此。 图像采集单元包括能够使用时间延迟积分方法进行图像采集的成像装置。 当获取检查对象的多个图像时，对于每个所获取的图像，在成像装置中产生累积电荷的曝光时间被变化，并且将多个图像组合成单个图像。

公开(公告)号：US08198592B2

公开(公告)日：2012-06-12

申请号：US12360178

申请日：2009-01-27

申请人： Yoshimasa Suzuki ,  Mitsuru Namiki

发明人： Yoshimasa Suzuki ,  Mitsuru Namiki

IPC分类号： G01N21/64

CPC分类号： G01N21/645 ,  G01N2021/0346 ,  G01N2021/6469 ,  G01N2201/0636

摘要： A measuring instrument has a light source for irradiating light including rays of light having the wavelength of excitation light, an objective lens for focusing light irradiated from the light source to a predetermined focusing position, a first mirror for directly reflecting light from the objective lens, a second mirror for reflecting light reflected by the first mirror, the second mirror having an aperture P, and a measuring device for measuring light generated from a sample and having a wavelength different from the wavelength of excitation light, and the sample being arranged between the first mirror and the second mirror, the focusing position of the objective lens being made to agree with the position of the aperture P, and the measuring device being adapted to measure light of a wavelength different from the wavelength of excitation light generated from the sample and passing through the aperture P.

摘要翻译： 测量仪器具有用于照射包括具有激发光波长的光的光的光源，用于将从光源照射的光聚焦到预定聚焦位置的物镜，用于直接反射来自物镜的光的第一反射镜， 用于反射由第一反射镜反射的光的第二反射镜，具有孔径P的第二反射镜，以及用于测量从样品产生的并具有与激发光的波长不同的波长的光的测量装置，并且样品布置在 第一反射镜和第二反射镜，使物镜的聚焦位置与孔P的位置一致，并且测量装置适于测量不同于从样品产生的激发光的波长的波长的光，以及 通过孔径P.

公开(公告)号：US07681439B2

公开(公告)日：2010-03-23

申请号：US11892365

申请日：2007-08-22

申请人： Kazuhiko Kawasaki ,  Satoshi Koga ,  Yoshimasa Suzuki

发明人： Kazuhiko Kawasaki ,  Satoshi Koga ,  Yoshimasa Suzuki

IPC分类号： G01B21/30

CPC分类号： G01Q20/02

摘要： A measuring apparatus comprising: a reference member held in fixed position and orientation with respect to a workpiece during measurement; a stylus for scanning a surface of the workpiece while being displaced upward and downward in accordance with unevenness of the surface of the workpiece; a displacement gauge for measuring a displacement of a specific part of the stylus relative to the reference member; and a scanner for causing the stylus to scan the workpiece along the surface; wherein the fixed position and orientation of the reference member with respect to the workpiece are not changed even during the operation of the scanner; the up-and-down displacement of the specific part of the stylus is measured relative to the reference member; a fine shape of the workpiece is detected in accordance with the measured displacement of the specific part of the stylus.

摘要翻译： 一种测量装置，包括：在测量期间相对于工件保持固定位置和取向的参考构件; 用于根据工件表面的不平坦度向上和向下移动来扫描工件的表面的触针; 用于测量触针的特定部分相对于参考构件的位移的位移计; 以及用于使所述触针沿着所述表面扫描所述工件的扫描仪; 其中，即使在扫描仪的操作期间，参考构件相对于工件的固定位置和取向也不改变; 相对于参考构件测量触针的特定部分的上下移位; 根据测量头的特定部位的位移来检测工件的精细形状。

公开(公告)号：US07570362B2

公开(公告)日：2009-08-04

申请号：US12229128

申请日：2008-08-19

申请人： Shinichi Dosaka ,  Yoshimasa Suzuki

发明人： Shinichi Dosaka ,  Yoshimasa Suzuki

IPC分类号： G01N21/55 ,  G01N21/41

CPC分类号： G01N21/43 ,  G01N21/553

摘要： In optical measurement utilizing total reflection, various types of measurement are selectively performed. The invention provides an optical measurement apparatus using total reflection, including a light source, a measurement optical system, and a light detector. The measurement optical system is an infinity-corrected positive lens formed of an optical member having a planar surface orthogonal to an optical axis of the measurement optical system at a front focal position. One side of the optical axis of the measurement optical system is used as a projection optical system for radiating measurement light onto a specimen, and another side is used as a photometric optical system for acquiring reflected light from the specimen. The light source is disposed at an entrance pupil position on the projection optical system side or at a position conjugate to the entrance pupil position and moves in an entrance pupil plane or in a plane conjugate to the entrance pupil position, along a straight line orthogonal to the optical axis, while a distance from the optical axis is detected. The light detector is disposed at an exit pupil position on the photometric optical system side or at a position conjugate to the exit pupil position. The optical-measurement apparatus comprises a light-source changing unit configured to change the position or shape of the light source.

摘要翻译： 在使用全反射的光学测量中，选择性地执行各种类型的测量。 本发明提供一种使用全反射的光学测量装置，包括光源，测量光学系统和光检测器。 测量光学系统是由在前焦点位置处具有与测量光学系统的光轴正交的平面的光学构件形成的无穷大校正正透镜。 将测量光学系统的光轴的一侧用作用于将测量光照射到样本上的投影光学系统，另一侧用作用于获取来自样本的反射光的光度测量系统。 光源设置在投影光学系统侧的入射光瞳位置或与入射光瞳位置共轭的位置处，并且沿与入射光瞳位置共轭的平面沿着垂直于入射光瞳位置的直线移动 同时检测与光轴的距离。 光检测器设置在测光光学系统侧的出射光瞳位置或与出射光瞳位置共轭的位置。 光学测量装置包括被配置为改变光源的位置或形状的光源改变单元。

公开(公告)号：US07397570B2

公开(公告)日：2008-07-08

申请号：US11433485

申请日：2006-05-15

申请人： Kazuhiko Kawasaki ,  Yoshimasa Suzuki ,  David W. Sesko

发明人： Kazuhiko Kawasaki ,  Yoshimasa Suzuki ,  David W. Sesko

IPC分类号： G01B11/02

CPC分类号： G01B9/02048 ,  G01B9/02004 ,  G01B9/02081 ,  G01B2290/45 ,  G01B2290/70

摘要： A wavelength-variable light source is configured to emit a light with a wavelength (λ), which is variable within a scan width (Δλ). An interferometer has a coherent length (ΔL), which is determinable from (Δλ) and (λ). A controller determines an appropriate magnitude of the scan width (Δλ) while a CCD camera captures a fringe image in an exposure time (Te), which is set longer than a time for wavelength scanning.

摘要翻译： 波长可变光源被配置为发射波长（λ）的光，其在扫描宽度（Deltalambda）内是可变的。 干涉仪具有相干长度（DeltaL），其可以从（Deltalambda）和（λ）确定。 控制器确定扫描宽度（Deltalambda）的合适幅度，而CCD照相机在曝光时间（Te）中拍摄条纹图像，曝光时间（Te）设置为比波长扫描时间长。