Method for recovery of refined .alpha.-galactosidase
    3.
    发明授权
    Method for recovery of refined .alpha.-galactosidase 失效
    回收精制{60-半乳糖苷酶的方法

    公开(公告)号:US3972777A

    公开(公告)日:1976-08-03

    申请号:US572944

    申请日:1975-04-30

    IPC分类号: C12N9/40 C07G7/028

    CPC分类号: C12N9/2465 Y10S435/815

    摘要: A method for the recovery of refined .alpha.-galactosidase is disclosed. This method comprises the steps of bringing an .alpha.-galactosidase-containing liquid into contact with a weakly acidic cation-exchange resin in the presence of a buffer solution for thereby allowing .alpha.-galactosidase to be selectively adsorbed by said resin, desorbing the adsorbed .alpha.-galactosidase from said resin by use of a buffer solution of a type such that at least one of the two factors, concentration and pH, has a higher value than that of the buffer solution used in said adsorption and thereafter recovering the desorbed .alpha.-galactosidase.

    摘要翻译: 公开了一种回收精制α-半乳糖苷酶的方法。 该方法包括在缓冲溶液存在下使含有α-半乳糖苷酶的液体与弱酸性阳离子交换树脂接触的步骤,从而使α-半乳糖苷酶被所述树脂选择性吸附,解吸所吸附的α- 通过使用这样的缓冲溶液使得所述树脂的半乳糖苷酶的浓度和pH值中的至少一个比所述吸附中使用的缓冲溶液的值高,然后回收解吸的α-半乳糖苷酶。

    Yeast strain with high power to produce alcohol by fermentation
    4.
    发明授权
    Yeast strain with high power to produce alcohol by fermentation 失效
    酵母菌通过发酵生产酒精

    公开(公告)号:US4910144A

    公开(公告)日:1990-03-20

    申请号:US139786

    申请日:1987-12-30

    IPC分类号: C12P7/06

    摘要: A yeast strain with high power to produce alcohol by fermentation is produced by a method comprising directly conjugating the haploid of the strain No. 909-1 with each tetrad of the ascospores of the strain No. 180 by spore-to-cell mating, acclimating the resultant zygote in the medium of waste after alcohol fermentation, and acclimating the surviving cells of the zygote in the medium of the beet molasses containing 2-deoxyglucose. The yeast strain produced is designated as Strain M-9 (FERM BP 1481) of genus Saccharomyces cerevisiae. Alcohol is produced by culturing the Strain M-9 in a medium of beet molasses.

    摘要翻译: 通过发酵方法产生具有高功率产生醇的酵母菌株,其方法包括通过孢子对细胞交配直接将菌株No.909-1的单倍体与180号菌株的子囊孢子的四分之一缀合,适应 在酒精发酵后的废物培养基中产生的受精卵合并,并适应含有2-脱氧葡萄糖的甜菜糖蜜的培养基中受精卵的存活细胞。 产生的酵母菌株被命名为酿酒酵母属的菌株M-9(FERM BP1481)。 通过在甜菜糖蜜介质中培养菌株M-9产生醇。