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公开(公告)号：US08039256B2
公开(公告)日：2011-10-18
申请号：US12892223
申请日：2010-09-28
CPC分类号： C12N5/0663 , C12N2500/50
摘要： To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring.
摘要翻译： 为了有效地选择和增殖间充质干细胞而不需要专门的分离装置和复杂的分离操作，通过种植骨髓溶液，脐带血，外周血，滑膜中的至少一种来培养间充质干细胞 和填充在容器中的液体培养基中的羊膜包含水作为主要成分，在37℃下的比重为1.06〜1.10，培养温度为37±2℃。 在容器的顶棚侧表面上，优选使用选自由聚乙烯吡咯烷酮涂覆的二氧化硅细粉末，蔗糖和表氯醇的水溶性共聚物和包含三碘芳环的水溶性化合物中的至少一种来调节比重 。
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公开(公告)号：US20090186412A1
公开(公告)日：2009-07-23
申请号：US12356769
申请日：2009-01-21
IPC分类号： C12N5/02
CPC分类号： C12N5/0655 , C12N5/0062 , C12N5/0653 , C12N5/0654 , C12N2533/30 , C12N2533/40
摘要： For easily seeding cells in its scaffold, a porous cell scaffold is produced by steps of filling a guiding solution with kinematic viscosity being 50 to 450% of that of a culture medium, in a whole continuous small hole structure having hole diameters of 5 to 3200 μm and an average hole diameter of 50 to 1500 μm, of a sheet-shaped or block-shaped scaffold having a thickness of 2 mm or more, supplying thereafter a culture medium with cells being suspended to an upper side of the scaffold, sucking the guiding solution from a lower side of the scaffold by low suction force, and entering thereby the culture medium with cells being suspended into the whole small hole structure, where a water absorber such as a filter paper is preferably used for sucking the guiding solution by low suction force.
摘要翻译： 为了容易地在其支架中接种细胞，通过以孔径为5至3200的整个连续的小孔结构填充具有培养基的运动粘度为50至450％的引导溶液的步骤来生产多孔细胞支架 平均孔径为50〜1500μm的片状或块状支架，其厚度为2mm以上，向培养基供给细胞悬浮于支架的上侧，吸附 通过低抽吸力从支架的下侧引导溶液，并由此进入细胞悬浮在整个小孔结构中的培养基，其中优选使用诸如滤纸的吸水剂用于以低的速度吸引引导溶液 吸力。
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公开(公告)号：US20090246873A1
公开(公告)日：2009-10-01
申请号：US12415285
申请日：2009-03-31
IPC分类号： C12N5/06
摘要： To provide a scaffold for tissue engineering which consists of a bioabsorbable polymer material to be absorbed in a biotissue, and holds strength of the whole scaffold while having a porosity proper for culturing cells inside thereof as well, a method for producing the scaffold for tissue engineering includes steps of dissolving a bioabsorbable polymer material with an organic solvent, drying the solution so as to produce a porous bioabsorbable polymer material having a porosity of 50 to 99%, covering the porous bioabsorbable polymer material with a bioabsorbable polymer material having a thickness of 0.01 to 5 mm, pores of 10 to 3000 μm diameter, a fracture strength of 0.05 to 0.15 MPa, and a volume of 15 to 90% with respect to the whole scaffold.
摘要翻译： 提供一种用于组织工程的支架，其由吸收在生物组织中的生物可吸收聚合物材料组成，并且保持整个支架的强度同时具有适合于在其内部培养细胞的孔隙度，一种用于组织工程支架的生产方法 包括将生物可吸收聚合物材料与有机溶剂一起溶解的步骤，干燥该溶液以产生孔隙率为50至99％的多孔生物可吸收聚合物材料，用具有厚度为0.01的生物可吸收聚合物材料覆盖多孔生物可吸收聚合物材料 至5mm，孔径为10-3000μm直径，断裂强度为0.05-0.15MPa，体积相对于整个支架为15-90％。
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