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1.发明授权Sandwich immunoassay of .beta.-n-acetylglucosaminidase and monoclonal antibody used therein 失效其中使用的β-n-乙酰氨基葡萄糖苷酶和单克隆抗体的三明治免疫测定
公开(公告)号：US5358850A
公开(公告)日：1994-10-25
申请号：US77868
申请日：1993-06-18
申请人： Yoshito Numata , Yoko Kosugi , Kazunori Shibata
发明人： Yoshito Numata , Yoko Kosugi , Kazunori Shibata
IPC分类号： C12N9/52 , G01N33/573 , G01N33/536 , G01N33/543 , G01N33/577
CPC分类号： C12N9/52 , G01N33/573 , G01N2800/52 , Y10S435/971 , Y10S435/975
摘要： The present invention relates to a process of preparing a sandwich immunoassay of NAG, which comprises (1) reacting NAG with an immobilized anti-NAG monoclonal antibody and a labeled anti-NAG monoclonal antibody to form a complex of immobilized antibody-NAG-labeled antibody and (2) detecting the activity of said reacted and unreacted labeled anti-NAG antibody.This sandwich immunoassay is useful for diagnosis of renal disease, hepatitis, leukemia, and other such diseases. It also allows the direct and specific detection of NAG isozymes B and I in urine and blood.
摘要翻译： 本发明涉及一种制备NAG的夹心免疫测定方法，其包括（1）使NAG与固定的抗NAG单克隆抗体和标记的抗NAG单克隆抗体反应以形成固定化抗体-NAG标记抗体的复合物 和（2）检测所述反应和未反应的标记抗NAG抗体的活性。 这种夹心免疫测定法可用于诊断肾病，肝炎，白血病等疾病。 它还允许在尿和血液中直接和特异性检测NAG同功酶B和I。
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公开(公告)号：US20120237948A1
公开(公告)日：2012-09-20
申请号：US13496483
申请日：2010-09-16
申请人： Yoshito Numata , Akira Yamauchi , Junji Onoda , Shoji Yamane , Tomoko Maeda
发明人： Yoshito Numata , Akira Yamauchi , Junji Onoda , Shoji Yamane , Tomoko Maeda
IPC分类号： C07K16/18 , G01N33/573 , G01N33/566
CPC分类号： G01N33/6887 , C07K16/18 , C07K2317/34 , G01N2333/78
摘要： The present invention provides a novel monoclonal antibody that is specific for a C-terminal neoepitope of a collagen fragment and has substantially equal binding affinity whether proline contained in the neoepitope is in a non-hydroxylated form or in a hydroxylated form, and an immunoassay, a measurement method, a kit and the like using the antibody. The antibody allows for quantification of a collagen fragment generated by digestion of a biological sample with a collagenase present in the sample, regardless of the presence or absence of a hydroxylated form of proline in the neoepitope.
摘要翻译： 本发明提供了一种对胶原片段的C末端新表皮特异的新型单克隆抗体，并且具有与新表皮中所含的脯氨酸为非羟基化形式或羟基化形式的免疫测定相同的结合亲和力， 使用抗体的测定方法，试剂盒等。 抗体允许通过用存在于样品中的胶原酶消化生物样品而产生的胶原片段进行定量，而不管新表皮中是否存在羟基化形式的脯氨酸。
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公开(公告)号：US20120093808A1
公开(公告)日：2012-04-19
申请号：US13256219
申请日：2010-03-09
CPC分类号： C07K16/1214 , C07K2317/24 , C07K2317/76 , C07K2317/92
摘要： Provided are a humanized monoclonal antibody against PcrV or a part thereof, and a pharmaceutical composition containing the same as an active ingredient, as an effective means for therapy of infection, particularly infection with Pseudomonas aeruginosa. Concretely, the humanized monoclonal antibody of the present invention has an excellent inhibitory activity on the cytotoxicity with respect to a target cell of Pseudomonas aeruginosa. Also, the humanized monoclonal antibody of the present invention has a high affinity for PcrV.
摘要翻译： 提供了抗PcrV或其一部分的人源化单克隆抗体，以及含有其作为活性成分的药物组合物，作为感染治疗，特别是铜绿假单胞菌感染的有效手段。 具体而言，本发明的人源化单克隆抗体相对于铜绿假单胞菌的靶细胞的细胞毒性具有优异的抑制活性。 此外，本发明的人源化单克隆抗体对PcrV具有高亲和力。
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公开(公告)号：US20090298196A1
公开(公告)日：2009-12-03
申请号：US11814957
申请日：2006-01-26
IPC分类号： G01N33/566 , C12N15/00 , C07K16/18
CPC分类号： C07K16/18 , C07K2317/56 , C12N15/62 , G01N21/6428 , G01N33/542 , G01N33/6803
摘要： The present invention is intended to provide a method of rapidly, simply and accurately measuring a recombinant protein. As means for resolution, the protein quantity is measured by causing the expression of a fusion protein with the target protein and epitope tags containing two types of epitopes, bringing them into contact with detection antibodies which specifically recognize each epitope, and detecting a phenomenon caused by both detection antibodies coming close to each other.
摘要翻译： 本发明旨在提供一种快速，简单且准确地测量重组蛋白质的方法。 作为解决方法，通过使靶蛋白和表位标签含有两种表位的融合蛋白的表达来测量蛋白质量，使其与特异性识别每个表位的检测抗体接触，并检测由 两种检测抗体相互接近。
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公开(公告)号：US08883977B2
公开(公告)日：2014-11-11
申请号：US13643168
申请日：2011-04-28
申请人： Shin-Ichiro Nishimura , Shoichi Naito , Naoki Ohyabu , Tatsuya Takahashi , Yoshito Numata , Keiko Kawamoto
发明人： Shin-Ichiro Nishimura , Shoichi Naito , Naoki Ohyabu , Tatsuya Takahashi , Yoshito Numata , Keiko Kawamoto
IPC分类号： C07K16/18 , A61K39/00 , A61K39/395 , A61K49/16 , G01N33/574 , C07K16/30
CPC分类号： G01N33/57484 , A61K2039/545 , C07K16/3092 , C07K2317/34 , C07K2317/73
摘要： An objective of the present invention is to provide a MUC1 antibody having high specificity to cancer cells. The objective has been achieved by the present inventors who found that a cancer-specific sugar chain can be specifically recognized in MUC1 and a cancer cell expressing MUC1 having such a cancer cell specific sugar chain can be recognized. The present invention provides an antibody, an antigen-binding fragment thereof or a MUC1-binding molecule, having, for example, 40-fold specificity or more for a cancer-associated structure of MUC1 as compared to that of a normal tissue-associated structure of MUC1.
摘要翻译： 本发明的目的是提供对癌细胞具有高特异性的MUC1抗体。 本发明人已经发现，发现可以在MUC1中特异性地识别癌特异性糖链，并且可以认识到具有这样的癌细胞特异性糖链的表达MUC1的癌细胞。 与正常组织相关结构相比，本发明提供了抗体，其抗原结合片段或MUC1结合分子，其与MUC1的癌症相关结构具有例如40倍特异性或更多 的MUC1。
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6.发明申请公开(公告)号：US20060193771A1
公开(公告)日：2006-08-31
申请号：US10516766
申请日：2003-06-11
申请人： Ira Pastan , Satoshi Nagata , Masanori Onda , Yoshito Numata , Kenneth Santora , Richard Beers , Robert Kreitman , Abhishek Sinha
发明人： Ira Pastan , Satoshi Nagata , Masanori Onda , Yoshito Numata , Kenneth Santora , Richard Beers , Robert Kreitman , Abhishek Sinha
IPC分类号： A61K39/395 , A61K51/00 , C07K16/46 , C07K16/30
CPC分类号： C07K16/2878 , A61K47/6829 , A61K47/6849 , A61K51/1027 , A61K2039/505 , C07K2317/622 , C07K2317/624 , C07K2317/734 , C07K2317/92 , C07K2319/00 , C07K2319/30
摘要： CD30 is a receptor expressed on cells of Hodgkin's disease and certain leukemias. The extracellular portion of CD30 is cleaved, releasing a form known as sCD30. The invention relates in part to the discovery that a residual, extracellular “stalk” of CD30 remains after cleavage of sCD30. The stalk provides an advantageous and previously unrecognized target for immunotoxins. The invention provides antibodies that bind to the CD30 stalk or to epitopes destroyed upon the cleavage of CD30 which results in the stalk. The invention further provides new anti-CD30 antibodies that form effective immunotoxins and are particularly suitable for making disulfide stabilized Fv (“dsFv”)-immunoconjugates. The dsFv immunoconjugates can be used as reagents to label CD30-expressing cancer cells or to inhibit the growth of CD30-expressing cancer cells. Moreover, the invention provides anti-CD30 antibodies that activate complement-dependent cytotoxicity.
摘要翻译： CD30是在霍奇金病和某些白血病细胞上表达的受体。 CD30的细胞外部分被切割，释放出称为sCD30的形式。 本发明部分地涉及在sCD30切割后残留的CD30的残留的细胞外“茎”的发现。 茎为免疫毒素提供了有利且以前未被认识的靶标。 本发明提供了结合CD30茎或在导致茎的CD30切割时被破坏的表位的抗体。 本发明进一步提供形成有效免疫毒素的新抗CD30抗体，特别适用于制备二硫键稳定的Fv（“dsFv”） - 免疫偶联物。 dsFv免疫偶联物可用作标记表达CD30的癌细胞的试剂或抑制表达CD30的癌细胞的生长。 此外，本发明提供了激活补体依赖性细胞毒性的抗-CD30抗体。
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公开(公告)号：US08501179B2
公开(公告)日：2013-08-06
申请号：US12863983
申请日：2009-01-08
IPC分类号： A61K39/40
CPC分类号： C07K16/1214 , C07K2317/76 , C07K2317/92
摘要： Provided is an effective means for therapy of infection, particularly infection with Pseudomonas aeruginosa. Provided are a monoclonal antibody against PcrV or a part thereof, and a pharmaceutical composition containing the same as an active ingredient. Concretely, monoclonal antibody of the present invention has excellent inhibiting activity on cytotoxicity with respect to a target cell of Pseudomonas aeruginosa. Also, the monoclonal antibody of the present invention has high affinity with PcrV.
摘要翻译： 提供治疗感染，特别是铜绿假单胞菌感染的有效手段。 提供了针对PcrV或其一部分的单克隆抗体，以及含有其作为活性成分的药物组合物。 具体地，本发明的单克隆抗体相对于铜绿假单胞菌的靶细胞的细胞毒性具有优异的抑制活性。 此外，本发明的单克隆抗体与PcrV具有高亲和力。
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公开(公告)号：US5702910A
公开(公告)日：1997-12-30
申请号：US569461
申请日：1995-12-08
CPC分类号： G01N33/6893 , C07K16/26 , G01N2333/58 , Y10S435/96 , Y10S436/808 , Y10S436/811 , Y10S530/808 , Y10S530/809 , Y10S530/829
摘要： The present invention relates to a sandwich immunoassay for rapidly and readily measuring N-peptide using two kinds of monoclonal antibodies recognizing different portions of the N-peptide. The method for measuring N-peptide or a precursor thereof includes the steps of: incubating a mixture containing a sample and a first monoclonal antibody recognizing a portion of N-peptide; adding a labelled second monoclonal antibody recognizing a portion of N-peptide to the mixture, followed by further incubation; and detecting the resulting antigen-antibody complex in the mixture. Alternatively, the method includes the steps of: incubating a mixture containing a sample, a first monoclonal antibody recognizing a portion of N-peptide, and a labelled second monoclonal antibody recognizing another portion of N-peptide; and detecting the resulting antigen-antibody complex.
摘要翻译： 本发明涉及使用识别N-肽的不同部分的两种单克隆抗体快速且容易地测量N-肽的夹心免疫测定。 用于测量N-肽或其前体的方法包括以下步骤：将含有样品和识别N肽部分的第一单克隆抗体的混合物孵育; 将识别一部分N-肽的标记的第二单克隆抗体加入到混合物中，随后进一步培养; 并检测混合物中得到的抗原 - 抗体复合物。 或者，该方法包括以下步骤：将含有样品的混合物，识别N肽部分的第一单克隆抗体和识别N-肽的另一部分的标记的第二单克隆抗体孵育; 并检测所得到的抗原 - 抗体复合物。
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公开(公告)号：US08536313B2
公开(公告)日：2013-09-17
申请号：US12668201
申请日：2008-07-08
申请人： Shoichi Naito , Junji Onoda , Akira Yamauchi , Yoshito Numata , Junji Kishono
发明人： Shoichi Naito , Junji Onoda , Akira Yamauchi , Yoshito Numata , Junji Kishono
IPC分类号： C07K16/40 , C07K16/00 , A61K39/395
CPC分类号： G01N33/573 , C07K16/40 , C07K2317/76 , G01N2333/8146
摘要： A neutralizing monoclonal antibody specifically reacting with MMP13, a method of neutralizing enzyme activity of MMP13 and an immunological measuring method each using the antibody, as well as a diagnostic agent and a pharmaceutical composition containing the antibody, are provided. Various antibodies to MMP13 have been hitherto obtained, but an antibody having neutralizing activity against MMP13 has not been obtained. The present inventors intensively studied, as a result, found out a neutralizing antibody having specificity for MMP13, resulting in completion of the present invention.
摘要翻译： 提供了与MMP13特异性反应的中和单克隆抗体，中和MMP13的酶活性的方法和各自使用该抗体的免疫学测定方法，以及含有该抗体的诊断剂和药物组合物。 迄今为止已经获得了MMP13的各种抗体，但是尚未获得具有针对MMP13的中和活性的抗体。 本发明人深入研究了结果，发现了对MMP13具有特异性的中和抗体，从而完成了本发明。
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公开(公告)号：US20130045543A1
公开(公告)日：2013-02-21
申请号：US13643168
申请日：2011-04-28
IPC分类号： C07K16/30 , G01N33/574 , C07H21/04
CPC分类号： G01N33/57484 , A61K2039/545 , C07K16/3092 , C07K2317/34 , C07K2317/73
摘要： An objective of the present invention is to provide a MUC1 antibody having high specificity to cancer cells. The objective has been achieved by the present inventors who found that a cancer-specific sugar chain can be specifically recognized in MUC1 and a cancer cell expressing MUC1 having such a cancer cell specific sugar chain can be recognized. The present invention provides an antibody, an antigen-binding fragment thereof or a MUC1-binding molecule, having, for example, 40-fold specificity or more for a cancer-associated structure of MUC1 as compared to that of a normal tissue-associated structure of MUC1.
摘要翻译： 本发明的目的是提供对癌细胞具有高特异性的MUC1抗体。 本发明人已经发现，发现可以在MUC1中特异性地识别癌特异性糖链，并且可以认识到具有这样的癌细胞特异性糖链的表达MUC1的癌细胞。 与正常组织相关结构相比，本发明提供了抗体，其抗原结合片段或MUC1结合分子，其与MUC1的癌症相关结构具有例如40倍特异性或更多 的MUC1。
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