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    Expression vector for animal cell line and use thereof
    2.
    发明授权
    Expression vector for animal cell line and use thereof 失效
    动物细胞系的表达载体及其用途

    公开(公告)号：US4935352A

    公开(公告)日：1990-06-19

    申请号：US919798

    申请日：1986-10-16

    申请人： Koichi Igarashi ,  Reiko Sasada ,  Tomoko Fujii ,  Ryuji Marumoto

    发明人： Koichi Igarashi ,  Reiko Sasada ,  Tomoko Fujii ,  Ryuji Marumoto

    IPC分类号： C07K14/485 ,  C07K14/55 ,  C07K16/00 ,  C12N15/85

    CPC分类号： C12N15/85 ,  C07K14/485 ,  C07K14/55 ,  C07K16/00 ,  C07K2319/02

    摘要： An animal cell line transformed with an expression vector for an animal cell, the expression vector which contains a DNA segment comprising:(a) a DNA sequence coding for a signal peptide of an animal cell-derived protein,(b) a second DNA sequence coding for a different protein from the signal protein joined downstream of said signal peptide encoding DNA sequence without causing any reading frame shift, and(c) a promoter DNA sequence capable of functioning in an animal cell, wherein the promoter sequence is positioned upstream of the signal peptide encoding DNA sequence, can produce glycosylated proteins advantageously as secretable proteins.

    摘要翻译： 用动物细胞表达载体转化的动物细胞系，含有DNA片段的表达载体包含：（a）编码动物细胞来源的蛋白质的信号肽的DNA序列，（b）第二DNA序列 编码来自所述信号肽编码DNA序列下游的信号蛋白的不同蛋白质，而不引起任何阅读框移动，和（c）能够在动物细胞中起作用的启动子DNA序列，其中启动子序列位于 信号肽编码DNA序列，可以有利地产生糖基化的蛋白质作为可分泌的蛋白质。

    Production of 2,6-diaminonebularines
    3.
    发明授权
    Production of 2,6-diaminonebularines 失效
    生产2,6-二氨基苯甲酸

    公开(公告)号：US4255565A

    公开(公告)日：1981-03-10

    申请号：US953255

    申请日：1978-10-20

    申请人： Ryuji Marumoto ,  Shunsuke Shima ,  Yoshiyasu Furukawa

    发明人： Ryuji Marumoto ,  Shunsuke Shima ,  Yoshiyasu Furukawa

    IPC分类号： C07H19/16 ,  C07H17/00

    CPC分类号： C07H19/16 ,  Y02P20/55

    摘要： Compounds of the formula ##STR1## wherein R is a phenyl or cyclohexyl group which may be substituted by halogen, lower alkyl or lower alkoxy, or their acid addition salts, are produced in good yield by reacting a compound of the formula ##STR2## wherein R.sup.1, R.sup.2 and R.sup.3, independently of each other, are a hydroxyl group which may be proctected, which is prepared in 2 or 3 steps from 5-amino-1-.beta.-D-ribofuranosylimidazole-4-carboxamide, with a compound of the formulaR--N.dbd.C.dbd.N--R.sup.4wherein R has the same meaning as defined above and R.sup.4 is hydrogen or the same group as R, and, if necessary, subjecting the resulting compound to a treatment for removal of protective groups on its hydroxyl groups.

    摘要翻译： 其中R是可被卤素，低级烷基或低级烷氧基取代的苯基或环己基或其酸加成盐的式“IMAGE”的化合物以良好的收率通过使下式化合物 R 1，R 2和R 3彼此独立地是羟基，其可以被直接制备，其从5-氨基-β-D-呋喃核糖基咪唑-4-甲酰胺以2或3个步骤制备， 式RN = C = N-R4其中R具有与上述相同的含义，R 4是氢或与R相同的基团，如果需要，将所得化合物进行处理以除去其羟基上的保护基。

    DNA encoding recombinant human lymphotoxin
    4.
    发明授权
    DNA encoding recombinant human lymphotoxin 失效
    编码重组人淋巴毒素的DNA

    公开(公告)号：US5175268A

    公开(公告)日：1992-12-29

    申请号：US416657

    申请日：1989-10-03

    申请人： Susumu Iwasa ,  Tomoko Fujii ,  Ryuji Marumoto ,  Koichi Igarashi

    发明人： Susumu Iwasa ,  Tomoko Fujii ,  Ryuji Marumoto ,  Koichi Igarashi

    IPC分类号： C07K14/525 ,  C12N15/19

    CPC分类号： C07K14/525

    摘要： Lymphotoxin (LT) mutein (genetically-altered LT) is disclosed that has the following amino acid sequence, or a portion of an active portion of said protein, where 10 to 21 amino acids of LT being deleted from N-terminus and which has Pro or Phe at the N-terminus: ##STR1## Wherein R.sub.1 is Pro or Phe, R.sub.2 is a peptide chain represented by the following sequence:Ala-Gln-Thr-Ala-Arg-Gln-His-Pro-Lys-Met-His-Leu,or a portion thereof and n is 0 or 1.The LT mutein can be recovered in a higher yield and purified more efficiently under mild conditions which does not harm the LT's biological activity, than the whole LT.

    摘要翻译： 公开了具有以下氨基酸序列或所述蛋白质的活性部分的一部分的淋巴毒素（LT）突变蛋白（遗传改变的LT），其中LT的10至21个氨基酸从N末端缺失并且具有Pro 或N末端的Phe：Ala-His-Leu-Ile-Gly-Asp-Pro-Ser-Lys-Gln-Asn-Ser-Leu-Leu-Trp-Arg-Ala-Asn-Thr-Asp -Arg-Ala-Phe-Leu-Gln-Asp-Gly-Phe-Ser-Leu-Ser-Asn-Asn-Ser-Leu-Leu- Val-Pro-Thr-Ser-Gly-Ile-Tyr-Phe-Val - Tyr-Ser-Gln-Val-Val-Phe-Ser-Gly-Lys-Ala-Tyr-Ser-Pro-Lys-Ala-Thr-Ser-Ser-Pro-Leu-Tyr-Leu-Ala-His-Glu -Val-Gln-Leu-Phe-Ser-Ser-Gln-Tyr-Pro-Phe-His-Val-Pro-Leu-Leu-Ser-Ser-Gln-Lys-Met-Val-Tyr-Pro-Gly-Leu -Gln-Glu-Pro-Trp-Leu-His-Ser-Met-Tyr-His-Gly-Ala-Al-Phe-Gln-Leu-Thr-Gln-Gly-Asp-Gln-Leu-Ser-Thr-His -Thr-Asp-Gly-Ile-Pro-His-Leu-Val-Leu-Ser-Pro-Ser-Thr-Val-Phe-Phe-Gly-Ala-Phe-Ala-Leu-OH其中R1是Pro或Phe R2是由以下序列表示的肽链：Ala-Gln-Thr-Ala-Arg-Gln-His-Pro-Lys-Met-His-Leu或端口 并且n为0或1.可以比在整个LT中更高的产率回收LT突变蛋白，并且在不影响LT的生物活性的温和条件下更有效地纯化。

    2,6-Diaminonebularine derivatives
    6.
    发明授权
    2,6-Diaminonebularine derivatives 失效
    2,6-二氨基鸟苷衍生物

    公开(公告)号：US3936439A

    公开(公告)日：1976-02-03

    申请号：US420380

    申请日：1973-11-30

    申请人： Ryuji Marumoto ,  Yoshio Yoshioka ,  Mikio Honjo ,  Katsuyoshi Kawazoe

    发明人： Ryuji Marumoto ,  Yoshio Yoshioka ,  Mikio Honjo ,  Katsuyoshi Kawazoe

    IPC分类号： C07H19/16

    CPC分类号： C07H19/16 ,  Y10S514/822 ,  Y10S514/929

    摘要： Novel 2,6-diaminonebularine derivatives of the formula ##SPC1##Wherein R is a phenyl or cyclohexyl group which may be substituted with lower alkyl, lower alkoxy or halogen, and pharmaceutically acceptable salts thereof, exhibit excellent pharmacological acitivity such as strong and prolonged coronary dilating and platelet aggregation inhibitory actions.

    摘要翻译： 式WHEREIN R是可被低级烷基，低级烷氧基或卤素取代的苯基或环己基及其药学上可接受的盐的新型2,6-二氨基鸟苷衍生物，具有优异的药理活性，例如强烈和延长的冠状动脉扩张和血小板 聚集抑制作用。

    Production of 2,6-diaminonebularines
    8.
    发明授权
    Production of 2,6-diaminonebularines 失效
    生产2,6-二氨基苯甲酸

    公开(公告)号：US4293690A

    公开(公告)日：1981-10-06

    申请号：US136072

    申请日：1980-03-28

    申请人： Yoichi Sawa ,  Yoshiyuki Kawakami ,  Ryuji Marumoto

    发明人： Yoichi Sawa ,  Yoshiyuki Kawakami ,  Ryuji Marumoto

    IPC分类号： C07H19/167 ,  C07H19/052 ,  C07H19/16 ,  C07H19/18

    CPC分类号： C07H19/16 ,  C07H19/052 ,  Y02P20/55

    摘要： Compounds of the formula ##STR1## wherein R is phenyl which may be substituted; or their acid addition salts, are produced in good yield by reacting a compound of the formula ##STR2## wherein R.sup.1, R.sup.2 and R.sup.3, independently of one another, are hydroxyl which may be protected, which is prepared in 2 or 3 steps from 5-amino-1-.beta.-D-ribofuranosylimidazole-4-carboxamide, with a compound of the formula ##STR3## wherein R has the same meaning as defined above and X is amino which may be substituted or lower alkylthio, and, if necessary subjecting the resulting compound to a treatment for removal of protective groups on its hydroxyls.

    摘要翻译： 其中R是可被取代的苯基的式“IMAGE”的化合物; 或其酸加成盐以良好的产率通过使下式化合物反应而得到：其中R 1，R 2和R 3彼此独立地为可被保护的羟基，其以2或3步从5 - 氨基-1-β-D-呋喃核糖基咪唑-4-甲酰胺，其中R为与上述定义相同的含义，X为可被取代或低级烷硫基的氨基，如果需要， 所得化合物用于除去其羟基上的保护基团的处理。

    2,6-Diaminonebularines
    9.
    发明授权
    2,6-Diaminonebularines 失效
    2,6-二氨基酚

    公开(公告)号：US4258033A

    公开(公告)日：1981-03-24

    申请号：US85057

    申请日：1979-10-12

    申请人： Ryuji Marumoto ,  Masao Tanabe ,  Yoshiyasu Furukawa

    发明人： Ryuji Marumoto ,  Masao Tanabe ,  Yoshiyasu Furukawa

    IPC分类号： C07H19/16 ,  A61K31/70 ,  C07H17/00

    CPC分类号： C07H19/16

    摘要： Novel N.sup.2 -substituted phenyl-2,6-diaminonebularines of the formula ##STR1## wherein either one of R.sup.1 and R.sup.2 is carbamoyl which may be substituted or acyl group and the other is hydrogen atom, halogen atom or lower alkoxyl group, and their acid addition salts have an excellent coronary vasodilator action.

    摘要翻译： 式（I）所示的新型N 2取代的苯基-2,6-二氨基伯布林，其中R 1和R 2中的任一个是可以被取代或酰基的氨基甲酰基，另一个是氢原子，卤原子或低级烷氧基， 加成盐具有优异的冠状血管扩张作用。