公开(公告)号：US07977461B2

公开(公告)日：2011-07-12

申请号：US11912757

申请日：2006-04-28

申请人： Hiroshi Takayama ,  Kamon Shirakawa ,  Shoji Furusako ,  Yoshitaka Hosaka ,  Tomokazu Matsusue ,  Katsuki Naitoh ,  Yumi Hotta ,  Tetsushi Kawahara ,  Motoyasu Honda

发明人： Hiroshi Takayama ,  Kamon Shirakawa ,  Shoji Furusako ,  Yoshitaka Hosaka ,  Tomokazu Matsusue ,  Katsuki Naitoh ,  Yumi Hotta ,  Tetsushi Kawahara ,  Motoyasu Honda

IPC分类号： C12P21/08

CPC分类号： C07K16/28 ,  A61K2039/505 ,  C07K16/2803 ,  C07K16/34 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/75 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/30

摘要： The present invention provides an antibody which has the following features, its active fragment, or a derivative thereof: a) It specifically binds to human platelet membrane glycoprotein VI (GPVI); b) The function to activate a platelet and/or the function to induce a thrombocytopenia in vivo are low; and c) It at least partially depletes GPVI on the platelet membrane by contacting with a platelet.

摘要翻译： 本发明提供具有以下特征的抗体，其活性片段或其衍生物：a）其特异性结合人血小板膜糖蛋白VI（GPVI）; b）激活血小板和/或在体内诱导血小板减少症的功能低; 和c）它通过与血小板接触至少部分地消耗血小板膜上的GPVI。

公开(公告)号：US20130011868A1

公开(公告)日：2013-01-10

申请号：US13617409

申请日：2012-09-14

申请人： Yoshitaka Hosaka ,  Katsuki Naitoh ,  Motoyasu Honda

发明人： Yoshitaka Hosaka ,  Katsuki Naitoh ,  Motoyasu Honda

IPC分类号： G01N33/566

CPC分类号： C07K16/28 ,  C07K2317/34 ,  G01N33/86 ,  G01N2333/70503 ,  G01N2800/32

摘要： The present invention provides a convenient and highly sensitive method of determining sGPVI present in plasma; this is accomplished by establishing a plurality of mouse hybridomas that produce antibody against GPVI and combining the antibodies produced therefrom. Provided thereby are a novel platelet activation marker, a reagent and method for determining this novel platelet activation marker, and novel applications of this marker in, for example, the diagnosis of diseases associated with platelet activation/vascular endothelial injury.

摘要翻译： 本发明提供了一种方便，高灵敏度的测定血浆中存在的sGPVI的方法; 这通过建立产生针对GPVI的抗体并组合由其产生的抗体的多个小鼠杂交瘤来实现。 由此提供了一种新型的血小板活化标记，用于确定该新型血小板活化标记的试剂和方法，以及该标记在例如与血小板活化/血管内皮损伤相关的疾病的诊断中的新应用。

公开(公告)号：US08323905B2

公开(公告)日：2012-12-04

申请号：US12225722

申请日：2007-03-20

申请人： Yoshitaka Hosaka ,  Katsuki Naitoh ,  Motoyasu Honda

发明人： Yoshitaka Hosaka ,  Katsuki Naitoh ,  Motoyasu Honda

IPC分类号： G01N33/53

CPC分类号： C07K16/28 ,  C07K2317/34 ,  G01N33/86 ,  G01N2333/70503 ,  G01N2800/32

摘要： The present invention provides a convenient and highly sensitive method of determining sGPVI present in plasma; this is accomplished by establishing a plurality of mouse hybridomas that produce antibody against GPVI and combining the antibodies produced therefrom. Provided thereby are a novel platelet activation marker, a reagent and method for determining this novel platelet activation marker, and novel applications of this marker in, for example, the diagnosis of diseases associated with platelet activation/vascular endothelial injury.

摘要翻译： 本发明提供了一种方便，高灵敏度的测定血浆中存在的sGPVI的方法; 这通过建立产生针对GPVI的抗体并组合由其产生的抗体的多个小鼠杂交瘤来实现。 由此提供了一种新型的血小板活化标记，用于确定该新型血小板活化标记的试剂和方法，以及该标记在例如与血小板活化/血管内皮损伤相关的疾病的诊断中的新应用。

公开(公告)号：US08389692B2

公开(公告)日：2013-03-05

申请号：US13095098

申请日：2011-04-27

申请人： Hiroshi Takayama ,  Kamon Shirakawa ,  Shoji Furusako ,  Yoshitaka Hosaka ,  Tomokazu Matsusue ,  Katsuki Naitoh ,  Yumi Hotta ,  Tetsushi Kawahara ,  Motoyasu Honda

发明人： Hiroshi Takayama ,  Kamon Shirakawa ,  Shoji Furusako ,  Yoshitaka Hosaka ,  Tomokazu Matsusue ,  Katsuki Naitoh ,  Yumi Hotta ,  Tetsushi Kawahara ,  Motoyasu Honda

IPC分类号： C12P21/08

CPC分类号： C07K16/28 ,  A61K2039/505 ,  C07K16/2803 ,  C07K16/34 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/75 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/30

摘要： The present invention provides an antibody which has the following features, its active fragment, or a derivative thereof: a) It specifically binds to human platelet membrane glycoprotein VI (GPVI); b) The function to activate a platelet and/or the function to induce a thrombocytopenia in vivo are low; and c) It at least partially depletes GPVI on the platelet membrane by contacting with a platelet.

公开(公告)号：US20090311728A1

公开(公告)日：2009-12-17

申请号：US12225722

申请日：2007-03-20

申请人： Yoshitaka Hosaka ,  Katsuki Naitoh ,  Motoyasu Honda

发明人： Yoshitaka Hosaka ,  Katsuki Naitoh ,  Motoyasu Honda

IPC分类号： G01N33/53 ,  C07K16/00

CPC分类号： C07K16/28 ,  C07K2317/34 ,  G01N33/86 ,  G01N2333/70503 ,  G01N2800/32

摘要： The present invention provides a convenient and highly sensitive method of determining sGPVI present in plasma; this is accomplished by establishing a plurality of mouse hybridomas that produce antibody against GPVI and combining the antibodies produced therefrom. Provided thereby are a novel platelet activation marker, a reagent and method for determining this novel platelet activation marker, and novel applications of this marker in, for example, the diagnosis of diseases associated with platelet activation/vascular endothelial injury.

摘要翻译： 本发明提供了一种方便，高灵敏度的测定血浆中存在的sGPVI的方法; 这通过建立产生针对GPVI的抗体并组合由其产生的抗体的多个小鼠杂交瘤来实现。 由此提供了一种新型的血小板活化标记，用于确定该新型血小板活化标记的试剂和方法，以及该标记在例如与血小板活化/血管内皮损伤相关的疾病的诊断中的新应用。

公开(公告)号：US20090092612A1

公开(公告)日：2009-04-09

申请号：US11912757

申请日：2006-04-28

申请人： Hiroshi Takayama ,  Kamon Shirakawa ,  Shoji Furusako ,  Yoshitaka Hosaka ,  Tomokazu Matsusue ,  Katsuki Naitoh ,  Yumi Hotta ,  Tetsushi Kawahara ,  Motoyasu Honda

发明人： Hiroshi Takayama ,  Kamon Shirakawa ,  Shoji Furusako ,  Yoshitaka Hosaka ,  Tomokazu Matsusue ,  Katsuki Naitoh ,  Yumi Hotta ,  Tetsushi Kawahara ,  Motoyasu Honda

IPC分类号： A61K39/395 ,  C07K16/18 ,  C12N5/06 ,  C12N15/11 ,  C12P21/04

CPC分类号： C07K16/28 ,  A61K2039/505 ,  C07K16/2803 ,  C07K16/34 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/75 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/30

摘要： The present invention provides an antibody which has the following features, its active fragment, or a derivative thereof: a) It specifically binds to human platelet membrane glycoprotein VI (GPVI); b) The function to activate a platelet and/or the function to induce a thrombocytopenia in vivo are low; and c) It at least partially depletes GPVI on the platelet membrane by contacting with a platelet.